In vitro permeability of saponins and sapogenins from seed extracts by the parallel artificial membrane permeability assay: Effect of in vitro gastrointestinal digestion
The permeability of saponins and sapogenins from fenugreek and quinoa extracts, as well as dioscin and diosgenin, was evaluated by the parallel artificial membrane permeability assay (PAMPA). The effect of the digestion process on permeability was determined, with previous development of a gastrointestinal process coupled to PAMPA. Saponins from both seeds displayed a moderate-to-poor permeability (>1 × 10–6 cm/s), although the digestion enhanced their permeability values in the order of 10–5 cm/s (p < 0.001). Sapogenins exhibited a similar permeability to that of saponins, although the digestion enhanced the permeability of sapogenins from quinoa (1.14 ± 0.47 × 10–5 cm/s) but not from fenugreek (2.33 ± 0.99 × 10–6 cm/s). An overall positive impact of coexisting lipids on the permeability was evidenced. PAMPA is shown as a useful, rapid, and easy tool for assessing the permeability of bioactive compounds from complex matrices, with the previous gastrointestinal process being a relevant step.
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | artículo biblioteca |
| Language: | English |
| Published: |
American Chemical Society
2020
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| Subjects: | PAMPA, Gastrointestinal digestion, Saponins, Fenugreek, Quinoa, |
| Online Access: | http://hdl.handle.net/10261/220147 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100003176 |
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| Summary: | The permeability of saponins and sapogenins from fenugreek and quinoa extracts, as well as dioscin and diosgenin, was evaluated by the parallel artificial membrane permeability assay (PAMPA). The effect of the digestion process on permeability was determined, with previous development of a gastrointestinal process coupled to PAMPA. Saponins from both seeds displayed a moderate-to-poor permeability (>1 × 10–6 cm/s), although the digestion enhanced their permeability values in the order of 10–5 cm/s (p < 0.001). Sapogenins exhibited a similar permeability to that of saponins, although the digestion enhanced the permeability of sapogenins from quinoa (1.14 ± 0.47 × 10–5 cm/s) but not from fenugreek (2.33 ± 0.99 × 10–6 cm/s). An overall positive impact of coexisting lipids on the permeability was evidenced. PAMPA is shown as a useful, rapid, and easy tool for assessing the permeability of bioactive compounds from complex matrices, with the previous gastrointestinal process being a relevant step. |
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