Supplemental material for The role of spray-drying atmosphere on fridericia chica (bonpl.) L.G. Lohmann standardized extract production for wound healing activity

Fridericia chica (Bonpl.) L.G. Lohmann (synonym Arrabidaea chica Verlot) (Bignoniaceae) is widely used in folk medicine in Brazil for health benefits. Considering overcoming pitfalls of scaling up production of plant extracts, herein the effects of N2 atomizing gas in spray-drying process of standardized plant extract production is reported. Samples were monitored by in vitro antioxidant activity and microbiological evaluation. The type of gas used did not interfere in the product’s color. However, the drying atmosphere influenced 3-deoxyanthocyanines content when using air as atomizing gas, decreasing carajurin (37.5%) content with concomitant increase in luteolin yield (24.1%). Both drying processes preserved the samples’ pharmacological profile. The antioxidant activity was also similar IC50 (445.60 ± 12.73 and 466.99 ± 46.11). In the cell migration test with HaCaT cells, the extract dried under air flow (5 μg/mL) promoted wound closure by 78% (12 hours) whereas the extract dried using N2 flow promoted 49% (12 hours), with 98% closure (12 hours) for the positive control. The antimicrobial evaluation for Staphylococcus aureus did not differ with the different drying atmospheres, with MIC (minimum inhibitory concentration) at 0.39 mg/mL. Therefore, the drying process reported herein did not interfere with the outcome of the biological activity.

Bibliographic Details

Main Authors:	Oliveira Sousa, Ilza Maria de, Álvarez-Rivera, Gerardo, Pinto, Diana, Cássia Almeida Queiroz, Nubia de, Correia Bastos, Veronica Isabel, Braga, Lucia Elaine O., Dantas Palmeira, Josman, Amaral, Helena, Neto Ferreira, Helena Maria, Correia Oliveira, Helena Cristina, Mendes Gonçalves, Fernando J., Ruiz, Ana Lucia, Ibáñez, Elena, Oliveira, Beatriz P.P., Foglio, Mary A.
Format:	dataset biblioteca
Language:	English
Published:	Taylor & Francis 2022
Subjects:	Fridericia chica, Arrabidaea chica, Spray drying, Toxicity, Antioxidant, Wound healing,
Online Access:	http://hdl.handle.net/10261/311319
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