Antileishmanial activity of a mixture of Tridax procumbens and Allium sativum in mice

Antileishmanial activity of a mixture of Tridax procumbens and Allium sativum in mice

We tested a mixture of Tridax procumbens, known for its direct action against Leishmania mexicana, and Allium sativum, known for its immunomodulatory effect, as an alternative to treat cutaneous leishmaniasis. Acute oral toxicity was tested with the Up-and-Down Procedure (UDP) using a group of healthy mice administered with either T. procumbens or A. sativum extracts and compared with a control group. Liver injury and other parameters of toxicity were determined in mice at day 14. The in vivo assay was performed with mice infected with L. mexicana promastigotes and treated with either a mixture of T. procumbens and A. sativum or each extract separately. The thickness of the mice's footpads was measured weekly. After the 12-week period of infection, blood samples were obtained by cardiac puncture to determine the total IgG, IgG1 and IgG2a immunoglobulins by a noncommercial indirect ELISA. We showed that the mixture of T. procumbens and A. sativum extracts was better at controlling L. mexicana infection while not being toxic when tested in the acute oral toxicity assay in mice. An increase in the ratio of IgG2a/IgG1 indicated a tendency to raise a Th1-type immune response in mice treated with the mixture. The mixture of T. procumbens and A. sativum extracts is a promising natural treatment for cutaneous leishmaniasis and its healing effects make it a good candidate for a possible new phytomedicine.

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Main Authors: MIRIAM RUBI DE LOS MILAGROS GAMBOA LEON, BLANCA MARINA VERA KU, SERGIO RUBEN PERAZA SANCHEZ, CARLOS ALBERTO KU CHULIM, AURELIO HORTA BAAS, MIGUEL ENRIQUE ROSADO VALLADO
Format: info:eu-repo/semantics/article biblioteca
Language:eng
Subjects:info:eu-repo/classification/Autores/LEISHMANICIDAL ACTIVITY, info:eu-repo/classification/Autores/TRIDAX PROCUMBENS, info:eu-repo/classification/Autores/ALLIUM SATIVUM, info:eu-repo/classification/Autores/ACUTE ORAL TOXICITY, info:eu-repo/classification/Autores/PHYTOMEDICINE, info:eu-repo/classification/cti/2,
Online Access:http://cicy.repositorioinstitucional.mx/jspui/handle/1003/544
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spelling dig-cicy-1003-5442017-08-11T15:54:57Z Antileishmanial activity of a mixture of Tridax procumbens and Allium sativum in mice Activité antileishmaniale d'un mélange de Tridax procumbens et Allium sativum chez la souris MIRIAM RUBI DE LOS MILAGROS GAMBOA LEON BLANCA MARINA VERA KU SERGIO RUBEN PERAZA SANCHEZ CARLOS ALBERTO KU CHULIM AURELIO HORTA BAAS MIGUEL ENRIQUE ROSADO VALLADO 2014 info:eu-repo/semantics/article We tested a mixture of Tridax procumbens, known for its direct action against Leishmania mexicana, and Allium sativum, known for its immunomodulatory effect, as an alternative to treat cutaneous leishmaniasis. Acute oral toxicity was tested with the Up-and-Down Procedure (UDP) using a group of healthy mice administered with either T. procumbens or A. sativum extracts and compared with a control group. Liver injury and other parameters of toxicity were determined in mice at day 14. The in vivo assay was performed with mice infected with L. mexicana promastigotes and treated with either a mixture of T. procumbens and A. sativum or each extract separately. The thickness of the mice's footpads was measured weekly. After the 12-week period of infection, blood samples were obtained by cardiac puncture to determine the total IgG, IgG1 and IgG2a immunoglobulins by a noncommercial indirect ELISA. We showed that the mixture of T. procumbens and A. sativum extracts was better at controlling L. mexicana infection while not being toxic when tested in the acute oral toxicity assay in mice. An increase in the ratio of IgG2a/IgG1 indicated a tendency to raise a Th1-type immune response in mice treated with the mixture. The mixture of T. procumbens and A. sativum extracts is a promising natural treatment for cutaneous leishmaniasis and its healing effects make it a good candidate for a possible new phytomedicine. info:eu-repo/classification/Autores/LEISHMANICIDAL ACTIVITY info:eu-repo/classification/Autores/TRIDAX PROCUMBENS info:eu-repo/classification/Autores/ALLIUM SATIVUM info:eu-repo/classification/Autores/ACUTE ORAL TOXICITY info:eu-repo/classification/Autores/PHYTOMEDICINE info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 Parasite, 21 doi:10.1051/parasite/2014016 http://cicy.repositorioinstitucional.mx/jspui/handle/1003/544 info:eu-repo/semantics/datasetDOI/DOI: 10.1051/parasite/2014016 info:eu-repo/semantics/openAccess eng citation:Gamboa-Leon, R., Vera-Ku, M., Peraza-Sanchez, S. R., Ku-Chulim, C., Horta-Baas, A., & Rosado-Vallado, M. (2014). Antileishmanial activity of a mixture of tridax procumbens and allium sativum in mice. [Activité antileishmaniale d'un mélange de Tridax procumbens et Allium sativum chez la souris] Parasite, 21 doi:10.1051/parasite/2014016 http://creativecommons.org/licenses/by-nc-nd/4.0 info:eu-repo/semantics/publishedVersion application/pdf
institution CICY
collection DSpace
country México
countrycode MX
component Bibliográfico
access En linea
databasecode dig-cicy
tag biblioteca
region America del Norte
libraryname Biblioteca del CICY
language eng
topic info:eu-repo/classification/Autores/LEISHMANICIDAL ACTIVITY
info:eu-repo/classification/Autores/TRIDAX PROCUMBENS
info:eu-repo/classification/Autores/ALLIUM SATIVUM
info:eu-repo/classification/Autores/ACUTE ORAL TOXICITY
info:eu-repo/classification/Autores/PHYTOMEDICINE
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/2
info:eu-repo/classification/Autores/LEISHMANICIDAL ACTIVITY
info:eu-repo/classification/Autores/TRIDAX PROCUMBENS
info:eu-repo/classification/Autores/ALLIUM SATIVUM
info:eu-repo/classification/Autores/ACUTE ORAL TOXICITY
info:eu-repo/classification/Autores/PHYTOMEDICINE
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/2
spellingShingle info:eu-repo/classification/Autores/LEISHMANICIDAL ACTIVITY
info:eu-repo/classification/Autores/TRIDAX PROCUMBENS
info:eu-repo/classification/Autores/ALLIUM SATIVUM
info:eu-repo/classification/Autores/ACUTE ORAL TOXICITY
info:eu-repo/classification/Autores/PHYTOMEDICINE
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/2
info:eu-repo/classification/Autores/LEISHMANICIDAL ACTIVITY
info:eu-repo/classification/Autores/TRIDAX PROCUMBENS
info:eu-repo/classification/Autores/ALLIUM SATIVUM
info:eu-repo/classification/Autores/ACUTE ORAL TOXICITY
info:eu-repo/classification/Autores/PHYTOMEDICINE
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/2
MIRIAM RUBI DE LOS MILAGROS GAMBOA LEON
BLANCA MARINA VERA KU
SERGIO RUBEN PERAZA SANCHEZ
CARLOS ALBERTO KU CHULIM
AURELIO HORTA BAAS
MIGUEL ENRIQUE ROSADO VALLADO
Antileishmanial activity of a mixture of Tridax procumbens and Allium sativum in mice
description We tested a mixture of Tridax procumbens, known for its direct action against Leishmania mexicana, and Allium sativum, known for its immunomodulatory effect, as an alternative to treat cutaneous leishmaniasis. Acute oral toxicity was tested with the Up-and-Down Procedure (UDP) using a group of healthy mice administered with either T. procumbens or A. sativum extracts and compared with a control group. Liver injury and other parameters of toxicity were determined in mice at day 14. The in vivo assay was performed with mice infected with L. mexicana promastigotes and treated with either a mixture of T. procumbens and A. sativum or each extract separately. The thickness of the mice's footpads was measured weekly. After the 12-week period of infection, blood samples were obtained by cardiac puncture to determine the total IgG, IgG1 and IgG2a immunoglobulins by a noncommercial indirect ELISA. We showed that the mixture of T. procumbens and A. sativum extracts was better at controlling L. mexicana infection while not being toxic when tested in the acute oral toxicity assay in mice. An increase in the ratio of IgG2a/IgG1 indicated a tendency to raise a Th1-type immune response in mice treated with the mixture. The mixture of T. procumbens and A. sativum extracts is a promising natural treatment for cutaneous leishmaniasis and its healing effects make it a good candidate for a possible new phytomedicine.
format info:eu-repo/semantics/article
topic_facet info:eu-repo/classification/Autores/LEISHMANICIDAL ACTIVITY
info:eu-repo/classification/Autores/TRIDAX PROCUMBENS
info:eu-repo/classification/Autores/ALLIUM SATIVUM
info:eu-repo/classification/Autores/ACUTE ORAL TOXICITY
info:eu-repo/classification/Autores/PHYTOMEDICINE
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/2
author MIRIAM RUBI DE LOS MILAGROS GAMBOA LEON
BLANCA MARINA VERA KU
SERGIO RUBEN PERAZA SANCHEZ
CARLOS ALBERTO KU CHULIM
AURELIO HORTA BAAS
MIGUEL ENRIQUE ROSADO VALLADO
author_facet MIRIAM RUBI DE LOS MILAGROS GAMBOA LEON
BLANCA MARINA VERA KU
SERGIO RUBEN PERAZA SANCHEZ
CARLOS ALBERTO KU CHULIM
AURELIO HORTA BAAS
MIGUEL ENRIQUE ROSADO VALLADO
author_sort MIRIAM RUBI DE LOS MILAGROS GAMBOA LEON
title Antileishmanial activity of a mixture of Tridax procumbens and Allium sativum in mice
title_short Antileishmanial activity of a mixture of Tridax procumbens and Allium sativum in mice
title_full Antileishmanial activity of a mixture of Tridax procumbens and Allium sativum in mice
title_fullStr Antileishmanial activity of a mixture of Tridax procumbens and Allium sativum in mice
title_full_unstemmed Antileishmanial activity of a mixture of Tridax procumbens and Allium sativum in mice
title_sort antileishmanial activity of a mixture of tridax procumbens and allium sativum in mice
url http://cicy.repositorioinstitucional.mx/jspui/handle/1003/544
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