Dynamics of the concentration of IAA and some of its conjugates during the induction of somatic embryogenesis in Coffea canephora
Most of the somatic embryogenesis (SE) process requires the presence, either before or during the embryogenic process, of at least one exogenous auxin. This exogenous auxin induces the presence of endogenous auxins, which appears to be essential for SE induction. We found that during the preincubation period of SE in Coffea canephora, there is an important increase in both free and conjugated indole-3-acetic acid (IAA), as well as indole-3-butyric acid. This increase is accompanied by an increase in the expression of YUCCA (CcYUC), TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS 1 (CcTAA1), and GRETCHEN HAGEN 3 (GH3) genes. On the other hand, most of the IAA compounds decreased during the induction of SE. The results presented in this research suggest that a balance between free IAA and its amide conjugates is necessary to allow the expression of SE-related genes.
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dig-cicy-1003-402016-10-21T22:23:06Z Dynamics of the concentration of IAA and some of its conjugates during the induction of somatic embryogenesis in Coffea canephora VICTOR MANUEL LOYOLA VARGAS 2013-12-03 info:eu-repo/semantics/article Most of the somatic embryogenesis (SE) process requires the presence, either before or during the embryogenic process, of at least one exogenous auxin. This exogenous auxin induces the presence of endogenous auxins, which appears to be essential for SE induction. We found that during the preincubation period of SE in Coffea canephora, there is an important increase in both free and conjugated indole-3-acetic acid (IAA), as well as indole-3-butyric acid. This increase is accompanied by an increase in the expression of YUCCA (CcYUC), TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS 1 (CcTAA1), and GRETCHEN HAGEN 3 (GH3) genes. On the other hand, most of the IAA compounds decreased during the induction of SE. The results presented in this research suggest that a balance between free IAA and its amide conjugates is necessary to allow the expression of SE-related genes. info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 http://cicy.repositorioinstitucional.mx/jspui/handle/1003/40 info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0 application/pdf |
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info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 VICTOR MANUEL LOYOLA VARGAS Dynamics of the concentration of IAA and some of its conjugates during the induction of somatic embryogenesis in Coffea canephora |
| description |
Most of the somatic embryogenesis (SE) process requires the presence, either before or during the embryogenic
process, of at least one exogenous auxin. This exogenous auxin induces the presence of endogenous auxins, which
appears to be essential for SE induction. We found that during the preincubation period of SE in Coffea canephora,
there is an important increase in both free and conjugated indole-3-acetic acid (IAA), as well as indole-3-butyric acid.
This increase is accompanied by an increase in the expression of YUCCA (CcYUC), TRYPTOPHAN AMINOTRANSFERASE
OF ARABIDOPSIS 1 (CcTAA1), and GRETCHEN HAGEN 3 (GH3) genes. On the other hand, most of the IAA compounds
decreased during the induction of SE. The results presented in this research suggest that a balance between free IAA
and its amide conjugates is necessary to allow the expression of SE-related genes. |
| format |
info:eu-repo/semantics/article |
| topic_facet |
info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 |
| author |
VICTOR MANUEL LOYOLA VARGAS |
| author_facet |
VICTOR MANUEL LOYOLA VARGAS |
| author_sort |
VICTOR MANUEL LOYOLA VARGAS |
| title |
Dynamics of the concentration of IAA and some of its conjugates during the induction of somatic embryogenesis in Coffea canephora |
| title_short |
Dynamics of the concentration of IAA and some of its conjugates during the induction of somatic embryogenesis in Coffea canephora |
| title_full |
Dynamics of the concentration of IAA and some of its conjugates during the induction of somatic embryogenesis in Coffea canephora |
| title_fullStr |
Dynamics of the concentration of IAA and some of its conjugates during the induction of somatic embryogenesis in Coffea canephora |
| title_full_unstemmed |
Dynamics of the concentration of IAA and some of its conjugates during the induction of somatic embryogenesis in Coffea canephora |
| title_sort |
dynamics of the concentration of iaa and some of its conjugates during the induction of somatic embryogenesis in coffea canephora |
| url |
http://cicy.repositorioinstitucional.mx/jspui/handle/1003/40 |
| work_keys_str_mv |
AT victormanuelloyolavargas dynamicsoftheconcentrationofiaaandsomeofitsconjugatesduringtheinductionofsomaticembryogenesisincoffeacanephora |
| _version_ |
1756089367267377152 |