Epitope selection for multipeptide proteins: the case of RNA polymerase IV and V

Epitope selection for multipeptide proteins: the case of RNA polymerase IV and V

Large multipeptide protein complexes have provided a challenge for epitope selection, which is required for immunological protocols where native conformations are needed. Immunolocalization requires native conformation of the proteins, which is essential for further understanding of biological activity. RNA polymerase IV and V are multisubunit proteins that interact with other factors in the RNA-directed DNA methylation pathway for control of DNA silencing by small interfering RNA and DNA methylation. DNA silencing is an important process during cell differentiation, nuclear structure and viral control among others. RNA polymerase IV and V are yet to be studied in model monocot systems like Oryza sativa, which may provide further data to define if the genetic silencing mechanism has diverged over time as compared to dicots. Here we show anin silicoselection process of exposed sequences and their use for obtaining antibodies against native RNA polymerase IV and V inO. sativa.

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Main Author: ENRIQUE CASTAÑO DE LA SERNA
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Online Access:http://cicy.repositorioinstitucional.mx/jspui/handle/1003/151
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spelling dig-cicy-1003-1512016-10-25T14:28:24Z Epitope selection for multipeptide proteins: the case of RNA polymerase IV and V ENRIQUE CASTAÑO DE LA SERNA 2012-07-29 info:eu-repo/semantics/article Large multipeptide protein complexes have provided a challenge for epitope selection, which is required for immunological protocols where native conformations are needed. Immunolocalization requires native conformation of the proteins, which is essential for further understanding of biological activity. RNA polymerase IV and V are multisubunit proteins that interact with other factors in the RNA-directed DNA methylation pathway for control of DNA silencing by small interfering RNA and DNA methylation. DNA silencing is an important process during cell differentiation, nuclear structure and viral control among others. RNA polymerase IV and V are yet to be studied in model monocot systems like Oryza sativa, which may provide further data to define if the genetic silencing mechanism has diverged over time as compared to dicots. Here we show anin silicoselection process of exposed sequences and their use for obtaining antibodies against native RNA polymerase IV and V inO. sativa. info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 http://cicy.repositorioinstitucional.mx/jspui/handle/1003/151 info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0 application/pdf
institution CICY
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country México
countrycode MX
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libraryname Biblioteca del CICY
topic info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/2
spellingShingle info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/2
ENRIQUE CASTAÑO DE LA SERNA
Epitope selection for multipeptide proteins: the case of RNA polymerase IV and V
description Large multipeptide protein complexes have provided a challenge for epitope selection, which is required for immunological protocols where native conformations are needed. Immunolocalization requires native conformation of the proteins, which is essential for further understanding of biological activity. RNA polymerase IV and V are multisubunit proteins that interact with other factors in the RNA-directed DNA methylation pathway for control of DNA silencing by small interfering RNA and DNA methylation. DNA silencing is an important process during cell differentiation, nuclear structure and viral control among others. RNA polymerase IV and V are yet to be studied in model monocot systems like Oryza sativa, which may provide further data to define if the genetic silencing mechanism has diverged over time as compared to dicots. Here we show anin silicoselection process of exposed sequences and their use for obtaining antibodies against native RNA polymerase IV and V inO. sativa.
format info:eu-repo/semantics/article
topic_facet info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/2
author ENRIQUE CASTAÑO DE LA SERNA
author_facet ENRIQUE CASTAÑO DE LA SERNA
author_sort ENRIQUE CASTAÑO DE LA SERNA
title Epitope selection for multipeptide proteins: the case of RNA polymerase IV and V
title_short Epitope selection for multipeptide proteins: the case of RNA polymerase IV and V
title_full Epitope selection for multipeptide proteins: the case of RNA polymerase IV and V
title_fullStr Epitope selection for multipeptide proteins: the case of RNA polymerase IV and V
title_full_unstemmed Epitope selection for multipeptide proteins: the case of RNA polymerase IV and V
title_sort epitope selection for multipeptide proteins: the case of rna polymerase iv and v
url http://cicy.repositorioinstitucional.mx/jspui/handle/1003/151
work_keys_str_mv AT enriquecastanodelaserna epitopeselectionformultipeptideproteinsthecaseofrnapolymeraseivandv
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