Nitrogen source and the effect of growth regulators on ammonium assimilation enzymes in tissue culture of Canavalia ensiformis (L.) DC
Ammonium assimilation enzymes in Canavalia ensiformis (L.) DC. calli respond differentially to the N source in the culture medium when subcultured under different hormonal regimes. With 1 mM NH,+ the aminative and deaminative activities of GDH were not affected by the auxin, so the observed changes were due to the different nitrate concentrations in the medium. When NH4 was inereased to 5 mM, there was a marked increase with NAA and a NO3 dose effect was noticed. GS activity was not affected by the ammonium concentration but it was affected by both nitrate and the auxin type, with NAA promoting higher activities
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Main Authors: | FELIPE AUGUSTO VAZQUEZ FLOTA, MARIA DE LOURDES MIRANDA HAM, VICTOR MANUEL LOYOLA VARGAS |
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Format: | info:eu-repo/semantics/article biblioteca |
Language: | eng |
Subjects: | info:eu-repo/classification/Autores/CANAVALIA ENSIFORMIS, info:eu-repo/classification/Autores/GROWTH REGULATORS, info:eu-repo/classification/Autores/NITROGEN METABOLISM, info:eu-repo/classification/cti/2, |
Online Access: | http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1254 |
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