Macromolecular characterization of high β-glucan oat lines

Oat (Avena sativa) is a cereal grain rich in fibers, proteins, vitamins and minerals. Oats have been linked to several health benefits, such as lowering blood cholesterol levels, counteracting cardiovascular disease and regulating blood sugar levels. This study aimed to characterize two new oat lines with high β-glucan content emanating from ethyl methyl sulphonate mutagenesis on the Lantmännen elite variety Belinda. Two of the mutated lines, and the mother variety Belinda, were profiled for β-glucan, arabinoxylan, total dietary fiber and starch composition. In addition, total lipid and protein content, amino acid composition and β-glucan molecular weights were analyzed. The high levels of β-glucan resulted in a significant increase in total dietary fiber, but no correlation could be established between higher or lower levels of the assayed macromolecules, i.e., between arabinoxylan-, starch-, lipid- or protein levels in the mutated lines compared to the reference. The results indicate separate biosynthetic pathways for β-glucans and other macromolecules and an independent regulation of the different polysaccharides studied. Therefore, ethyl methyl sulphonate mutagenesis can be used to increase levels of multiple macromolecules in the same line.

Bibliographic Details

Main Authors:	Majumdar, Abhinav, Gil-González, Aina Belén, Barjuan Grau, Anna, Sardari, Roya R.R., Larsson, Olof
Other Authors:	Lund University
Format:	artículo biblioteca
Language:	English
Published:	Elsevier 2024
Subjects:	Avena sativa, Dietary fibers, β-Glucan, Macromolecular quantifications, Amino acid analysis, Molecular weight determination,
Online Access:	http://hdl.handle.net/10261/359058
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