Short-term effect of egg-white hydrolysate products on the arterial blood pressure of hypertensive rats
In the present study we evaluate the blood pressure-lowering effect of the following products: the hydrolysate obtained from egg white (EW) by enzymatic treatment with pepsin (HEW), the peptide fraction of HEW with molecular mass lower than 3000 Da (HEW<3000 Da), and three peptide sequences isolated from HEW<3000 Da (Tyr-Ala-Glu-Glu-Arg-Tyr-Pro-Ile-Leu: YAEERYPIL); (Arg-Ala-Asp-His-Pro-Phe-Leu: RADHPFL); and (Ile-Val-Phe (IVF)). These peptides, and also HEW and HEW<3000 Da, had been characterized previously in vitro as potent inhibitors of angiotensin-converting enzyme (ACE). EW and the products mentioned earlier were orally administered by gastric intubation, to 17–20-week-old male spontaneously hypertensive rats (SHR) and normotensive Wistar–Kyoto (WKY) rats. We measured the systolic blood pressure (SBP) and the diastolic blood pressure (DBP) of the rats by the tail cuff method before administration and also 2, 4, 6, 8 and 24h post-administration. Distilled water served as negative control, and we used captopril (50mg/kg) as positive control to carry out similar experiments with a known ACE inhibitor. HEW, HEW<3000 Da and the three peptide sequences decreased SBP and DBP in SHR but they did not modify these variables in WKY rats. The peptide sequences YAEERYPIL, RADHPFL and IVF showed a potency to decrease blood pressure greater than HEW or HEW<3000 Da. The results obtained suggest that the studied products could be used as a functional food with potential therapeutic benefit in the prevention and treatment of hypertension.
Main Authors: | , , , |
---|---|
Other Authors: | |
Format: | artículo biblioteca |
Published: |
Cambridge University Press
2005-11
|
Subjects: | Hypertension, Angiotensin-converting enzyme, Bioactive peptides, Spontaneously hypertensive rats, |
Online Access: | http://hdl.handle.net/10261/256447 http://dx.doi.org/10.13039/501100006280 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cial-es-10261-256447 |
---|---|
record_format |
koha |
spelling |
dig-cial-es-10261-2564472022-01-31T16:59:22Z Short-term effect of egg-white hydrolysate products on the arterial blood pressure of hypertensive rats Miguel, Marta López-Fandiño, Rosina Ramos, Mercedes Aleixandre, Amaya CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) Ministerio de Ciencia y Tecnología (España) Hypertension Angiotensin-converting enzyme Bioactive peptides Spontaneously hypertensive rats In the present study we evaluate the blood pressure-lowering effect of the following products: the hydrolysate obtained from egg white (EW) by enzymatic treatment with pepsin (HEW), the peptide fraction of HEW with molecular mass lower than 3000 Da (HEW<3000 Da), and three peptide sequences isolated from HEW<3000 Da (Tyr-Ala-Glu-Glu-Arg-Tyr-Pro-Ile-Leu: YAEERYPIL); (Arg-Ala-Asp-His-Pro-Phe-Leu: RADHPFL); and (Ile-Val-Phe (IVF)). These peptides, and also HEW and HEW<3000 Da, had been characterized previously in vitro as potent inhibitors of angiotensin-converting enzyme (ACE). EW and the products mentioned earlier were orally administered by gastric intubation, to 17–20-week-old male spontaneously hypertensive rats (SHR) and normotensive Wistar–Kyoto (WKY) rats. We measured the systolic blood pressure (SBP) and the diastolic blood pressure (DBP) of the rats by the tail cuff method before administration and also 2, 4, 6, 8 and 24h post-administration. Distilled water served as negative control, and we used captopril (50mg/kg) as positive control to carry out similar experiments with a known ACE inhibitor. HEW, HEW<3000 Da and the three peptide sequences decreased SBP and DBP in SHR but they did not modify these variables in WKY rats. The peptide sequences YAEERYPIL, RADHPFL and IVF showed a potency to decrease blood pressure greater than HEW or HEW<3000 Da. The results obtained suggest that the studied products could be used as a functional food with potential therapeutic benefit in the prevention and treatment of hypertension. This study was supported by an INIA-MCYT project (CAL01-046-02) and an MCYT project (AGL2000-1480) 2021-12-20T08:10:02Z 2021-12-20T08:10:02Z 2005-11 2021-12-20T08:10:03Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1079/BJN20051570 issn: 0007-1145 e-issn: 1475-2662 British Journal of Nutrition 94(5): 731-737 (2005) http://hdl.handle.net/10261/256447 10.1079/BJN20051570 http://dx.doi.org/10.13039/501100006280 #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement//AGL2000-1480 http://dx.doi.org/10.1079/BJN20051570 Sí none Cambridge University Press Nutrition Society (Great Britain) |
institution |
CIAL ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cial-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del CIAL España |
topic |
Hypertension Angiotensin-converting enzyme Bioactive peptides Spontaneously hypertensive rats Hypertension Angiotensin-converting enzyme Bioactive peptides Spontaneously hypertensive rats |
spellingShingle |
Hypertension Angiotensin-converting enzyme Bioactive peptides Spontaneously hypertensive rats Hypertension Angiotensin-converting enzyme Bioactive peptides Spontaneously hypertensive rats Miguel, Marta López-Fandiño, Rosina Ramos, Mercedes Aleixandre, Amaya Short-term effect of egg-white hydrolysate products on the arterial blood pressure of hypertensive rats |
description |
In the present study we evaluate the blood pressure-lowering effect of the following products: the hydrolysate obtained from egg white (EW) by enzymatic treatment with pepsin (HEW), the peptide fraction of HEW with molecular mass lower than 3000 Da (HEW<3000 Da), and three peptide sequences isolated from HEW<3000 Da (Tyr-Ala-Glu-Glu-Arg-Tyr-Pro-Ile-Leu: YAEERYPIL); (Arg-Ala-Asp-His-Pro-Phe-Leu: RADHPFL); and (Ile-Val-Phe (IVF)). These peptides, and also HEW and HEW<3000 Da, had been characterized previously in vitro as potent inhibitors of angiotensin-converting enzyme (ACE). EW and the products mentioned earlier were orally administered by gastric intubation, to 17–20-week-old male spontaneously hypertensive rats (SHR) and normotensive Wistar–Kyoto (WKY) rats. We measured the systolic blood pressure (SBP) and the diastolic blood pressure (DBP) of the rats by the tail cuff method before administration and also 2, 4, 6, 8 and 24h post-administration. Distilled water served as negative control, and we used captopril (50mg/kg) as positive control to carry out similar experiments with a known ACE inhibitor. HEW, HEW<3000 Da and the three peptide sequences decreased SBP and DBP in SHR but they did not modify these variables in WKY rats. The peptide sequences YAEERYPIL, RADHPFL and IVF showed a potency to decrease blood pressure greater than HEW or HEW<3000 Da. The results obtained suggest that the studied products could be used as a functional food with potential therapeutic benefit in the prevention and treatment of hypertension. |
author2 |
CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) |
author_facet |
CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) Miguel, Marta López-Fandiño, Rosina Ramos, Mercedes Aleixandre, Amaya |
format |
artículo |
topic_facet |
Hypertension Angiotensin-converting enzyme Bioactive peptides Spontaneously hypertensive rats |
author |
Miguel, Marta López-Fandiño, Rosina Ramos, Mercedes Aleixandre, Amaya |
author_sort |
Miguel, Marta |
title |
Short-term effect of egg-white hydrolysate products on the arterial blood pressure of hypertensive rats |
title_short |
Short-term effect of egg-white hydrolysate products on the arterial blood pressure of hypertensive rats |
title_full |
Short-term effect of egg-white hydrolysate products on the arterial blood pressure of hypertensive rats |
title_fullStr |
Short-term effect of egg-white hydrolysate products on the arterial blood pressure of hypertensive rats |
title_full_unstemmed |
Short-term effect of egg-white hydrolysate products on the arterial blood pressure of hypertensive rats |
title_sort |
short-term effect of egg-white hydrolysate products on the arterial blood pressure of hypertensive rats |
publisher |
Cambridge University Press |
publishDate |
2005-11 |
url |
http://hdl.handle.net/10261/256447 http://dx.doi.org/10.13039/501100006280 |
work_keys_str_mv |
AT miguelmarta shorttermeffectofeggwhitehydrolysateproductsonthearterialbloodpressureofhypertensiverats AT lopezfandinorosina shorttermeffectofeggwhitehydrolysateproductsonthearterialbloodpressureofhypertensiverats AT ramosmercedes shorttermeffectofeggwhitehydrolysateproductsonthearterialbloodpressureofhypertensiverats AT aleixandreamaya shorttermeffectofeggwhitehydrolysateproductsonthearterialbloodpressureofhypertensiverats |
_version_ |
1777671511677599744 |