Isolation and identification of cytotoxic compounds from Aeschynomene fascicularis, a Mayan medicinal plant
The plant Aeschynomene fascicularis (Fabaceae) has been used in Mayan traditional medicine in the Yucatan peninsula. However, the compounds present in the plant responsible for its curative properties have not yet been investigated. Aeschynomene fascicularis root bark was extracted with 100% methanol to obtain a crude extract. The methanol extract was partitioned successively with solvents with increasing polarity to obtain the corresponding hexane (Hx), dichloromethane (DCM) and ethyl acetate fractions (EtOAc), as well as a residual water-alcoholic fraction. These fractions were tested for their cytotoxic activities using an MTT assay against Hep-2 cancer cell lines. The Hx fraction led to the isolation of spinochalcone C (1), spinochalcone A (2), isocordoin (3) and secundiflorol G (4). Their structures were identified based on spectroscopic evidence and chemical properties. All compounds were subjected to cytotoxicity and antiproliferative assays against a panel of seven cell lines, including one normal-type cell line. Spinochalcone A (2) exhibited cytotoxic activity against DU-145 cell line and antiproliferative activity against the KB cell line. Secundiflorol G (4) showed strong cytotoxic activity towards KB and Hep-2 cell lines. In addition, isocordoin (3) showed moderate activity on KB, Hep-2 and DU-145 cell lines. The active Compounds 2, 3 and 4 are potential therapeutic entities against cancer.
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dig-cicy-1003-5162017-08-07T17:07:21Z Isolation and identification of cytotoxic compounds from Aeschynomene fascicularis, a Mayan medicinal plant ROSA ESTHER MOO PUC EDGAR EMMANUEL CAAMAL FUENTES SERGIO RUBEN PERAZA SANCHEZ LUIS WILIUNFO TORRES TAPIA 2015-08-01 info:eu-repo/semantics/article The plant Aeschynomene fascicularis (Fabaceae) has been used in Mayan traditional medicine in the Yucatan peninsula. However, the compounds present in the plant responsible for its curative properties have not yet been investigated. Aeschynomene fascicularis root bark was extracted with 100% methanol to obtain a crude extract. The methanol extract was partitioned successively with solvents with increasing polarity to obtain the corresponding hexane (Hx), dichloromethane (DCM) and ethyl acetate fractions (EtOAc), as well as a residual water-alcoholic fraction. These fractions were tested for their cytotoxic activities using an MTT assay against Hep-2 cancer cell lines. The Hx fraction led to the isolation of spinochalcone C (1), spinochalcone A (2), isocordoin (3) and secundiflorol G (4). Their structures were identified based on spectroscopic evidence and chemical properties. All compounds were subjected to cytotoxicity and antiproliferative assays against a panel of seven cell lines, including one normal-type cell line. Spinochalcone A (2) exhibited cytotoxic activity against DU-145 cell line and antiproliferative activity against the KB cell line. Secundiflorol G (4) showed strong cytotoxic activity towards KB and Hep-2 cell lines. In addition, isocordoin (3) showed moderate activity on KB, Hep-2 and DU-145 cell lines. The active Compounds 2, 3 and 4 are potential therapeutic entities against cancer. info:eu-repo/classification/Autores/FABACEAE info:eu-repo/classification/Autores/FLAVONOIDS info:eu-repo/classification/Autores/MAYAN MEDICINAL PLANT info:eu-repo/classification/Autores/ANTIPROLIFERATIVE ACTIVITY info:eu-repo/classification/Autores/CYTOTOXIC ACTIVITY info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 Molecules. 20(8), 13563-13574, 2015 http://cicy.repositorioinstitucional.mx/jspui/handle/1003/516 info:eu-repo/semantics/datasetDOI/DOI: 10.3390/molecules200813563 info:eu-repo/semantics/openAccess eng citation:Caamal-Fuentes, E. E., Peraza-Sánchez, S. R., Torres-Tapia, L. W., & Moo-Puc, R. E. (2015). Isolation and identification of cytotoxic compounds from Aeschynomene fascicularis, a Mayan medicinal plant. Molecules, 20(8), 13563-13574. http://creativecommons.org/licenses/by-nc-nd/4.0 info:eu-repo/semantics/publishedVersion application/pdf |
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info:eu-repo/classification/Autores/FABACEAE info:eu-repo/classification/Autores/FLAVONOIDS info:eu-repo/classification/Autores/MAYAN MEDICINAL PLANT info:eu-repo/classification/Autores/ANTIPROLIFERATIVE ACTIVITY info:eu-repo/classification/Autores/CYTOTOXIC ACTIVITY info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 info:eu-repo/classification/Autores/FABACEAE info:eu-repo/classification/Autores/FLAVONOIDS info:eu-repo/classification/Autores/MAYAN MEDICINAL PLANT info:eu-repo/classification/Autores/ANTIPROLIFERATIVE ACTIVITY info:eu-repo/classification/Autores/CYTOTOXIC ACTIVITY info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 |
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info:eu-repo/classification/Autores/FABACEAE info:eu-repo/classification/Autores/FLAVONOIDS info:eu-repo/classification/Autores/MAYAN MEDICINAL PLANT info:eu-repo/classification/Autores/ANTIPROLIFERATIVE ACTIVITY info:eu-repo/classification/Autores/CYTOTOXIC ACTIVITY info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 info:eu-repo/classification/Autores/FABACEAE info:eu-repo/classification/Autores/FLAVONOIDS info:eu-repo/classification/Autores/MAYAN MEDICINAL PLANT info:eu-repo/classification/Autores/ANTIPROLIFERATIVE ACTIVITY info:eu-repo/classification/Autores/CYTOTOXIC ACTIVITY info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 ROSA ESTHER MOO PUC Isolation and identification of cytotoxic compounds from Aeschynomene fascicularis, a Mayan medicinal plant |
| description |
The plant Aeschynomene fascicularis (Fabaceae) has been used in Mayan traditional medicine in the Yucatan peninsula. However, the compounds present in the plant responsible for its curative properties have not yet been investigated. Aeschynomene fascicularis root bark was extracted with 100% methanol to obtain a crude extract. The methanol extract was partitioned successively with solvents with increasing polarity to obtain the corresponding hexane (Hx), dichloromethane (DCM) and ethyl acetate fractions (EtOAc), as well as a residual water-alcoholic fraction. These fractions were tested for their cytotoxic activities using an MTT assay against Hep-2 cancer cell lines. The Hx fraction led to the isolation of spinochalcone C (1), spinochalcone A (2), isocordoin (3) and secundiflorol G (4). Their structures were identified based on spectroscopic evidence and chemical properties. All compounds were subjected to cytotoxicity and antiproliferative assays against a panel of seven cell lines, including one normal-type cell line. Spinochalcone A (2) exhibited cytotoxic activity against DU-145 cell line and antiproliferative activity against the KB cell line. Secundiflorol G (4) showed strong cytotoxic activity towards KB and Hep-2 cell lines. In addition, isocordoin (3) showed moderate activity on KB, Hep-2 and DU-145 cell lines. The active Compounds 2, 3 and 4 are potential therapeutic entities against cancer. |
| author2 |
EDGAR EMMANUEL CAAMAL FUENTES |
| author_facet |
EDGAR EMMANUEL CAAMAL FUENTES ROSA ESTHER MOO PUC |
| format |
info:eu-repo/semantics/article |
| topic_facet |
info:eu-repo/classification/Autores/FABACEAE info:eu-repo/classification/Autores/FLAVONOIDS info:eu-repo/classification/Autores/MAYAN MEDICINAL PLANT info:eu-repo/classification/Autores/ANTIPROLIFERATIVE ACTIVITY info:eu-repo/classification/Autores/CYTOTOXIC ACTIVITY info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 |
| author |
ROSA ESTHER MOO PUC |
| author_sort |
ROSA ESTHER MOO PUC |
| title |
Isolation and identification of cytotoxic compounds from Aeschynomene fascicularis, a Mayan medicinal plant |
| title_short |
Isolation and identification of cytotoxic compounds from Aeschynomene fascicularis, a Mayan medicinal plant |
| title_full |
Isolation and identification of cytotoxic compounds from Aeschynomene fascicularis, a Mayan medicinal plant |
| title_fullStr |
Isolation and identification of cytotoxic compounds from Aeschynomene fascicularis, a Mayan medicinal plant |
| title_full_unstemmed |
Isolation and identification of cytotoxic compounds from Aeschynomene fascicularis, a Mayan medicinal plant |
| title_sort |
isolation and identification of cytotoxic compounds from aeschynomene fascicularis, a mayan medicinal plant |
| url |
http://cicy.repositorioinstitucional.mx/jspui/handle/1003/516 |
| work_keys_str_mv |
AT rosaesthermoopuc isolationandidentificationofcytotoxiccompoundsfromaeschynomenefascicularisamayanmedicinalplant |
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1756089381986238464 |