Vascular effects of egg white-derived peptides in resistance arteries from rats. Structure-activity relationships

BACKGROUND: The vasodilator properties of several peptide sequences derived from egg white proteins were screened in mesenteric resistance arteries from Wistar-Kyoto rats. For this, third-order branches of themesenteric arteries from6-month-old male rats were used. The vasodilator responses, with or without endothelium, to several peptides (0.1mmol L-1)wereanalysed in an isometric myograph. Moreover, the effect of nitric oxide (NO) synthase (L-NAME, 100 μmol L-1) and cyclooxygenase (indomethacin, 10 μmol L-1) inhibitors on the vasodilator response was tested. RESULTS: The peptides Arg-Ala-Asp-His-Pro-Phe-Leu, Arg-Ala-Asp-His-Pro-Phe, Arg-Ala-Asp-His-Pro, Tyr-Arg-Gly-Gly-Leu-Glu-Pro-Ile-Asn-Phe, Arg-Asp-Ile-Leu-Asn-Gln and Val-Pro-Pro showed a high endothelium-dependent vasorelaxation, whereas Phe-Arg-Ala-Asp-His-Pro-Phe-Leu was only partially endothelium-dependent. The relaxation induced by Arg-Ala-Asp-His-Pro-Phe-Leu, Arg-Ala-Asp-His-Pro-Phe, Arg-Ala-Asp-His-Pro, Arg-Asp-Ile-Leu-Asn-Gln and Val-Pro-Pro wasmainlymediated by NO, since the response was inhibited only by L-NAME, while both L-NAME and indomethacin inhibited the vasodilator response induced by Phe-Arg-Ala-Asp-His-Pro-Phe-Leu and Tyr-Arg-Gly-Gly-Leu-Glu-Pro-Ile-Asn-Phe. The presence of Arg or Tyr at the N-terminal position could be related to the vasodilator activity of these compounds in this vascular bed. The well-known angiotensin-converting enzyme inhibitor captopril showed only a slight vasodilator effect. CONCLUSION: These peptides could reduce the vascular resistance and be used as functional ingredients in the prevention and/or treatment of hypertension and other associated disorders.

Bibliographic Details

Main Authors:	García-Redondo, Ana B., Roque, F. R., Miguel, Marta, López-Fandiño, Rosina, Salaices, M.
Format:	artículo biblioteca
Language:	English
Published:	Wiley-Blackwell 2010
Online Access:	http://hdl.handle.net/10261/50334
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