Ultrasound-assisted “green” extraction (UAE) of antioxidant compounds (betalains and phenolics) from Opuntia stricta var. Dilenii’s fruits: optimization and biological activities
This article belongs to the Special Issue Secondary Metabolites Extraction and/or Functionalization: from Industrial Co-product to Cosmetic Applications.
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Multidisciplinary Digital Publishing Institute
2021
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Subjects: | Opuntia stricta var. Dillenii whole fruits, Anti-inflammatory activity, Ultrasound-assisted extraction, Antioxidant activity, Green solvents, Phenolic compounds, |
Online Access: | http://hdl.handle.net/10261/263473 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100004837 |
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dig-cial-es-10261-2634732022-03-10T03:30:14Z Ultrasound-assisted “green” extraction (UAE) of antioxidant compounds (betalains and phenolics) from Opuntia stricta var. Dilenii’s fruits: optimization and biological activities Gómez-López, Iván Lobo-Rodrigo, Gloria Portillo, María P. Cano, M. Pilar Ministerio de Ciencia e Innovación (España) CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) Ministerio de Ciencia, Innovación y Universidades (España) Agencia Estatal de Investigación (España) Opuntia stricta var. Dillenii whole fruits Anti-inflammatory activity Ultrasound-assisted extraction Antioxidant activity Green solvents Phenolic compounds This article belongs to the Special Issue Secondary Metabolites Extraction and/or Functionalization: from Industrial Co-product to Cosmetic Applications. Opuntia stricta var. Dillenii’s prickly pears are an underutilized fruit with a high content of betalains and phenolic compounds that could bring potential health benefits for humans. The aim of this study is the optimization of the “green” extraction of betalains and phenolic compounds from Opuntia stricta var. Dillenii’s whole fruits by ultrasound-assisted extraction (UAE), using a response surface methodology (RSM) by a central composite design (CCD) in order to obtain extracts rich in betalains and phenolic compounds with proven biological activities. For UAE optimization, the extraction temperature (20–50 °C), the amplitude (20–50%) and the ethanol volume in extraction solvent (15–80%, v/v) were selected as independent variables. All combinations were conducted at 2, 5, 10, 20 and 30 min to determinate the time effect. The betalain and phenolic compound content in Opuntia stricta var. Dillenii’s whole fruits and UAE extracts were identified by HPLC-DAD-ESI/MS and HPLC-DAD-MS/QTOF and the antioxidant (ORAC method) and the anti-inflammatory (hyaluronidase inhibition method) in vitro biological activities also were determined. The most efficient extraction time was 5 min and the best UAE parameter combination was 50% amplitude, 15% ethanol in solvent (ethanol/water, 15/85, v/v) and 20 °C temperature, obtaining 10.06 ± 0.10 mg of total major betalains/g dry weight, 2.32 ± 0.08 mg of piscidic acid/g dry weight and 0.38 ± 0.00 mg of total major flavonoids/g dry weight. All applied UAE combinations significantly improved the in vitro bioactive activities (antioxidant and anti-inflammatory) of the Opuntia stricta var. Dillenii’s extracts compared to the bioactivities of the extracts obtained by standard homogenization processes. This research was funded by the Spanish Ministry of Science and Innovation, project number RTA2015-00044-C02-02 and number PID2020-118300RB-C21. Peer reviewed 2022-03-09T11:30:03Z 2022-03-09T11:30:03Z 2021 artículo http://purl.org/coar/resource_type/c_6501 Antioxidants 10(11): 1786 (2021) http://hdl.handle.net/10261/263473 10.3390/antiox10111786 2076-3921 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100004837 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-118300RB-C21/ES/PROCESOS INNOVADORES GREEN PARA EL DESARROLLO DE NUEVAS FORMULACIONES INTELIGENTES A PARTIR DE LOS SUBPRODUCTOS DE FRUTOS DE OPUNTIA SPP., PARA LA PREVENCION DE LA ESTEANOSI/ Publisher's version https://doi.org/10.3390/antiox10111786 Sí open Multidisciplinary Digital Publishing Institute |
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Opuntia stricta var. Dillenii whole fruits Anti-inflammatory activity Ultrasound-assisted extraction Antioxidant activity Green solvents Phenolic compounds Opuntia stricta var. Dillenii whole fruits Anti-inflammatory activity Ultrasound-assisted extraction Antioxidant activity Green solvents Phenolic compounds |
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Opuntia stricta var. Dillenii whole fruits Anti-inflammatory activity Ultrasound-assisted extraction Antioxidant activity Green solvents Phenolic compounds Opuntia stricta var. Dillenii whole fruits Anti-inflammatory activity Ultrasound-assisted extraction Antioxidant activity Green solvents Phenolic compounds Gómez-López, Iván Lobo-Rodrigo, Gloria Portillo, María P. Cano, M. Pilar Ultrasound-assisted “green” extraction (UAE) of antioxidant compounds (betalains and phenolics) from Opuntia stricta var. Dilenii’s fruits: optimization and biological activities |
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This article belongs to the Special Issue Secondary Metabolites Extraction and/or Functionalization: from Industrial Co-product to Cosmetic Applications. |
author2 |
Ministerio de Ciencia e Innovación (España) |
author_facet |
Ministerio de Ciencia e Innovación (España) Gómez-López, Iván Lobo-Rodrigo, Gloria Portillo, María P. Cano, M. Pilar |
format |
artículo |
topic_facet |
Opuntia stricta var. Dillenii whole fruits Anti-inflammatory activity Ultrasound-assisted extraction Antioxidant activity Green solvents Phenolic compounds |
author |
Gómez-López, Iván Lobo-Rodrigo, Gloria Portillo, María P. Cano, M. Pilar |
author_sort |
Gómez-López, Iván |
title |
Ultrasound-assisted “green” extraction (UAE) of antioxidant compounds (betalains and phenolics) from Opuntia stricta var. Dilenii’s fruits: optimization and biological activities |
title_short |
Ultrasound-assisted “green” extraction (UAE) of antioxidant compounds (betalains and phenolics) from Opuntia stricta var. Dilenii’s fruits: optimization and biological activities |
title_full |
Ultrasound-assisted “green” extraction (UAE) of antioxidant compounds (betalains and phenolics) from Opuntia stricta var. Dilenii’s fruits: optimization and biological activities |
title_fullStr |
Ultrasound-assisted “green” extraction (UAE) of antioxidant compounds (betalains and phenolics) from Opuntia stricta var. Dilenii’s fruits: optimization and biological activities |
title_full_unstemmed |
Ultrasound-assisted “green” extraction (UAE) of antioxidant compounds (betalains and phenolics) from Opuntia stricta var. Dilenii’s fruits: optimization and biological activities |
title_sort |
ultrasound-assisted “green” extraction (uae) of antioxidant compounds (betalains and phenolics) from opuntia stricta var. dilenii’s fruits: optimization and biological activities |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021 |
url |
http://hdl.handle.net/10261/263473 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100004837 |
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