Vasoactive properties of a cocoa shell extract: Mechanism of action and effect on endothelial dysfunction in aged rats
This article belongs to the Special Issue Antioxidant Foods and Cardiometabolic Health.
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Multidisciplinary Digital Publishing Institute
2022
|
| Subjects: | Cocoa shell, Caffeine, Endothelial dysfunction, By-products revalorization, Nitric oxide, Protocatechuic acid, Theobromine, Superoxide anion, |
| Online Access: | http://hdl.handle.net/10261/337228 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
dig-cial-es-10261-337228 |
|---|---|
| record_format |
koha |
| spelling |
dig-cial-es-10261-3372282023-10-17T07:35:39Z Vasoactive properties of a cocoa shell extract: Mechanism of action and effect on endothelial dysfunction in aged rats Rodríguez-Rodríguez, Pilar Ragusky, Kendal Phuthong, Sophida Ruvira, Santiago Ramiro-Cortijo, David Cañas, Silvia Rebollo-Hernanz, Miguel Morales, María Dolores López de Pablo, Ángel L. Martín-Cabrejas, María A. Arribas, Silvia M. Ministerio de Ciencia, Innovación y Universidades (España) Agencia Estatal de Investigación (España) Ministerio de Universidades (España) Comunidad de Madrid Universidad Autónoma de Madrid Cocoa shell Caffeine Endothelial dysfunction By-products revalorization Nitric oxide Protocatechuic acid Theobromine Superoxide anion This article belongs to the Special Issue Antioxidant Foods and Cardiometabolic Health. The data presented in this study are available on request from the corresponding author. The availability of the data is restricted to investigators-based in academic institutions. Cocoa has cardiovascular beneficial effects related to its content of antioxidant phytochemicals. Cocoa manufacturing produces large amounts of waste, but some by-products may be used as ingredients with health-promoting potential. We aimed to investigate the vasoactive actions of an extract from cocoa shell (CSE), a by-product containing theobromine (TH), caffeine (CAF) and protocatechuic acid (PCA) as major phytochemicals. In carotid and iliac arteries from 5-month and 15-month-old rats, we investigated CSE vasoactive properties, mechanism of action, and the capacity of CSE, TH, CAF and PCA to improve age-induced endothelial dysfunction. Vascular function was evaluated using isometric tension recording and superoxide anion production by dihydroethidium (DHE) staining and confocal microscopy. CSE caused endothelium-dependent vasorelaxation, blocked by L-NAME, but not indomethacin, regardless of sex, age, or vessel type. CSE maximal responses and EC50 were significantly lower compared to acetylcholine (ACh). Arterial preincubation with CSE, TH, CAF or PCA, significantly reduced the number of vascular DHE-positive cells. Compared to adult males, iliac arteries from aged males exhibited reduced ACh concentration-dependent vasodilatation but larger CSE responses. In iliac arteries from aged male and female rats, preincubation with 10−4 M CSE and PCA, but not TH or CAF, improved ACh-relaxations. In conclusion, CSE has vasodilatory properties associated with increased nitric oxide bioavailability, related to its antioxidant phytochemicals, being particularly relevant PCA. Therefore, CSE is a potential food ingredient for diseases related to endothelial dysfunction. This research was funded by the COCARDIOLAC project from the Spanish Ministry of Science and Innovation (RTI 2018-097504-B-I00). Rebollo-Hernanz received funding from the FPU program of the Spanish Ministry of Universities for his predoctoral fellowship (FPU15/04238) and Margarita Salas Contract (CA1/RSUE/2021-00656). Martín-Cabrejas thanks to the Excellence Line for University Teaching Staff within the Multiannual Agreement between the Community of Madrid and the UAM (2019–2023). Peer reviewed 2023-10-17T07:34:32Z 2023-10-17T07:34:32Z 2022 artículo Antioxidants 11(2): 429 (2022) http://hdl.handle.net/10261/337228 10.3390/antiox11020429 2076-3921 35204310 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-097504-B-I00/ES/NUEVOS INGREDIENTES ANTIOXIDANTES DE SUBPRODUCTOS DE CAFE Y CACAO COMO ESTRATEGIA PARA REPROGRAMAR LA ENFERMEDAD CARDIOMETABOLICA A TRAVES DE LA LACTANCIA/ Publisher's version The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.3390/antiox11020429 https://doi.org/10.3390/antiox11020429 Sí open application/pdf Multidisciplinary Digital Publishing Institute |
| institution |
CIAL ES |
| collection |
DSpace |
| country |
España |
| countrycode |
ES |
| component |
Bibliográfico |
| access |
En linea |
| databasecode |
dig-cial-es |
| tag |
biblioteca |
| region |
Europa del Sur |
| libraryname |
Biblioteca del CIAL España |
| language |
English |
| topic |
Cocoa shell Caffeine Endothelial dysfunction By-products revalorization Nitric oxide Protocatechuic acid Theobromine Superoxide anion Cocoa shell Caffeine Endothelial dysfunction By-products revalorization Nitric oxide Protocatechuic acid Theobromine Superoxide anion |
| spellingShingle |
Cocoa shell Caffeine Endothelial dysfunction By-products revalorization Nitric oxide Protocatechuic acid Theobromine Superoxide anion Cocoa shell Caffeine Endothelial dysfunction By-products revalorization Nitric oxide Protocatechuic acid Theobromine Superoxide anion Rodríguez-Rodríguez, Pilar Ragusky, Kendal Phuthong, Sophida Ruvira, Santiago Ramiro-Cortijo, David Cañas, Silvia Rebollo-Hernanz, Miguel Morales, María Dolores López de Pablo, Ángel L. Martín-Cabrejas, María A. Arribas, Silvia M. Vasoactive properties of a cocoa shell extract: Mechanism of action and effect on endothelial dysfunction in aged rats |
| description |
This article belongs to the Special Issue Antioxidant Foods and Cardiometabolic Health. |
| author2 |
Ministerio de Ciencia, Innovación y Universidades (España) |
| author_facet |
Ministerio de Ciencia, Innovación y Universidades (España) Rodríguez-Rodríguez, Pilar Ragusky, Kendal Phuthong, Sophida Ruvira, Santiago Ramiro-Cortijo, David Cañas, Silvia Rebollo-Hernanz, Miguel Morales, María Dolores López de Pablo, Ángel L. Martín-Cabrejas, María A. Arribas, Silvia M. |
| format |
artículo |
| topic_facet |
Cocoa shell Caffeine Endothelial dysfunction By-products revalorization Nitric oxide Protocatechuic acid Theobromine Superoxide anion |
| author |
Rodríguez-Rodríguez, Pilar Ragusky, Kendal Phuthong, Sophida Ruvira, Santiago Ramiro-Cortijo, David Cañas, Silvia Rebollo-Hernanz, Miguel Morales, María Dolores López de Pablo, Ángel L. Martín-Cabrejas, María A. Arribas, Silvia M. |
| author_sort |
Rodríguez-Rodríguez, Pilar |
| title |
Vasoactive properties of a cocoa shell extract: Mechanism of action and effect on endothelial dysfunction in aged rats |
| title_short |
Vasoactive properties of a cocoa shell extract: Mechanism of action and effect on endothelial dysfunction in aged rats |
| title_full |
Vasoactive properties of a cocoa shell extract: Mechanism of action and effect on endothelial dysfunction in aged rats |
| title_fullStr |
Vasoactive properties of a cocoa shell extract: Mechanism of action and effect on endothelial dysfunction in aged rats |
| title_full_unstemmed |
Vasoactive properties of a cocoa shell extract: Mechanism of action and effect on endothelial dysfunction in aged rats |
| title_sort |
vasoactive properties of a cocoa shell extract: mechanism of action and effect on endothelial dysfunction in aged rats |
| publisher |
Multidisciplinary Digital Publishing Institute |
| publishDate |
2022 |
| url |
http://hdl.handle.net/10261/337228 |
| work_keys_str_mv |
AT rodriguezrodriguezpilar vasoactivepropertiesofacocoashellextractmechanismofactionandeffectonendothelialdysfunctioninagedrats AT raguskykendal vasoactivepropertiesofacocoashellextractmechanismofactionandeffectonendothelialdysfunctioninagedrats AT phuthongsophida vasoactivepropertiesofacocoashellextractmechanismofactionandeffectonendothelialdysfunctioninagedrats AT ruvirasantiago vasoactivepropertiesofacocoashellextractmechanismofactionandeffectonendothelialdysfunctioninagedrats AT ramirocortijodavid vasoactivepropertiesofacocoashellextractmechanismofactionandeffectonendothelialdysfunctioninagedrats AT canassilvia vasoactivepropertiesofacocoashellextractmechanismofactionandeffectonendothelialdysfunctioninagedrats AT rebollohernanzmiguel vasoactivepropertiesofacocoashellextractmechanismofactionandeffectonendothelialdysfunctioninagedrats AT moralesmariadolores vasoactivepropertiesofacocoashellextractmechanismofactionandeffectonendothelialdysfunctioninagedrats AT lopezdepabloangell vasoactivepropertiesofacocoashellextractmechanismofactionandeffectonendothelialdysfunctioninagedrats AT martincabrejasmariaa vasoactivepropertiesofacocoashellextractmechanismofactionandeffectonendothelialdysfunctioninagedrats AT arribassilviam vasoactivepropertiesofacocoashellextractmechanismofactionandeffectonendothelialdysfunctioninagedrats |
| _version_ |
1781881434477166592 |