Transcriptomics and co-expression networks reveal tissue-specific responses and regulatory hubs under mild and severe drought in papaya (Carica papaya L.)
Plants respond to drought stress through the ABA dependent and independent pathways, which in turn modulate transcriptional regulatory hubs. Here, we employed Illumina RNA-Seq to analyze a total of 18 cDNA libraries from leaves, sap, and roots of papaya plants under drought stress. Reference and de novo transcriptomic analyses identified 8,549 and 6,089 drought-responsive genes and unigenes, respectively. Core sets of 6 and 34 genes were simultaneously up- or down-regulated, respectively, in all stressed samples. Moreover, GO enrichment analysis revealed that under moderate drought stress, processes related to cell cycle and DNA repair were up-regulated in leaves and sap; while responses to abiotic stress, hormone signaling, sucrose metabolism, and suberin biosynthesis were up-regulated in roots. Under severe drought stress, biological processes related to abiotic stress, hormone signaling, and oxidation-reduction were up-regulated in all tissues. Moreover, similar biological processes were commonly down-regulated in all stressed samples. Furthermore, co-expression network analysis revealed three and eight transcriptionally regulated modules in leaves and roots, respectively. Seventeen stress-related TFs were identified, potentially serving as main regulatory hubs in leaves and roots. Our findings provide insight into the molecular responses of papaya plant to drought, which could contribute to the improvement of this important tropical crop.
Main Authors: | , , , , , , , , |
---|---|
Format: | info:eu-repo/semantics/article biblioteca |
Language: | eng |
Subjects: | info:eu-repo/classification/Autores/PAPAYA, info:eu-repo/classification/Autores/CARICA PAPAYA, info:eu-repo/classification/cti/2, |
Online Access: | http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1562 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cicy-1003-1562 |
---|---|
record_format |
koha |
spelling |
dig-cicy-1003-15622019-06-05T14:13:19Z Transcriptomics and co-expression networks reveal tissue-specific responses and regulatory hubs under mild and severe drought in papaya (Carica papaya L.) SAMUEL DAVID GAMBOA TUZ ALEJANDRO PEREIRA SANTANA Jesús Alejandro Zamora Briseño Enrique Castaño de la Serna FRANCISCO LEONEL ESPADAS Y GIL JORGE TONATIUH AYALA SUMUANO MIGUEL ANGEL KEB LLANES Lorenzo Felipe Sánchez Teyer Luis Carlos Rodríguez Zapata 2018 info:eu-repo/semantics/article Plants respond to drought stress through the ABA dependent and independent pathways, which in turn modulate transcriptional regulatory hubs. Here, we employed Illumina RNA-Seq to analyze a total of 18 cDNA libraries from leaves, sap, and roots of papaya plants under drought stress. Reference and de novo transcriptomic analyses identified 8,549 and 6,089 drought-responsive genes and unigenes, respectively. Core sets of 6 and 34 genes were simultaneously up- or down-regulated, respectively, in all stressed samples. Moreover, GO enrichment analysis revealed that under moderate drought stress, processes related to cell cycle and DNA repair were up-regulated in leaves and sap; while responses to abiotic stress, hormone signaling, sucrose metabolism, and suberin biosynthesis were up-regulated in roots. Under severe drought stress, biological processes related to abiotic stress, hormone signaling, and oxidation-reduction were up-regulated in all tissues. Moreover, similar biological processes were commonly down-regulated in all stressed samples. Furthermore, co-expression network analysis revealed three and eight transcriptionally regulated modules in leaves and roots, respectively. Seventeen stress-related TFs were identified, potentially serving as main regulatory hubs in leaves and roots. Our findings provide insight into the molecular responses of papaya plant to drought, which could contribute to the improvement of this important tropical crop. info:eu-repo/classification/Autores/PAPAYA info:eu-repo/classification/Autores/CARICA PAPAYA info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 Scientific reports, 8(1), 14539, 2018. http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1562 info:eu-repo/semantics/datasetDOI/10.1038/s41598-018-32904-2 info:eu-repo/semantics/openAccess eng citation:Gamboa-Tuz, S. D., Pereira-Santana, A., Zamora-Briseño, J. A., Castano, E., Espadas-Gil, F., Ayala-Sumuano, J. T., ... & Rodríguez-Zapata, L. C. (2018). Transcriptomics and co-expression networks reveal tissue-specific responses and regulatory hubs under mild and severe drought in papaya (Carica papaya L.). Scientific reports, 8(1), 14539. http://creativecommons.org/licenses/by-nc-nd/4.0 info:eu-repo/semantics/publishedVersion application/pdf |
institution |
CICY |
collection |
DSpace |
country |
México |
countrycode |
MX |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cicy |
tag |
biblioteca |
region |
America del Norte |
libraryname |
Biblioteca del CICY |
language |
eng |
topic |
info:eu-repo/classification/Autores/PAPAYA info:eu-repo/classification/Autores/CARICA PAPAYA info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 info:eu-repo/classification/Autores/PAPAYA info:eu-repo/classification/Autores/CARICA PAPAYA info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 |
spellingShingle |
info:eu-repo/classification/Autores/PAPAYA info:eu-repo/classification/Autores/CARICA PAPAYA info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 info:eu-repo/classification/Autores/PAPAYA info:eu-repo/classification/Autores/CARICA PAPAYA info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 SAMUEL DAVID GAMBOA TUZ ALEJANDRO PEREIRA SANTANA Jesús Alejandro Zamora Briseño Enrique Castaño de la Serna FRANCISCO LEONEL ESPADAS Y GIL JORGE TONATIUH AYALA SUMUANO MIGUEL ANGEL KEB LLANES Lorenzo Felipe Sánchez Teyer Luis Carlos Rodríguez Zapata Transcriptomics and co-expression networks reveal tissue-specific responses and regulatory hubs under mild and severe drought in papaya (Carica papaya L.) |
description |
Plants respond to drought stress through the ABA dependent and independent pathways, which in turn modulate transcriptional regulatory hubs. Here, we employed Illumina RNA-Seq to analyze a total of 18 cDNA libraries from leaves, sap, and roots of papaya plants under drought stress. Reference and de novo transcriptomic analyses identified 8,549 and 6,089 drought-responsive genes and unigenes, respectively. Core sets of 6 and 34 genes were simultaneously up- or down-regulated, respectively, in all stressed samples. Moreover, GO enrichment analysis revealed that under moderate drought stress, processes related to cell cycle and DNA repair were up-regulated in leaves and sap; while responses to abiotic stress, hormone signaling, sucrose metabolism, and suberin biosynthesis were up-regulated in roots. Under severe drought stress, biological processes related to abiotic stress, hormone signaling, and oxidation-reduction were up-regulated in all tissues. Moreover, similar biological processes were commonly down-regulated in all stressed samples. Furthermore, co-expression network analysis revealed three and eight transcriptionally regulated modules in leaves and roots, respectively. Seventeen stress-related TFs were identified, potentially serving as main regulatory hubs in leaves and roots. Our findings provide insight into the molecular responses of papaya plant to drought, which could contribute to the improvement of this important tropical crop. |
format |
info:eu-repo/semantics/article |
topic_facet |
info:eu-repo/classification/Autores/PAPAYA info:eu-repo/classification/Autores/CARICA PAPAYA info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 |
author |
SAMUEL DAVID GAMBOA TUZ ALEJANDRO PEREIRA SANTANA Jesús Alejandro Zamora Briseño Enrique Castaño de la Serna FRANCISCO LEONEL ESPADAS Y GIL JORGE TONATIUH AYALA SUMUANO MIGUEL ANGEL KEB LLANES Lorenzo Felipe Sánchez Teyer Luis Carlos Rodríguez Zapata |
author_facet |
SAMUEL DAVID GAMBOA TUZ ALEJANDRO PEREIRA SANTANA Jesús Alejandro Zamora Briseño Enrique Castaño de la Serna FRANCISCO LEONEL ESPADAS Y GIL JORGE TONATIUH AYALA SUMUANO MIGUEL ANGEL KEB LLANES Lorenzo Felipe Sánchez Teyer Luis Carlos Rodríguez Zapata |
author_sort |
SAMUEL DAVID GAMBOA TUZ |
title |
Transcriptomics and co-expression networks reveal tissue-specific responses and regulatory hubs under mild and severe drought in papaya (Carica papaya L.) |
title_short |
Transcriptomics and co-expression networks reveal tissue-specific responses and regulatory hubs under mild and severe drought in papaya (Carica papaya L.) |
title_full |
Transcriptomics and co-expression networks reveal tissue-specific responses and regulatory hubs under mild and severe drought in papaya (Carica papaya L.) |
title_fullStr |
Transcriptomics and co-expression networks reveal tissue-specific responses and regulatory hubs under mild and severe drought in papaya (Carica papaya L.) |
title_full_unstemmed |
Transcriptomics and co-expression networks reveal tissue-specific responses and regulatory hubs under mild and severe drought in papaya (Carica papaya L.) |
title_sort |
transcriptomics and co-expression networks reveal tissue-specific responses and regulatory hubs under mild and severe drought in papaya (carica papaya l.) |
url |
http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1562 |
work_keys_str_mv |
AT samueldavidgamboatuz transcriptomicsandcoexpressionnetworksrevealtissuespecificresponsesandregulatoryhubsundermildandseveredroughtinpapayacaricapapayal AT alejandropereirasantana transcriptomicsandcoexpressionnetworksrevealtissuespecificresponsesandregulatoryhubsundermildandseveredroughtinpapayacaricapapayal AT jesusalejandrozamorabriseno transcriptomicsandcoexpressionnetworksrevealtissuespecificresponsesandregulatoryhubsundermildandseveredroughtinpapayacaricapapayal AT enriquecastanodelaserna transcriptomicsandcoexpressionnetworksrevealtissuespecificresponsesandregulatoryhubsundermildandseveredroughtinpapayacaricapapayal AT franciscoleonelespadasygil transcriptomicsandcoexpressionnetworksrevealtissuespecificresponsesandregulatoryhubsundermildandseveredroughtinpapayacaricapapayal AT jorgetonatiuhayalasumuano transcriptomicsandcoexpressionnetworksrevealtissuespecificresponsesandregulatoryhubsundermildandseveredroughtinpapayacaricapapayal AT miguelangelkebllanes transcriptomicsandcoexpressionnetworksrevealtissuespecificresponsesandregulatoryhubsundermildandseveredroughtinpapayacaricapapayal AT lorenzofelipesanchezteyer transcriptomicsandcoexpressionnetworksrevealtissuespecificresponsesandregulatoryhubsundermildandseveredroughtinpapayacaricapapayal AT luiscarlosrodriguezzapata transcriptomicsandcoexpressionnetworksrevealtissuespecificresponsesandregulatoryhubsundermildandseveredroughtinpapayacaricapapayal |
_version_ |
1756089264885465088 |