In vitro assessment of organic and residual fractions of nematicidal culture filtrates from thirteen tropical Trichoderma strains and metabolic profiles of most-active
The nematicidal properties of Trichoderma species have potential for developing safer biocontrol agents. In the present study, 13 native Trichoderma strains from T. citrinoviride, T. ghanense (2 strains), T. harzianum (4), T. koningiopsis, T. simmonsii, and T. virens (4) with nematicidal activity were selected and cultured in potato dextrose broth to obtain a culture filtrate (CF) for each. Each CF was partitioned with ethyl acetate to obtain organic (EA) and residual filtrate (RF) fractions, which were then tested on second-stage juveniles (J2s) of the nematodes Meloidogyne javanica and M. incognita in a microdilution assay. The most lethal strains were T. harzianum Th43-14, T. koningiopsis Th41-11, T. ghanense Th02-04, and T. virens Th32-09, which caused 51–100% mortality (%M) of J2s of both nematodes, mainly due to their RF fractions. Liquid chromatography–diode array detector-electrospray-high resolution mass spectrometry analysis of the most-active fractions revealed sesquiterpene and polyketide-like metabolites produced by the four active strains. These native Trichoderma strains have a high potential to develop safer natural products for the biocontrol of Meloidogyne species. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
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Subjects: | info:eu-repo/classification/Autores/ETHYL ACETATE FRACTION, info:eu-repo/classification/Autores/HRMS DEREPLICATION, info:eu-repo/classification/Autores/TRICHODERMA GHANENSE, info:eu-repo/classification/Autores/TRICHODERMA HARZIANUM, info:eu-repo/classification/Autores/TRICHODERMA KONINGIOPSIS, info:eu-repo/classification/Autores/TRICHODERMA VIRENS, info:eu-repo/classification/cti/2, info:eu-repo/classification/cti/24, info:eu-repo/classification/cti/2403, info:eu-repo/classification/cti/230221, |
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dig-cicy-1003-28172023-10-24T22:32:51Z In vitro assessment of organic and residual fractions of nematicidal culture filtrates from thirteen tropical Trichoderma strains and metabolic profiles of most-active FELICIA AMALIA MOO KOH JAIRO CRISTOBAL ALEJO MARIA FE ANDRES Jesús M. Martín Serrano Fernando Reyes JOSE TUN MARIA MARCELA GAMBOA ANGULO 2022 info:eu-repo/semantics/article The nematicidal properties of Trichoderma species have potential for developing safer biocontrol agents. In the present study, 13 native Trichoderma strains from T. citrinoviride, T. ghanense (2 strains), T. harzianum (4), T. koningiopsis, T. simmonsii, and T. virens (4) with nematicidal activity were selected and cultured in potato dextrose broth to obtain a culture filtrate (CF) for each. Each CF was partitioned with ethyl acetate to obtain organic (EA) and residual filtrate (RF) fractions, which were then tested on second-stage juveniles (J2s) of the nematodes Meloidogyne javanica and M. incognita in a microdilution assay. The most lethal strains were T. harzianum Th43-14, T. koningiopsis Th41-11, T. ghanense Th02-04, and T. virens Th32-09, which caused 51–100% mortality (%M) of J2s of both nematodes, mainly due to their RF fractions. Liquid chromatography–diode array detector-electrospray-high resolution mass spectrometry analysis of the most-active fractions revealed sesquiterpene and polyketide-like metabolites produced by the four active strains. These native Trichoderma strains have a high potential to develop safer natural products for the biocontrol of Meloidogyne species. © 2022 by the authors. Licensee MDPI, Basel, Switzerland. info:eu-repo/classification/Autores/ETHYL ACETATE FRACTION info:eu-repo/classification/Autores/HRMS DEREPLICATION info:eu-repo/classification/Autores/TRICHODERMA GHANENSE info:eu-repo/classification/Autores/TRICHODERMA HARZIANUM info:eu-repo/classification/Autores/TRICHODERMA KONINGIOPSIS info:eu-repo/classification/Autores/TRICHODERMA VIRENS info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/24 info:eu-repo/classification/cti/2403 info:eu-repo/classification/cti/230221 info:eu-repo/classification/cti/230221 Journal of Fungi, 8(1), 82, 2022. http://cicy.repositorioinstitucional.mx/jspui/handle/1003/2817 info:eu-repo/semantics/datasetDOI/https://doi.org/10.3390/jof8010082 info:eu-repo/semantics/openAccess eng citation:Moo-Koh, F.A.; Cristóbal-Alejo, J.; Andrés, M.F.; Martín, J.; Reyes, F.; Tun-Suárez, J.M.; Gamboa-Angulo, M. In Vitro Assessment of Organic and Residual Fractions of Nematicidal Culture Filtrates from Thirteen Tropical Trichoderma Strains and Metabolic Profiles of Most-Active. J. Fungi 2022, 8, 82.https://doi.org/10.3390/ jof8010082 http://creativecommons.org/licenses/by-nc-nd/4.0 info:eu-repo/semantics/publishedVersion application/pdf |
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info:eu-repo/classification/Autores/ETHYL ACETATE FRACTION info:eu-repo/classification/Autores/HRMS DEREPLICATION info:eu-repo/classification/Autores/TRICHODERMA GHANENSE info:eu-repo/classification/Autores/TRICHODERMA HARZIANUM info:eu-repo/classification/Autores/TRICHODERMA KONINGIOPSIS info:eu-repo/classification/Autores/TRICHODERMA VIRENS info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/24 info:eu-repo/classification/cti/2403 info:eu-repo/classification/cti/230221 info:eu-repo/classification/cti/230221 info:eu-repo/classification/Autores/ETHYL ACETATE FRACTION info:eu-repo/classification/Autores/HRMS DEREPLICATION info:eu-repo/classification/Autores/TRICHODERMA GHANENSE info:eu-repo/classification/Autores/TRICHODERMA HARZIANUM info:eu-repo/classification/Autores/TRICHODERMA KONINGIOPSIS info:eu-repo/classification/Autores/TRICHODERMA VIRENS info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/24 info:eu-repo/classification/cti/2403 info:eu-repo/classification/cti/230221 info:eu-repo/classification/cti/230221 |
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info:eu-repo/classification/Autores/ETHYL ACETATE FRACTION info:eu-repo/classification/Autores/HRMS DEREPLICATION info:eu-repo/classification/Autores/TRICHODERMA GHANENSE info:eu-repo/classification/Autores/TRICHODERMA HARZIANUM info:eu-repo/classification/Autores/TRICHODERMA KONINGIOPSIS info:eu-repo/classification/Autores/TRICHODERMA VIRENS info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/24 info:eu-repo/classification/cti/2403 info:eu-repo/classification/cti/230221 info:eu-repo/classification/cti/230221 info:eu-repo/classification/Autores/ETHYL ACETATE FRACTION info:eu-repo/classification/Autores/HRMS DEREPLICATION info:eu-repo/classification/Autores/TRICHODERMA GHANENSE info:eu-repo/classification/Autores/TRICHODERMA HARZIANUM info:eu-repo/classification/Autores/TRICHODERMA KONINGIOPSIS info:eu-repo/classification/Autores/TRICHODERMA VIRENS info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/24 info:eu-repo/classification/cti/2403 info:eu-repo/classification/cti/230221 info:eu-repo/classification/cti/230221 FELICIA AMALIA MOO KOH JAIRO CRISTOBAL ALEJO MARIA FE ANDRES Jesús M. Martín Serrano Fernando Reyes JOSE TUN MARIA MARCELA GAMBOA ANGULO In vitro assessment of organic and residual fractions of nematicidal culture filtrates from thirteen tropical Trichoderma strains and metabolic profiles of most-active |
description |
The nematicidal properties of Trichoderma species have potential for developing safer biocontrol agents. In the present study, 13 native Trichoderma strains from T. citrinoviride, T. ghanense (2 strains), T. harzianum (4), T. koningiopsis, T. simmonsii, and T. virens (4) with nematicidal activity were selected and cultured in potato dextrose broth to obtain a culture filtrate (CF) for each. Each CF was partitioned with ethyl acetate to obtain organic (EA) and residual filtrate (RF) fractions, which were then tested on second-stage juveniles (J2s) of the nematodes Meloidogyne javanica and M. incognita in a microdilution assay. The most lethal strains were T. harzianum Th43-14, T. koningiopsis Th41-11, T. ghanense Th02-04, and T. virens Th32-09, which caused 51–100% mortality (%M) of J2s of both nematodes, mainly due to their RF fractions. Liquid chromatography–diode array detector-electrospray-high resolution mass spectrometry analysis of the most-active fractions revealed sesquiterpene and polyketide-like metabolites produced by the four active strains. These native Trichoderma strains have a high potential to develop safer natural products for the biocontrol of Meloidogyne species. © 2022 by the authors. Licensee MDPI, Basel, Switzerland. |
format |
info:eu-repo/semantics/article |
topic_facet |
info:eu-repo/classification/Autores/ETHYL ACETATE FRACTION info:eu-repo/classification/Autores/HRMS DEREPLICATION info:eu-repo/classification/Autores/TRICHODERMA GHANENSE info:eu-repo/classification/Autores/TRICHODERMA HARZIANUM info:eu-repo/classification/Autores/TRICHODERMA KONINGIOPSIS info:eu-repo/classification/Autores/TRICHODERMA VIRENS info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/24 info:eu-repo/classification/cti/2403 info:eu-repo/classification/cti/230221 info:eu-repo/classification/cti/230221 |
author |
FELICIA AMALIA MOO KOH JAIRO CRISTOBAL ALEJO MARIA FE ANDRES Jesús M. Martín Serrano Fernando Reyes JOSE TUN MARIA MARCELA GAMBOA ANGULO |
author_facet |
FELICIA AMALIA MOO KOH JAIRO CRISTOBAL ALEJO MARIA FE ANDRES Jesús M. Martín Serrano Fernando Reyes JOSE TUN MARIA MARCELA GAMBOA ANGULO |
author_sort |
FELICIA AMALIA MOO KOH |
title |
In vitro assessment of organic and residual fractions of nematicidal culture filtrates from thirteen tropical Trichoderma strains and metabolic profiles of most-active |
title_short |
In vitro assessment of organic and residual fractions of nematicidal culture filtrates from thirteen tropical Trichoderma strains and metabolic profiles of most-active |
title_full |
In vitro assessment of organic and residual fractions of nematicidal culture filtrates from thirteen tropical Trichoderma strains and metabolic profiles of most-active |
title_fullStr |
In vitro assessment of organic and residual fractions of nematicidal culture filtrates from thirteen tropical Trichoderma strains and metabolic profiles of most-active |
title_full_unstemmed |
In vitro assessment of organic and residual fractions of nematicidal culture filtrates from thirteen tropical Trichoderma strains and metabolic profiles of most-active |
title_sort |
in vitro assessment of organic and residual fractions of nematicidal culture filtrates from thirteen tropical trichoderma strains and metabolic profiles of most-active |
url |
http://cicy.repositorioinstitucional.mx/jspui/handle/1003/2817 |
work_keys_str_mv |
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