Production and evaluation of two antibiotics of Streptomyces coelicolor A3(2), prodigiosin and actinorhodin under solid state fermentation, using micro-porous culture
In this study solid state fermentation (SSF), was evaluated using a novel PolyHIPE Polymer (PHP) matrix. The matrix was developed with approach for improving the production of antibiotic from Streptomyces coelicolor A3(2), made-up with different pore sizes (55, 39, 19 µm) with surface chemistry Sulphonated/Neutralised. The matrix was packed in micro-bioreactor for the production of antibiotics, the growth conditions such as pores size of the matrix was investigated against the growth time (From 0 to 168 hours) and two the extracts were produced, Prodigiosin a member of tripyrrole family red pigment and Actinorhodin a dimeric benzoisochromanequinone antibiotic which belongs to a class of aromatic polyketides. The concentration of extracted antibiotics and their activities were examined by disk diffusion method. The two produced compounds were tested against the microbial strains (Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas fluorescens, Penicillium notatum) and the inhibition effects were measured. As a result, it was concluded that the largest zone of inhibition (diameter in mm) was observed in the plate inoculated with the strains of DSM 10 (Bacillus subtilis), inhibited by prodigiosin extract.
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Elsevier
2022
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Online Access: | http://hdl.handle.net/10261/279280 http://dx.doi.org/10.13039/501100005983 http://dx.doi.org/10.13039/501100000266 http://dx.doi.org/10.13039/501100000781 |
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dig-cial-es-10261-2792802022-11-04T17:38:05Z Production and evaluation of two antibiotics of Streptomyces coelicolor A3(2), prodigiosin and actinorhodin under solid state fermentation, using micro-porous culture Victor, Teresa Matoso M. Ndlovu, Teresa M. Filho, Miguel Pessela, Benevides C. Bull, Steve Ward, Alan C. Engineering and Physical Sciences Research Council (UK) European Research Council Department of Trade and Industry (UK) In this study solid state fermentation (SSF), was evaluated using a novel PolyHIPE Polymer (PHP) matrix. The matrix was developed with approach for improving the production of antibiotic from Streptomyces coelicolor A3(2), made-up with different pore sizes (55, 39, 19 µm) with surface chemistry Sulphonated/Neutralised. The matrix was packed in micro-bioreactor for the production of antibiotics, the growth conditions such as pores size of the matrix was investigated against the growth time (From 0 to 168 hours) and two the extracts were produced, Prodigiosin a member of tripyrrole family red pigment and Actinorhodin a dimeric benzoisochromanequinone antibiotic which belongs to a class of aromatic polyketides. The concentration of extracted antibiotics and their activities were examined by disk diffusion method. The two produced compounds were tested against the microbial strains (Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas fluorescens, Penicillium notatum) and the inhibition effects were measured. As a result, it was concluded that the largest zone of inhibition (diameter in mm) was observed in the plate inoculated with the strains of DSM 10 (Bacillus subtilis), inhibited by prodigiosin extract. We acknowledge the support received from the Engineering and Physical Sciences Research Council (UK), European Research Council, Department of Trade and Industry (DTI) (UK), and we thank the members of staff at the SEM Chemical unit and medical unit for the technical support. Peer reviewed 2022-09-16T09:31:23Z 2022-09-16T09:31:23Z 2022 artículo Chemical Engineering and Processing - Process Intensification 170: 108685 (2022) 0255-2701 http://hdl.handle.net/10261/279280 10.1016/j.cep.2021.108685 http://dx.doi.org/10.13039/501100005983 http://dx.doi.org/10.13039/501100000266 http://dx.doi.org/10.13039/501100000781 en https://doi.org/10.1016/j.cep.2021.108685 Sí none Elsevier |
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In this study solid state fermentation (SSF), was evaluated using a novel PolyHIPE Polymer (PHP) matrix. The matrix was developed with approach for improving the production of antibiotic from Streptomyces coelicolor A3(2), made-up with different pore sizes (55, 39, 19 µm) with surface chemistry Sulphonated/Neutralised. The matrix was packed in micro-bioreactor for the production of antibiotics, the growth conditions such as pores size of the matrix was investigated against the growth time (From 0 to 168 hours) and two the extracts were produced, Prodigiosin a member of tripyrrole family red pigment and Actinorhodin a dimeric benzoisochromanequinone antibiotic which belongs to a class of aromatic polyketides. The concentration of extracted antibiotics and their activities were examined by disk diffusion method. The two produced compounds were tested against the microbial strains (Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas fluorescens, Penicillium notatum) and the inhibition effects were measured. As a result, it was concluded that the largest zone of inhibition (diameter in mm) was observed in the plate inoculated with the strains of DSM 10 (Bacillus subtilis), inhibited by prodigiosin extract. |
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Engineering and Physical Sciences Research Council (UK) |
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Engineering and Physical Sciences Research Council (UK) Victor, Teresa Matoso M. Ndlovu, Teresa M. Filho, Miguel Pessela, Benevides C. Bull, Steve Ward, Alan C. |
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artículo |
author |
Victor, Teresa Matoso M. Ndlovu, Teresa M. Filho, Miguel Pessela, Benevides C. Bull, Steve Ward, Alan C. |
spellingShingle |
Victor, Teresa Matoso M. Ndlovu, Teresa M. Filho, Miguel Pessela, Benevides C. Bull, Steve Ward, Alan C. Production and evaluation of two antibiotics of Streptomyces coelicolor A3(2), prodigiosin and actinorhodin under solid state fermentation, using micro-porous culture |
author_sort |
Victor, Teresa Matoso M. |
title |
Production and evaluation of two antibiotics of Streptomyces coelicolor A3(2), prodigiosin and actinorhodin under solid state fermentation, using micro-porous culture |
title_short |
Production and evaluation of two antibiotics of Streptomyces coelicolor A3(2), prodigiosin and actinorhodin under solid state fermentation, using micro-porous culture |
title_full |
Production and evaluation of two antibiotics of Streptomyces coelicolor A3(2), prodigiosin and actinorhodin under solid state fermentation, using micro-porous culture |
title_fullStr |
Production and evaluation of two antibiotics of Streptomyces coelicolor A3(2), prodigiosin and actinorhodin under solid state fermentation, using micro-porous culture |
title_full_unstemmed |
Production and evaluation of two antibiotics of Streptomyces coelicolor A3(2), prodigiosin and actinorhodin under solid state fermentation, using micro-porous culture |
title_sort |
production and evaluation of two antibiotics of streptomyces coelicolor a3(2), prodigiosin and actinorhodin under solid state fermentation, using micro-porous culture |
publisher |
Elsevier |
publishDate |
2022 |
url |
http://hdl.handle.net/10261/279280 http://dx.doi.org/10.13039/501100005983 http://dx.doi.org/10.13039/501100000266 http://dx.doi.org/10.13039/501100000781 |
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