Synthesis of oligosaccharides derived from stachyose hydrolysis by pectinex ultra SP-L
Resumen del póster presentado al XII Scientific Meeting of the Spanish Society of Chromatography and Reated Techniques (Reunión Científica de la Sociedad Española de Cromatografía y Técnicas Afines) celebrado en Tarragona del 14 al 16 de noviembre de 2012.
Saved in:
| Main Authors: | , , , |
|---|---|
| Other Authors: | |
| Format: | póster de congreso biblioteca |
| Language: | English |
| Published: |
2012
|
| Online Access: | http://hdl.handle.net/10261/63957 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100011698 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
dig-cial-es-10261-63957 |
|---|---|
| record_format |
koha |
| institution |
CIAL ES |
| collection |
DSpace |
| country |
España |
| countrycode |
ES |
| component |
Bibliográfico |
| access |
En linea |
| databasecode |
dig-cial-es |
| tag |
biblioteca |
| region |
Europa del Sur |
| libraryname |
Biblioteca del CIAL España |
| language |
English |
| description |
Resumen del póster presentado al XII Scientific Meeting of the Spanish Society of Chromatography and Reated Techniques (Reunión Científica de la Sociedad Española de Cromatografía y Técnicas Afines) celebrado en Tarragona del 14 al 16 de noviembre de 2012. |
| author2 |
Ministerio de Ciencia e Innovación (España) |
| author_facet |
Ministerio de Ciencia e Innovación (España) Montilla, Antonia Ruiz-Matute, Ana I. Olano, Agustín Corzo, Nieves |
| format |
póster de congreso |
| author |
Montilla, Antonia Ruiz-Matute, Ana I. Olano, Agustín Corzo, Nieves |
| spellingShingle |
Montilla, Antonia Ruiz-Matute, Ana I. Olano, Agustín Corzo, Nieves Synthesis of oligosaccharides derived from stachyose hydrolysis by pectinex ultra SP-L |
| author_sort |
Montilla, Antonia |
| title |
Synthesis of oligosaccharides derived from stachyose hydrolysis by pectinex ultra SP-L |
| title_short |
Synthesis of oligosaccharides derived from stachyose hydrolysis by pectinex ultra SP-L |
| title_full |
Synthesis of oligosaccharides derived from stachyose hydrolysis by pectinex ultra SP-L |
| title_fullStr |
Synthesis of oligosaccharides derived from stachyose hydrolysis by pectinex ultra SP-L |
| title_full_unstemmed |
Synthesis of oligosaccharides derived from stachyose hydrolysis by pectinex ultra SP-L |
| title_sort |
synthesis of oligosaccharides derived from stachyose hydrolysis by pectinex ultra sp-l |
| publishDate |
2012 |
| url |
http://hdl.handle.net/10261/63957 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100011698 |
| work_keys_str_mv |
AT montillaantonia synthesisofoligosaccharidesderivedfromstachyosehydrolysisbypectinexultraspl AT ruizmatuteanai synthesisofoligosaccharidesderivedfromstachyosehydrolysisbypectinexultraspl AT olanoagustin synthesisofoligosaccharidesderivedfromstachyosehydrolysisbypectinexultraspl AT corzonieves synthesisofoligosaccharidesderivedfromstachyosehydrolysisbypectinexultraspl |
| _version_ |
1777671117917388800 |
| spelling |
dig-cial-es-10261-639572020-01-13T13:32:54Z Synthesis of oligosaccharides derived from stachyose hydrolysis by pectinex ultra SP-L Montilla, Antonia Ruiz-Matute, Ana I. Olano, Agustín Corzo, Nieves Ministerio de Ciencia e Innovación (España) Comunidad de Madrid Junta de Comunidades de Castilla-La Mancha Programa Iberoamericano de Ciencia y Tecnología para el Desarrollo European Commission Resumen del póster presentado al XII Scientific Meeting of the Spanish Society of Chromatography and Reated Techniques (Reunión Científica de la Sociedad Española de Cromatografía y Técnicas Afines) celebrado en Tarragona del 14 al 16 de noviembre de 2012. a-Galactosides are derivatives of sucrose that consist of galactose residues linked by a-(1-6) linkages to the glucose moiety. These oligosaccharides are found abundantly in grain legumes and their consumption is associated with the production of flatulence. However, they might be used as a prebiotic growth substrate for intestinal bacteria because they pass undigested to the colon. Several studies provide convincing evidence that a-galactosides have beneficial effects on the survival of different bifidobacteria strains. Raffinose and stachyose are industrially available in large amounts as a by product from the production of soy protein isolate, and they seem to be a promising raw material to manufacture of new oligosaccharides. The relatively inexpensive commercial enzyme preparation Pectinex Ultra SP-L (Pectinex), produced by Aspergillus aculeatus, has been shown to contain fructosyl transferase activity and therefore, it could be used as a catalyst in the large-scale production of stachyose-derived oligosaccharides with improved prebiotic properties. The objective of this work has been to investigate in more detail the fructosyl transferase activity from A. aculeatus of the commercial enzymatic preparation Pectinex during stachyose hydrolysis. Enzymatic synthesis of oligosaccharides from stachyose using Pectinex was carried out under different reaction conditions such as temperature (50, 60, and 70 °C), pH (3.5, 4.5, 5.5, 6.5, and 7.5), stachyose concentration (100, 300, and 600 g/L), enzyme concentration (17, 34, and 78 U/ml), and time up to 24 h. Purification of the reaction mixtures was performed following the method described by Morales et al. The enriched fraction was characterized by matrixassisted laser desorption/ion1zation time of flight mass spectrometry (MALDI-TOF-MS). Mass spectra were obtained over the m/z range 100-1500. Analysis of carbohydrates was performed by gas chromatography (GC) using a flame ionization as detector (FID). The trimethylsilyl oximes of mono-, di-, and oligosaccharides were resolved using a 8 m x 0.25 mm x 0.25 J.Jm film fused silica capillary column coated with CP-SIL 5CB (methyl silicone). The oven temperature was programmed from 150 to 165 oc at 3 °C/min, then at 5 °C/min to 340ºC and held at this temperature for 10 min. Quantitative analysis was carried out by the internal standard method. The amount of remaining stachyose and the yield of oligosaccharides in the reaction mixtures were expressed as weight percentage of total carbohydrate content. Different oligosaccharides from DP2 to DP8 were formed during stachyose hydrolysis by fructosyltransferase activity of A. aculeatus. Galactosyl-melibiose (DP3) was synthesized as a result of fructosidase activity, whereas fructosyl-stachyose (DP5) and difructosyl-stachyose (DP6) were formed as a consequence of the fructosyl transferase activity of the enzyme preparation. Temperature, pH, substrate and enzyme concentrations were varied to select the optimum conditions for tri- or oligosaccharide synthesis. The optimal reaction conditions for the synthesis of penta- and hexasaccharides were 60°C, pH 5.5, 600 mg/ml of stachyose, and 34 U/ml of enzyme during 3h. However. to obtain the maximum yield of galactosyl-melibiose (67%), the assays should be carried out at 60 °C and pH 5.5, using 100 mg/ml of stachyose and 34 U/ml of enzyme during 24 h since it was a very stable to hydrolysis. In conclusion, stachyose could be used as a substrate for the enzymatic synthesis of new oligosaccharides that may open new opportunities in the development of future prebiotics. This work has been financed under a R+D program of the Spanish Ministry of Science and Innovation, Consolider Ingenio 2010 (FUN-C-FOOD): CSD 2007-00063; R + D program of the Comunidad de Madrid, project ALIBIRD 2009/AGR-1469 ;R + D program of the Comunidad de Castilla-La Mancha POII10-0178-4685 and IBEROFUN P109AC0302 (CYTED). Peer Reviewed 2013-01-10T12:00:58Z 2013-01-10T12:00:58Z 2012 2013-01-10T12:00:59Z póster de congreso http://purl.org/coar/resource_type/c_6670 SECyTA 2012 http://hdl.handle.net/10261/63957 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100011698 en #PLACEHOLDER_PARENT_METADATA_VALUE# S2009/AGR-1469/ALIBIRD Sí none |