Agrobacterium tumefaciens-transient genetic transformation of Habanero pepper (Capsicum chinense Jacq.) leaf explants
Most of the pepper species of the genus Capsicum have been recalcitrant to efficient Agrobacterium tumefaciens-mediated stable or transient, genetic transformation. In the present work, we optimized a protocol for transient transformation of the Habanero pepper (Capsicum chinense Jacq.) through the standardization of several experimental factors. These included the age of the plants, the temperature, the length of co-cultivation, the application of a negative (vacuum) and/or a positive (infiltration) pressure, along with micro injection, the use of acetosyringone during the bacterial culturing, and modification of the pH during the GUS assay to eliminate the endogenous β-glucuronidase activity. The standardized protocol, which yielded nearly 55% fully transformed leaf explants, was used to successfully mobilize two empty binary vectors (pCAMBIA2301 and pCAMex), as well as the C. chinense cDNAs encoding the pathogenesis-related protein 10 and esterase, respectively.
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dig-cicy-1003-13182018-10-25T16:41:48Z Agrobacterium tumefaciens-transient genetic transformation of Habanero pepper (Capsicum chinense Jacq.) leaf explants GUADALUPE FABIOLA ARCOS ORTEGA RAFAEL ANTONIO CHAN KUUK WILMA ARACELY GONZALEZ KANTUN RAMON ARMANDO SOUZA PERERA YUMI ELENA NAKAZAWA UEJI ELIDE AVILES BERZUNZA GREGORIO DEL CARMEN GODOY HERNANDEZ Michael Lawton JOSE JUAN ZUÑIGA AGUILAR 2010 info:eu-repo/semantics/article Most of the pepper species of the genus Capsicum have been recalcitrant to efficient Agrobacterium tumefaciens-mediated stable or transient, genetic transformation. In the present work, we optimized a protocol for transient transformation of the Habanero pepper (Capsicum chinense Jacq.) through the standardization of several experimental factors. These included the age of the plants, the temperature, the length of co-cultivation, the application of a negative (vacuum) and/or a positive (infiltration) pressure, along with micro injection, the use of acetosyringone during the bacterial culturing, and modification of the pH during the GUS assay to eliminate the endogenous β-glucuronidase activity. The standardized protocol, which yielded nearly 55% fully transformed leaf explants, was used to successfully mobilize two empty binary vectors (pCAMBIA2301 and pCAMex), as well as the C. chinense cDNAs encoding the pathogenesis-related protein 10 and esterase, respectively. info:eu-repo/classification/Autores/COCULTIVATION info:eu-repo/classification/Autores/GUS ASSAY info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 Electronic Journal of Biotechnology, 13(4), 7-8, 2010. http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1318 info:eu-repo/semantics/datasetDOI/10.2225/vol13-issue4-fulltext-10 info:eu-repo/semantics/openAccess eng citation:Arcos-Ortega, G. F., Chan-Kuuk, R. A., González-Kantún, W. A., Souza-Perera, R., Nakazawa-Ueji, Y. E., Avilés-Berzunza, E., ... & Zúñiga Aguilar, J. J. (2010). Agrobacterium tumefaciens-transient genetic transformation of Habanero pepper (Capsicum chinense Jacq.) leaf explants. Electronic Journal of Biotechnology, 13(4), 7-8. http://creativecommons.org/licenses/by-nc-nd/4.0 info:eu-repo/semantics/publishedVersion application/pdf |
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info:eu-repo/classification/Autores/COCULTIVATION info:eu-repo/classification/Autores/GUS ASSAY info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 info:eu-repo/classification/Autores/COCULTIVATION info:eu-repo/classification/Autores/GUS ASSAY info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 |
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info:eu-repo/classification/Autores/COCULTIVATION info:eu-repo/classification/Autores/GUS ASSAY info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 info:eu-repo/classification/Autores/COCULTIVATION info:eu-repo/classification/Autores/GUS ASSAY info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 GUADALUPE FABIOLA ARCOS ORTEGA RAFAEL ANTONIO CHAN KUUK WILMA ARACELY GONZALEZ KANTUN RAMON ARMANDO SOUZA PERERA YUMI ELENA NAKAZAWA UEJI ELIDE AVILES BERZUNZA GREGORIO DEL CARMEN GODOY HERNANDEZ Michael Lawton JOSE JUAN ZUÑIGA AGUILAR Agrobacterium tumefaciens-transient genetic transformation of Habanero pepper (Capsicum chinense Jacq.) leaf explants |
description |
Most of the pepper species of the genus Capsicum have been recalcitrant to efficient Agrobacterium tumefaciens-mediated stable or transient, genetic transformation. In the present work, we optimized a protocol for transient transformation of the Habanero pepper (Capsicum chinense Jacq.) through the standardization of several experimental factors. These included the age of the plants, the temperature, the length of co-cultivation, the application of a negative (vacuum) and/or a positive (infiltration) pressure, along with micro injection, the use of acetosyringone during the bacterial culturing, and modification of the pH during the GUS assay to eliminate the endogenous β-glucuronidase activity. The standardized protocol, which yielded nearly 55% fully transformed leaf explants, was used to successfully mobilize two empty binary vectors (pCAMBIA2301 and pCAMex), as well as the C. chinense cDNAs encoding the pathogenesis-related protein 10 and esterase, respectively. |
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info:eu-repo/semantics/article |
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info:eu-repo/classification/Autores/COCULTIVATION info:eu-repo/classification/Autores/GUS ASSAY info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 |
author |
GUADALUPE FABIOLA ARCOS ORTEGA RAFAEL ANTONIO CHAN KUUK WILMA ARACELY GONZALEZ KANTUN RAMON ARMANDO SOUZA PERERA YUMI ELENA NAKAZAWA UEJI ELIDE AVILES BERZUNZA GREGORIO DEL CARMEN GODOY HERNANDEZ Michael Lawton JOSE JUAN ZUÑIGA AGUILAR |
author_facet |
GUADALUPE FABIOLA ARCOS ORTEGA RAFAEL ANTONIO CHAN KUUK WILMA ARACELY GONZALEZ KANTUN RAMON ARMANDO SOUZA PERERA YUMI ELENA NAKAZAWA UEJI ELIDE AVILES BERZUNZA GREGORIO DEL CARMEN GODOY HERNANDEZ Michael Lawton JOSE JUAN ZUÑIGA AGUILAR |
author_sort |
GUADALUPE FABIOLA ARCOS ORTEGA |
title |
Agrobacterium tumefaciens-transient genetic transformation of Habanero pepper (Capsicum chinense Jacq.) leaf explants |
title_short |
Agrobacterium tumefaciens-transient genetic transformation of Habanero pepper (Capsicum chinense Jacq.) leaf explants |
title_full |
Agrobacterium tumefaciens-transient genetic transformation of Habanero pepper (Capsicum chinense Jacq.) leaf explants |
title_fullStr |
Agrobacterium tumefaciens-transient genetic transformation of Habanero pepper (Capsicum chinense Jacq.) leaf explants |
title_full_unstemmed |
Agrobacterium tumefaciens-transient genetic transformation of Habanero pepper (Capsicum chinense Jacq.) leaf explants |
title_sort |
agrobacterium tumefaciens-transient genetic transformation of habanero pepper (capsicum chinense jacq.) leaf explants |
url |
http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1318 |
work_keys_str_mv |
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1756089229918601216 |