Use of a simple method to isolate intact RNA from partially hydrated Selaginella Lepidophylla plants

Isolation of high-quality RNA is a necessary step in gene expression analysis. Although many methods can be used to isolate RNA from plants where contamination of preparations with complex carbohydrates or phenolic compounds is a problem, the application of these methods toSelaginella lepidophylla tissues has failed to obtain good-quality RNA. Here we introduce 2 modifications to the method developed by Chomczynski and Sacchi (1987), generating a simple and rapid method that allows the isolation of intact RNA fromS. lepidophylla-dehydrated tissues. Although the introduced modifications are not new, their addition proved to be decisive for success in RNA isolation. Quality of the RNA obtained was evaluated by electrophoresis in agarose and by 3 different PCR-based techniques—RT-PCR, RNA differential display, and synthesis of a cDNA library.

Bibliographic Details

Main Authors:	JUAN PABLO VALENZUELA AVENDAÑO, Iván Alfredo Estrada Mota, Gabriel Lizama Uc, RAMON ARMANDO SOUZA PERERA, Elisa Miriam Valenzuela Soto, José Juan Zúñiga Aguilar
Format:	info:eu-repo/semantics/article biblioteca
Language:	eng
Subjects:	info:eu-repo/classification/Autores/RESURRECTION PLANTS , info:eu-repo/classification/Autores/REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION , info:eu-repo/classification/Autores/RNA EXTRACTION, info:eu-repo/classification/cti/2, info:eu-repo/classification/cti/24, info:eu-repo/classification/cti/2415, info:eu-repo/classification/cti/241502,
Online Access:	http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1601
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