Peptidomic characterization and amino acid availability after intake of casein vs. a casein hydrolysate in a pig model

Peptidomic characterization and amino acid availability after intake of casein vs. a casein hydrolysate in a pig model

This article belongs to the Special Issue Role of Food Digestion and Digestive System in the Nutritional, Functional and Health Properties of Food Bioactives.

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Bibliographic Details
Main Authors: Jiménez-Barrios, Pablo, Sánchez Rivera, Laura, Martínez-Maqueda, D., Le Gouar, Yann, Dupont, Didier, Miralles, Beatriz, Recio, Isidra
Other Authors: Ministerio de Ciencia, Innovación y Universidades (España)
Format: artículo biblioteca
Language:English
Published: Multidisciplinary Digital Publishing Institute 2023
Subjects:Casein, Digestion kinetics, Hydrolysate, Peptidomics, Duodenal digest,
Online Access:http://hdl.handle.net/10261/311531
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spelling dig-cial-es-10261-3115312023-06-15T07:12:09Z Peptidomic characterization and amino acid availability after intake of casein vs. a casein hydrolysate in a pig model Jiménez-Barrios, Pablo Sánchez Rivera, Laura Martínez-Maqueda, D. Le Gouar, Yann Dupont, Didier Miralles, Beatriz Recio, Isidra Ministerio de Ciencia, Innovación y Universidades (España) Ministerio de Ciencia e Innovación (España) Agencia Estatal de Investigación (España) Casein Digestion kinetics Hydrolysate Peptidomics Duodenal digest This article belongs to the Special Issue Role of Food Digestion and Digestive System in the Nutritional, Functional and Health Properties of Food Bioactives. It is known that casein hydrolysis accelerates gastrointestinal transit in comparison to intact casein, although the effect of the protein hydrolysis on the composition of the digests is not fully understood. The aim of this work is to characterize, at the peptidome level, duodenal digests from pigs, as a model of human digestion, fed with micellar casein and a previously described casein hydrolysate. In addition, in parallel experiments, plasma amino acid levels were quantified. A slower transit of nitrogen to the duodenum was found when the animals received micellar casein. Duodenal digests from casein contained a wider range of peptide sizes and a higher number of peptides above five amino acids long in comparison with the digests from the hydrolysate. The peptide profile was markedly different, and although β-casomorphin-7 precursors were also found in hydrolysate samples, other opioid sequences were more abundant in the casein digests. Within the same substrate, the evolution of the peptide pattern at different time points showed minimal changes, suggesting that the protein degradation rate relies more on the gastrointestinal location than on digestion time. Higher plasma concentrations of methionine, valine, lysine and amino acid metabolites were found in animals fed with the hydrolysate at short times (<200 min). The duodenal peptide profiles were evaluated with discriminant analysis tools specific for peptidomics to identify sequence differences between both substrates that can be used for future human physiological and metabolic studies. This work has received financial support from grants PID2019-107663RB-I00 and PDC2022-133489-I00 funded by MCIN/AEI/10.13039/501100011033. Peer reviewed 2023-06-15T07:12:09Z 2023-06-15T07:12:09Z 2023 artículo Nutrients 15(5): 1065 (2023) http://hdl.handle.net/10261/311531 10.3390/nu15051065 2072-6643 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-107663RB-I00/ES/EVALUACION DE LA CALIDAD PROTEICA. SEÑALIZACION INTESTINAL DE LOS PRODUCTOS DE DIGESTION DE LAS PROTEINAS ALIMENTARIAS EN LA INDUCCION DE LA SACIEDAD/ info:eu-repo/grantAgreement/AEI//PDC2022-133489-I00 Nutrients Publisher's version https://doi.org/10.3390/nu15051065 Sí open application/pdf Multidisciplinary Digital Publishing Institute
institution CIAL ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-cial-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del CIAL España
language English
topic Casein
Digestion kinetics
Hydrolysate
Peptidomics
Duodenal digest
Casein
Digestion kinetics
Hydrolysate
Peptidomics
Duodenal digest
spellingShingle Casein
Digestion kinetics
Hydrolysate
Peptidomics
Duodenal digest
Casein
Digestion kinetics
Hydrolysate
Peptidomics
Duodenal digest
Jiménez-Barrios, Pablo
Sánchez Rivera, Laura
Martínez-Maqueda, D.
Le Gouar, Yann
Dupont, Didier
Miralles, Beatriz
Recio, Isidra
Peptidomic characterization and amino acid availability after intake of casein vs. a casein hydrolysate in a pig model
description This article belongs to the Special Issue Role of Food Digestion and Digestive System in the Nutritional, Functional and Health Properties of Food Bioactives.
author2 Ministerio de Ciencia, Innovación y Universidades (España)
author_facet Ministerio de Ciencia, Innovación y Universidades (España)
Jiménez-Barrios, Pablo
Sánchez Rivera, Laura
Martínez-Maqueda, D.
Le Gouar, Yann
Dupont, Didier
Miralles, Beatriz
Recio, Isidra
format artículo
topic_facet Casein
Digestion kinetics
Hydrolysate
Peptidomics
Duodenal digest
author Jiménez-Barrios, Pablo
Sánchez Rivera, Laura
Martínez-Maqueda, D.
Le Gouar, Yann
Dupont, Didier
Miralles, Beatriz
Recio, Isidra
author_sort Jiménez-Barrios, Pablo
title Peptidomic characterization and amino acid availability after intake of casein vs. a casein hydrolysate in a pig model
title_short Peptidomic characterization and amino acid availability after intake of casein vs. a casein hydrolysate in a pig model
title_full Peptidomic characterization and amino acid availability after intake of casein vs. a casein hydrolysate in a pig model
title_fullStr Peptidomic characterization and amino acid availability after intake of casein vs. a casein hydrolysate in a pig model
title_full_unstemmed Peptidomic characterization and amino acid availability after intake of casein vs. a casein hydrolysate in a pig model
title_sort peptidomic characterization and amino acid availability after intake of casein vs. a casein hydrolysate in a pig model
publisher Multidisciplinary Digital Publishing Institute
publishDate 2023
url http://hdl.handle.net/10261/311531
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