Influence of molecular weight and grafting density of PEG on the surface properties of polyurethanes and their effect on the viability and morphology of fibroblasts and osteoblasts
Grafting polyethylene glycol (PEG) onto a polymer’s surface is widely used to improve biocompatibility by reducing protein and cell adhesion. Although PEG is considered to be bioinert, its incorporation onto biomaterials has shown to improve cell viability depending on the amount and molecular weight (MW) used. This phenomenon was studied here by grafting PEG of three MW onto polyurethane (PU) substrata at three molar concentrations to assess their effect on PU surface properties and on the viability of osteoblasts and fibroblasts. PEG formed a covering on the substrata which increased the hydrophilicity and surface energy of PUs. Among the results, it was observed that osteoblast viability increased for all MW and grafting densities of PEG employed compared with unmodified PU. However, fibroblast viability only increased at certain combinations of MW and grafting densities of PEG, suggesting an optimal level of these parameters. PEG grafting also promoted a more spread cell morphology than that exhibited by unmodified PU; nevertheless, cells became apoptotic-like as PEG MW and grafting density were increased. These effects on cells could be due to PEG affecting culture medium pH, which became more alkaline at higher MW and concentrations of PEG. Results support the hypothesis that surface energy of PU substrates can be tuned by controlling the MW and grafting density of PEG, but these parameters should be optimized to promote cell viability without inducing apoptotic-like behavior. © 2022 by the authors.
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Subjects: | info:eu-repo/classification/Autores/PEG, info:eu-repo/classification/Autores/VIABILITY, info:eu-repo/classification/Autores/OSTEOBLASTS, info:eu-repo/classification/Autores/FIBROBLASTS, info:eu-repo/classification/Autores/PH, info:eu-repo/classification/Autores/POLYURETHANE, info:eu-repo/classification/Autores/POLYETHYLENE GLYCOL, info:eu-repo/classification/cti/7, info:eu-repo/classification/cti/33, info:eu-repo/classification/cti/3312, info:eu-repo/classification/cti/331208, |
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dig-cicy-1003-28202023-10-26T22:36:22Z Influence of molecular weight and grafting density of PEG on the surface properties of polyurethanes and their effect on the viability and morphology of fibroblasts and osteoblasts ANTONIO DAVID ABREU REJON WILBERTH ANTONIO HERRERA KAO ALEJANDRO MAY PAT Alejandro Avila-Ortega NAYELI RODRIGUEZ FUENTES Jorge Alonso Uribe Calderón JOSE MANUEL CERVANTES UC 2022 info:eu-repo/semantics/article Grafting polyethylene glycol (PEG) onto a polymer’s surface is widely used to improve biocompatibility by reducing protein and cell adhesion. Although PEG is considered to be bioinert, its incorporation onto biomaterials has shown to improve cell viability depending on the amount and molecular weight (MW) used. This phenomenon was studied here by grafting PEG of three MW onto polyurethane (PU) substrata at three molar concentrations to assess their effect on PU surface properties and on the viability of osteoblasts and fibroblasts. PEG formed a covering on the substrata which increased the hydrophilicity and surface energy of PUs. Among the results, it was observed that osteoblast viability increased for all MW and grafting densities of PEG employed compared with unmodified PU. However, fibroblast viability only increased at certain combinations of MW and grafting densities of PEG, suggesting an optimal level of these parameters. PEG grafting also promoted a more spread cell morphology than that exhibited by unmodified PU; nevertheless, cells became apoptotic-like as PEG MW and grafting density were increased. These effects on cells could be due to PEG affecting culture medium pH, which became more alkaline at higher MW and concentrations of PEG. Results support the hypothesis that surface energy of PU substrates can be tuned by controlling the MW and grafting density of PEG, but these parameters should be optimized to promote cell viability without inducing apoptotic-like behavior. © 2022 by the authors. info:eu-repo/classification/Autores/PEG info:eu-repo/classification/Autores/VIABILITY info:eu-repo/classification/Autores/OSTEOBLASTS info:eu-repo/classification/Autores/FIBROBLASTS info:eu-repo/classification/Autores/PH info:eu-repo/classification/Autores/POLYURETHANE info:eu-repo/classification/Autores/POLYETHYLENE GLYCOL info:eu-repo/classification/cti/7 info:eu-repo/classification/cti/33 info:eu-repo/classification/cti/3312 info:eu-repo/classification/cti/331208 info:eu-repo/classification/cti/331208 Polymers, 14(22), 4912, 2022. http://cicy.repositorioinstitucional.mx/jspui/handle/1003/2820 info:eu-repo/semantics/datasetDOI/https://doi.org/10.3390/polym14224912 info:eu-repo/semantics/openAccess eng citation:Abreu-Rejón, A.D.; Herrera-Kao, W.A.; May-Pat, A.; Ávila-Ortega, A.; Rodríguez-Fuentes, N.; Uribe-Calderón, J.A.; Cervantes-Uc, J.M. Influence of Molecular Weight and Grafting Density of PEG on the Surface Properties of Polyurethanes and Their Effect on the Viability and Morphology of Fibroblasts and Osteoblasts. Polymers 2022, 14, 4912. https://doi.org/10.3390/ polym14224912 http://creativecommons.org/licenses/by-nc-nd/4.0 info:eu-repo/semantics/publishedVersion application/pdf |
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info:eu-repo/classification/Autores/PEG info:eu-repo/classification/Autores/VIABILITY info:eu-repo/classification/Autores/OSTEOBLASTS info:eu-repo/classification/Autores/FIBROBLASTS info:eu-repo/classification/Autores/PH info:eu-repo/classification/Autores/POLYURETHANE info:eu-repo/classification/Autores/POLYETHYLENE GLYCOL info:eu-repo/classification/cti/7 info:eu-repo/classification/cti/33 info:eu-repo/classification/cti/3312 info:eu-repo/classification/cti/331208 info:eu-repo/classification/cti/331208 info:eu-repo/classification/Autores/PEG info:eu-repo/classification/Autores/VIABILITY info:eu-repo/classification/Autores/OSTEOBLASTS info:eu-repo/classification/Autores/FIBROBLASTS info:eu-repo/classification/Autores/PH info:eu-repo/classification/Autores/POLYURETHANE info:eu-repo/classification/Autores/POLYETHYLENE GLYCOL info:eu-repo/classification/cti/7 info:eu-repo/classification/cti/33 info:eu-repo/classification/cti/3312 info:eu-repo/classification/cti/331208 info:eu-repo/classification/cti/331208 |
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info:eu-repo/classification/Autores/PEG info:eu-repo/classification/Autores/VIABILITY info:eu-repo/classification/Autores/OSTEOBLASTS info:eu-repo/classification/Autores/FIBROBLASTS info:eu-repo/classification/Autores/PH info:eu-repo/classification/Autores/POLYURETHANE info:eu-repo/classification/Autores/POLYETHYLENE GLYCOL info:eu-repo/classification/cti/7 info:eu-repo/classification/cti/33 info:eu-repo/classification/cti/3312 info:eu-repo/classification/cti/331208 info:eu-repo/classification/cti/331208 info:eu-repo/classification/Autores/PEG info:eu-repo/classification/Autores/VIABILITY info:eu-repo/classification/Autores/OSTEOBLASTS info:eu-repo/classification/Autores/FIBROBLASTS info:eu-repo/classification/Autores/PH info:eu-repo/classification/Autores/POLYURETHANE info:eu-repo/classification/Autores/POLYETHYLENE GLYCOL info:eu-repo/classification/cti/7 info:eu-repo/classification/cti/33 info:eu-repo/classification/cti/3312 info:eu-repo/classification/cti/331208 info:eu-repo/classification/cti/331208 ANTONIO DAVID ABREU REJON WILBERTH ANTONIO HERRERA KAO ALEJANDRO MAY PAT Alejandro Avila-Ortega NAYELI RODRIGUEZ FUENTES Jorge Alonso Uribe Calderón JOSE MANUEL CERVANTES UC Influence of molecular weight and grafting density of PEG on the surface properties of polyurethanes and their effect on the viability and morphology of fibroblasts and osteoblasts |
description |
Grafting polyethylene glycol (PEG) onto a polymer’s surface is widely used to improve biocompatibility by reducing protein and cell adhesion. Although PEG is considered to be bioinert, its incorporation onto biomaterials has shown to improve cell viability depending on the amount and molecular weight (MW) used. This phenomenon was studied here by grafting PEG of three MW onto polyurethane (PU) substrata at three molar concentrations to assess their effect on PU surface properties and on the viability of osteoblasts and fibroblasts. PEG formed a covering on the substrata which increased the hydrophilicity and surface energy of PUs. Among the results, it was observed that osteoblast viability increased for all MW and grafting densities of PEG employed compared with unmodified PU. However, fibroblast viability only increased at certain combinations of MW and grafting densities of PEG, suggesting an optimal level of these parameters. PEG grafting also promoted a more spread cell morphology than that exhibited by unmodified PU; nevertheless, cells became apoptotic-like as PEG MW and grafting density were increased. These effects on cells could be due to PEG affecting culture medium pH, which became more alkaline at higher MW and concentrations of PEG. Results support the hypothesis that surface energy of PU substrates can be tuned by controlling the MW and grafting density of PEG, but these parameters should be optimized to promote cell viability without inducing apoptotic-like behavior. © 2022 by the authors. |
format |
info:eu-repo/semantics/article |
topic_facet |
info:eu-repo/classification/Autores/PEG info:eu-repo/classification/Autores/VIABILITY info:eu-repo/classification/Autores/OSTEOBLASTS info:eu-repo/classification/Autores/FIBROBLASTS info:eu-repo/classification/Autores/PH info:eu-repo/classification/Autores/POLYURETHANE info:eu-repo/classification/Autores/POLYETHYLENE GLYCOL info:eu-repo/classification/cti/7 info:eu-repo/classification/cti/33 info:eu-repo/classification/cti/3312 info:eu-repo/classification/cti/331208 info:eu-repo/classification/cti/331208 |
author |
ANTONIO DAVID ABREU REJON WILBERTH ANTONIO HERRERA KAO ALEJANDRO MAY PAT Alejandro Avila-Ortega NAYELI RODRIGUEZ FUENTES Jorge Alonso Uribe Calderón JOSE MANUEL CERVANTES UC |
author_facet |
ANTONIO DAVID ABREU REJON WILBERTH ANTONIO HERRERA KAO ALEJANDRO MAY PAT Alejandro Avila-Ortega NAYELI RODRIGUEZ FUENTES Jorge Alonso Uribe Calderón JOSE MANUEL CERVANTES UC |
author_sort |
ANTONIO DAVID ABREU REJON |
title |
Influence of molecular weight and grafting density of PEG on the surface properties of polyurethanes and their effect on the viability and morphology of fibroblasts and osteoblasts |
title_short |
Influence of molecular weight and grafting density of PEG on the surface properties of polyurethanes and their effect on the viability and morphology of fibroblasts and osteoblasts |
title_full |
Influence of molecular weight and grafting density of PEG on the surface properties of polyurethanes and their effect on the viability and morphology of fibroblasts and osteoblasts |
title_fullStr |
Influence of molecular weight and grafting density of PEG on the surface properties of polyurethanes and their effect on the viability and morphology of fibroblasts and osteoblasts |
title_full_unstemmed |
Influence of molecular weight and grafting density of PEG on the surface properties of polyurethanes and their effect on the viability and morphology of fibroblasts and osteoblasts |
title_sort |
influence of molecular weight and grafting density of peg on the surface properties of polyurethanes and their effect on the viability and morphology of fibroblasts and osteoblasts |
url |
http://cicy.repositorioinstitucional.mx/jspui/handle/1003/2820 |
work_keys_str_mv |
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1781883621776293888 |