Expression of RNA polymerase IV and V in Oryza sativa

RNA polymerase IV and V are principal players in the RdDM pathway, where their current study has shown interaction of several factors that control DNA silencing of intergenic regions and siRNA production. DNA silencing is an important process during cell differentiation, nuclear structure and viral control. However, RNA pol IV and V are yet to be study in model monocot systems like Oryza sativa that can provide further information on genetic silence mechanism in plats. We show the expression pattern of these polymerases in tissues extracts of Oryza sativa. Detectable amounts of these polymerases are found in specific adult plant tissues and particularly expressed during somatic embryogenesis but not during early stages of normal embryo development. The use of synthetic auxin leads to an induction of both RNA pol IV and V in scutellum tissue where nuclear localization may be required for genome reorganization and gene silencing.

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Main Author: ENRIQUE CASTAÑO DE LA SERNA
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Online Access:http://cicy.repositorioinstitucional.mx/jspui/handle/1003/149
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spelling dig-cicy-1003-1492016-10-25T13:55:01Z Expression of RNA polymerase IV and V in Oryza sativa ENRIQUE CASTAÑO DE LA SERNA 2012-03-15 info:eu-repo/semantics/article RNA polymerase IV and V are principal players in the RdDM pathway, where their current study has shown interaction of several factors that control DNA silencing of intergenic regions and siRNA production. DNA silencing is an important process during cell differentiation, nuclear structure and viral control. However, RNA pol IV and V are yet to be study in model monocot systems like Oryza sativa that can provide further information on genetic silence mechanism in plats. We show the expression pattern of these polymerases in tissues extracts of Oryza sativa. Detectable amounts of these polymerases are found in specific adult plant tissues and particularly expressed during somatic embryogenesis but not during early stages of normal embryo development. The use of synthetic auxin leads to an induction of both RNA pol IV and V in scutellum tissue where nuclear localization may be required for genome reorganization and gene silencing. info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 http://cicy.repositorioinstitucional.mx/jspui/handle/1003/149 info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0 application/pdf
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topic info:eu-repo/classification/cti/2
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ENRIQUE CASTAÑO DE LA SERNA
Expression of RNA polymerase IV and V in Oryza sativa
description RNA polymerase IV and V are principal players in the RdDM pathway, where their current study has shown interaction of several factors that control DNA silencing of intergenic regions and siRNA production. DNA silencing is an important process during cell differentiation, nuclear structure and viral control. However, RNA pol IV and V are yet to be study in model monocot systems like Oryza sativa that can provide further information on genetic silence mechanism in plats. We show the expression pattern of these polymerases in tissues extracts of Oryza sativa. Detectable amounts of these polymerases are found in specific adult plant tissues and particularly expressed during somatic embryogenesis but not during early stages of normal embryo development. The use of synthetic auxin leads to an induction of both RNA pol IV and V in scutellum tissue where nuclear localization may be required for genome reorganization and gene silencing.
format info:eu-repo/semantics/article
topic_facet info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/2
author ENRIQUE CASTAÑO DE LA SERNA
author_facet ENRIQUE CASTAÑO DE LA SERNA
author_sort ENRIQUE CASTAÑO DE LA SERNA
title Expression of RNA polymerase IV and V in Oryza sativa
title_short Expression of RNA polymerase IV and V in Oryza sativa
title_full Expression of RNA polymerase IV and V in Oryza sativa
title_fullStr Expression of RNA polymerase IV and V in Oryza sativa
title_full_unstemmed Expression of RNA polymerase IV and V in Oryza sativa
title_sort expression of rna polymerase iv and v in oryza sativa
url http://cicy.repositorioinstitucional.mx/jspui/handle/1003/149
work_keys_str_mv AT enriquecastanodelaserna expressionofrnapolymeraseivandvinoryzasativa
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