Electrochemical bioplatform to manage alpha-gal syndrome by tracking the carbohydrate allergen in meat
This work presents the first bioplatform described to date for the determination of galactose-α-1,3-galactose (α-Gal), a non-primate mammalian oligosaccharide responsible for almost all cases of red meat allergy. The bioplatform is based on the implementation of an indirect competitive immunoassay and enzymatic labeling with the enzyme horseradish peroxidase (HRP) built on the surface of magnetic microparticles (MBs) and amperometric transduction on screen-printed carbon electrodes (SPCEs) using the H2O2/hydroquinone (HQ) system. The target α-Gal competed with biotinylated α-Gal immobilized on the surface of neutravidin-modified MBs for the limited immunorecognition sites of a detection antibody enzymatically labeled with an HRP-conjugated secondary antibody. The resulting magnetic immunoconjugates were trapped on the surface of the SPCE working electrode and amperometric transduction was performed, providing a cathodic current variation inversely proportional to the concentration of α-Gal in the analyzed sample. The developed biotool was optimized, characterized and applied with satisfactory results to the determination of the target allergen in different samples of raw and processed meats.
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Format: | artículo biblioteca |
Language: | English |
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Elsevier
2024
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Subjects: | α-Gal, Read meat allergy, Electrochemical bioplatform, Indirect competitive immunoassay, |
Online Access: | http://hdl.handle.net/10261/358859 |
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dig-cial-es-10261-3588592024-05-29T11:48:36Z Electrochemical bioplatform to manage alpha-gal syndrome by tracking the carbohydrate allergen in meat Ruiz-Valdepeñas Montiel, Víctor Gamella, María Blázquez-García, Marina Serafín, Verónica Molina, Elena Pingarrón, José Manuel Benedé, Sara Campuzano, Susana Ministerio de Ciencia, Innovación y Universidades (España) Agencia Estatal de Investigación (España) Ministerio de Ciencia e Innovación (España) Comunidad de Madrid α-Gal Read meat allergy Electrochemical bioplatform Indirect competitive immunoassay This work presents the first bioplatform described to date for the determination of galactose-α-1,3-galactose (α-Gal), a non-primate mammalian oligosaccharide responsible for almost all cases of red meat allergy. The bioplatform is based on the implementation of an indirect competitive immunoassay and enzymatic labeling with the enzyme horseradish peroxidase (HRP) built on the surface of magnetic microparticles (MBs) and amperometric transduction on screen-printed carbon electrodes (SPCEs) using the H2O2/hydroquinone (HQ) system. The target α-Gal competed with biotinylated α-Gal immobilized on the surface of neutravidin-modified MBs for the limited immunorecognition sites of a detection antibody enzymatically labeled with an HRP-conjugated secondary antibody. The resulting magnetic immunoconjugates were trapped on the surface of the SPCE working electrode and amperometric transduction was performed, providing a cathodic current variation inversely proportional to the concentration of α-Gal in the analyzed sample. The developed biotool was optimized, characterized and applied with satisfactory results to the determination of the target allergen in different samples of raw and processed meats. The financial support of Grant PID2019-103899RB-I00 funded by MCIN/AEI/10.13039/501100011033, and TRANSNANOAVANSENS-CM Program from the Comunidad de Madrid (Grant S2018/NMT-4349) to S.C. are gratefully acknowledged. Peer reviewed 2024-05-29T11:48:36Z 2024-05-29T11:48:36Z 2024 artículo Talanta 273: 125851 (2024) http://hdl.handle.net/10261/358859 10.1016/j.talanta.2024.125851 1873-3573 38447339 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-103899RB-I00/ES/BIOSENSORES PARA DESENTRAÑAR EL LEGADO Y EL FUTURO DE LA EPIGENETICA Y LA METASTASIS DEL CANCER/ S2018/NMT-4349/TRANSNANOAVANSENS-CM The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1016/j.talanta.2024.125851 https://doi.org/10.1016/j.talanta.2024.125851 Sí none Elsevier |
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α-Gal Read meat allergy Electrochemical bioplatform Indirect competitive immunoassay α-Gal Read meat allergy Electrochemical bioplatform Indirect competitive immunoassay |
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α-Gal Read meat allergy Electrochemical bioplatform Indirect competitive immunoassay α-Gal Read meat allergy Electrochemical bioplatform Indirect competitive immunoassay Ruiz-Valdepeñas Montiel, Víctor Gamella, María Blázquez-García, Marina Serafín, Verónica Molina, Elena Pingarrón, José Manuel Benedé, Sara Campuzano, Susana Electrochemical bioplatform to manage alpha-gal syndrome by tracking the carbohydrate allergen in meat |
description |
This work presents the first bioplatform described to date for the determination of galactose-α-1,3-galactose (α-Gal), a non-primate mammalian oligosaccharide responsible for almost all cases of red meat allergy. The bioplatform is based on the implementation of an indirect competitive immunoassay and enzymatic labeling with the enzyme horseradish peroxidase (HRP) built on the surface of magnetic microparticles (MBs) and amperometric transduction on screen-printed carbon electrodes (SPCEs) using the H2O2/hydroquinone (HQ) system. The target α-Gal competed with biotinylated α-Gal immobilized on the surface of neutravidin-modified MBs for the limited immunorecognition sites of a detection antibody enzymatically labeled with an HRP-conjugated secondary antibody. The resulting magnetic immunoconjugates were trapped on the surface of the SPCE working electrode and amperometric transduction was performed, providing a cathodic current variation inversely proportional to the concentration of α-Gal in the analyzed sample. The developed biotool was optimized, characterized and applied with satisfactory results to the determination of the target allergen in different samples of raw and processed meats. |
author2 |
Ministerio de Ciencia, Innovación y Universidades (España) |
author_facet |
Ministerio de Ciencia, Innovación y Universidades (España) Ruiz-Valdepeñas Montiel, Víctor Gamella, María Blázquez-García, Marina Serafín, Verónica Molina, Elena Pingarrón, José Manuel Benedé, Sara Campuzano, Susana |
format |
artículo |
topic_facet |
α-Gal Read meat allergy Electrochemical bioplatform Indirect competitive immunoassay |
author |
Ruiz-Valdepeñas Montiel, Víctor Gamella, María Blázquez-García, Marina Serafín, Verónica Molina, Elena Pingarrón, José Manuel Benedé, Sara Campuzano, Susana |
author_sort |
Ruiz-Valdepeñas Montiel, Víctor |
title |
Electrochemical bioplatform to manage alpha-gal syndrome by tracking the carbohydrate allergen in meat |
title_short |
Electrochemical bioplatform to manage alpha-gal syndrome by tracking the carbohydrate allergen in meat |
title_full |
Electrochemical bioplatform to manage alpha-gal syndrome by tracking the carbohydrate allergen in meat |
title_fullStr |
Electrochemical bioplatform to manage alpha-gal syndrome by tracking the carbohydrate allergen in meat |
title_full_unstemmed |
Electrochemical bioplatform to manage alpha-gal syndrome by tracking the carbohydrate allergen in meat |
title_sort |
electrochemical bioplatform to manage alpha-gal syndrome by tracking the carbohydrate allergen in meat |
publisher |
Elsevier |
publishDate |
2024 |
url |
http://hdl.handle.net/10261/358859 |
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