Enzymatic preparation of structured triacylglycerides containing γ-linolenic acid

Structured triacylglycerides (STAG) rich in γ-linolenic acid (GLA) were prepared using a three-step process. First, with the addition of molecular sieves (MS) at different addition times (3, 9, 12 and 15 h) and loads (2.5, 5, 10 and 15%, based on the total weight of substrates), the enrichment of GLA from evening primrose oil (EPO) and 1-butanol (BtOH) was improved via Candida rugosa lipase-catalysed esterification reactions. Secondly, the GLA-enriched fraction was separated by thin-layer chromatography (TLC) to be further set to react during the third step in the presence of glycerol and Candida antarctica fraction B (CALB), under different enzyme loadings (5, 10, 15 and 20%, based on the total weight of substrates), temperatures (30, 40, 50 and 60 °C) and substrates molar ratios (1:1, 2:1, 3:1 and 4:1, GLA:glycerol). 60% of STAG containing 49 wt% of GLA were produced by using 15% of CALB at 60 °C and a 3:1 molar ratio.

Bibliographic Details

Main Authors:	López-Martínez, J. E., Romero, Rubi, Natividad, Reyna, Torres, Carlos F., García, Hugo S., Baeza-Jiménez, R.
Other Authors:	Consejo Nacional de Ciencia y Tecnología (México)
Format:	artículo biblioteca
Language:	English
Published:	Elsevier 2020
Online Access:	http://hdl.handle.net/10261/228629 http://dx.doi.org/10.13039/501100003141
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