Transcriptomics and co-expression networks reveal tissue-specific responses and regulatory hubs under mild and severe drought in papaya (Carica papaya L.)

SAMUEL DAVID GAMBOA TUZ; ALEJANDRO PEREIRA SANTANA; Jesús Alejandro Zamora Briseño; Enrique Castaño de la Serna; FRANCISCO LEONEL ESPADAS Y GIL; JORGE TONATIUH AYALA SUMUANO; MIGUEL ANGEL KEB LLANES; Lorenzo Felipe Sánchez Teyer; Luis Carlos Rodríguez Zapata

Transcriptomics and co-expression networks reveal tissue-specific responses and regulatory hubs under mild and severe drought in papaya (Carica papaya L.)

Plants respond to drought stress through the ABA dependent and independent pathways, which in turn modulate transcriptional regulatory hubs. Here, we employed Illumina RNA-Seq to analyze a total of 18 cDNA libraries from leaves, sap, and roots of papaya plants under drought stress. Reference and de novo transcriptomic analyses identified 8,549 and 6,089 drought-responsive genes and unigenes, respectively. Core sets of 6 and 34 genes were simultaneously up- or down-regulated, respectively, in all stressed samples. Moreover, GO enrichment analysis revealed that under moderate drought stress, processes related to cell cycle and DNA repair were up-regulated in leaves and sap; while responses to abiotic stress, hormone signaling, sucrose metabolism, and suberin biosynthesis were up-regulated in roots. Under severe drought stress, biological processes related to abiotic stress, hormone signaling, and oxidation-reduction were up-regulated in all tissues. Moreover, similar biological processes were commonly down-regulated in all stressed samples. Furthermore, co-expression network analysis revealed three and eight transcriptionally regulated modules in leaves and roots, respectively. Seventeen stress-related TFs were identified, potentially serving as main regulatory hubs in leaves and roots. Our findings provide insight into the molecular responses of papaya plant to drought, which could contribute to the improvement of this important tropical crop.

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Main Authors:	SAMUEL DAVID GAMBOA TUZ, ALEJANDRO PEREIRA SANTANA, Jesús Alejandro Zamora Briseño, Enrique Castaño de la Serna, FRANCISCO LEONEL ESPADAS Y GIL, JORGE TONATIUH AYALA SUMUANO, MIGUEL ANGEL KEB LLANES, Lorenzo Felipe Sánchez Teyer, Luis Carlos Rodríguez Zapata
Format:	info:eu-repo/semantics/article biblioteca
Language:	eng
Subjects:	info:eu-repo/classification/Autores/PAPAYA, info:eu-repo/classification/Autores/CARICA PAPAYA, info:eu-repo/classification/cti/2,
Online Access:	http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1562
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spelling	dig-cicy-1003-15622019-06-05T14:13:19Z Transcriptomics and co-expression networks reveal tissue-specific responses and regulatory hubs under mild and severe drought in papaya (Carica papaya L.) SAMUEL DAVID GAMBOA TUZ ALEJANDRO PEREIRA SANTANA Jesús Alejandro Zamora Briseño Enrique Castaño de la Serna FRANCISCO LEONEL ESPADAS Y GIL JORGE TONATIUH AYALA SUMUANO MIGUEL ANGEL KEB LLANES Lorenzo Felipe Sánchez Teyer Luis Carlos Rodríguez Zapata 2018 info:eu-repo/semantics/article Plants respond to drought stress through the ABA dependent and independent pathways, which in turn modulate transcriptional regulatory hubs. Here, we employed Illumina RNA-Seq to analyze a total of 18 cDNA libraries from leaves, sap, and roots of papaya plants under drought stress. Reference and de novo transcriptomic analyses identified 8,549 and 6,089 drought-responsive genes and unigenes, respectively. Core sets of 6 and 34 genes were simultaneously up- or down-regulated, respectively, in all stressed samples. Moreover, GO enrichment analysis revealed that under moderate drought stress, processes related to cell cycle and DNA repair were up-regulated in leaves and sap; while responses to abiotic stress, hormone signaling, sucrose metabolism, and suberin biosynthesis were up-regulated in roots. Under severe drought stress, biological processes related to abiotic stress, hormone signaling, and oxidation-reduction were up-regulated in all tissues. Moreover, similar biological processes were commonly down-regulated in all stressed samples. Furthermore, co-expression network analysis revealed three and eight transcriptionally regulated modules in leaves and roots, respectively. Seventeen stress-related TFs were identified, potentially serving as main regulatory hubs in leaves and roots. Our findings provide insight into the molecular responses of papaya plant to drought, which could contribute to the improvement of this important tropical crop. info:eu-repo/classification/Autores/PAPAYA info:eu-repo/classification/Autores/CARICA PAPAYA info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 Scientific reports, 8(1), 14539, 2018. http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1562 info:eu-repo/semantics/datasetDOI/10.1038/s41598-018-32904-2 info:eu-repo/semantics/openAccess eng citation:Gamboa-Tuz, S. D., Pereira-Santana, A., Zamora-Briseño, J. A., Castano, E., Espadas-Gil, F., Ayala-Sumuano, J. T., ... & Rodríguez-Zapata, L. C. (2018). Transcriptomics and co-expression networks reveal tissue-specific responses and regulatory hubs under mild and severe drought in papaya (Carica papaya L.). Scientific reports, 8(1), 14539. http://creativecommons.org/licenses/by-nc-nd/4.0 info:eu-repo/semantics/publishedVersion application/pdf
institution	CICY
collection	DSpace
country	México
countrycode	MX
component	Bibliográfico
access	En linea
databasecode	dig-cicy
tag	biblioteca
region	America del Norte
libraryname	Biblioteca del CICY
language	eng
topic	info:eu-repo/classification/Autores/PAPAYA info:eu-repo/classification/Autores/CARICA PAPAYA info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 info:eu-repo/classification/Autores/PAPAYA info:eu-repo/classification/Autores/CARICA PAPAYA info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2
spellingShingle	info:eu-repo/classification/Autores/PAPAYA info:eu-repo/classification/Autores/CARICA PAPAYA info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 info:eu-repo/classification/Autores/PAPAYA info:eu-repo/classification/Autores/CARICA PAPAYA info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 SAMUEL DAVID GAMBOA TUZ ALEJANDRO PEREIRA SANTANA Jesús Alejandro Zamora Briseño Enrique Castaño de la Serna FRANCISCO LEONEL ESPADAS Y GIL JORGE TONATIUH AYALA SUMUANO MIGUEL ANGEL KEB LLANES Lorenzo Felipe Sánchez Teyer Luis Carlos Rodríguez Zapata Transcriptomics and co-expression networks reveal tissue-specific responses and regulatory hubs under mild and severe drought in papaya (Carica papaya L.)
description	Plants respond to drought stress through the ABA dependent and independent pathways, which in turn modulate transcriptional regulatory hubs. Here, we employed Illumina RNA-Seq to analyze a total of 18 cDNA libraries from leaves, sap, and roots of papaya plants under drought stress. Reference and de novo transcriptomic analyses identified 8,549 and 6,089 drought-responsive genes and unigenes, respectively. Core sets of 6 and 34 genes were simultaneously up- or down-regulated, respectively, in all stressed samples. Moreover, GO enrichment analysis revealed that under moderate drought stress, processes related to cell cycle and DNA repair were up-regulated in leaves and sap; while responses to abiotic stress, hormone signaling, sucrose metabolism, and suberin biosynthesis were up-regulated in roots. Under severe drought stress, biological processes related to abiotic stress, hormone signaling, and oxidation-reduction were up-regulated in all tissues. Moreover, similar biological processes were commonly down-regulated in all stressed samples. Furthermore, co-expression network analysis revealed three and eight transcriptionally regulated modules in leaves and roots, respectively. Seventeen stress-related TFs were identified, potentially serving as main regulatory hubs in leaves and roots. Our findings provide insight into the molecular responses of papaya plant to drought, which could contribute to the improvement of this important tropical crop.
format	info:eu-repo/semantics/article
topic_facet	info:eu-repo/classification/Autores/PAPAYA info:eu-repo/classification/Autores/CARICA PAPAYA info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2
author	SAMUEL DAVID GAMBOA TUZ ALEJANDRO PEREIRA SANTANA Jesús Alejandro Zamora Briseño Enrique Castaño de la Serna FRANCISCO LEONEL ESPADAS Y GIL JORGE TONATIUH AYALA SUMUANO MIGUEL ANGEL KEB LLANES Lorenzo Felipe Sánchez Teyer Luis Carlos Rodríguez Zapata
author_facet	SAMUEL DAVID GAMBOA TUZ ALEJANDRO PEREIRA SANTANA Jesús Alejandro Zamora Briseño Enrique Castaño de la Serna FRANCISCO LEONEL ESPADAS Y GIL JORGE TONATIUH AYALA SUMUANO MIGUEL ANGEL KEB LLANES Lorenzo Felipe Sánchez Teyer Luis Carlos Rodríguez Zapata
author_sort	SAMUEL DAVID GAMBOA TUZ
title	Transcriptomics and co-expression networks reveal tissue-specific responses and regulatory hubs under mild and severe drought in papaya (Carica papaya L.)
title_short	Transcriptomics and co-expression networks reveal tissue-specific responses and regulatory hubs under mild and severe drought in papaya (Carica papaya L.)
title_full	Transcriptomics and co-expression networks reveal tissue-specific responses and regulatory hubs under mild and severe drought in papaya (Carica papaya L.)
title_fullStr	Transcriptomics and co-expression networks reveal tissue-specific responses and regulatory hubs under mild and severe drought in papaya (Carica papaya L.)
title_full_unstemmed	Transcriptomics and co-expression networks reveal tissue-specific responses and regulatory hubs under mild and severe drought in papaya (Carica papaya L.)
title_sort	transcriptomics and co-expression networks reveal tissue-specific responses and regulatory hubs under mild and severe drought in papaya (carica papaya l.)
url	http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1562
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Transcriptomics and co-expression networks reveal tissue-specific responses and regulatory hubs under mild and severe drought in papaya (Carica papaya L.)

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