Different covalent immobilizations modulate lipase activities of Hypocrea pseudokoningii

Pereira, Marita G.; Velasco-Lozano, Susana; Moreno-Pérez, Sonia; Polizeli, Aline M.; Heinen, Paulo R.; Facchini, Fernanda D. A.; Vici, Ana Claudia; Cereia, Mariana; Pessela, Benevides C.; Fernández-Lorente, Gloria; Guisán, José Manuel; Jorge, João A.; Polizeli, Maria de Lourdes T. M.; Fundações de Amparo à Pesquisa (Brasil)

Different covalent immobilizations modulate lipase activities of Hypocrea pseudokoningii

This article belongs to the Special Issue Lipases and Lipases Modification.

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Main Authors:	Pereira, Marita G., Velasco-Lozano, Susana, Moreno-Pérez, Sonia, Polizeli, Aline M., Heinen, Paulo R., Facchini, Fernanda D. A., Vici, Ana Claudia, Cereia, Mariana, Pessela, Benevides C., Fernández-Lorente, Gloria, Guisán, José Manuel, Jorge, João A., Polizeli, Maria de Lourdes T. M.
Other Authors:	Fundações de Amparo à Pesquisa (Brasil)
Format:	artículo biblioteca
Language:	English
Published:	Multidisciplinary Digital Publishing Institute 2017
Subjects:	Hypocrea pseudokoningii, Enzyme immobilization, Hydrolysis of oils, Lipase activity modulation, Stability,
Online Access:	http://hdl.handle.net/10261/194046 http://dx.doi.org/10.13039/501100001807 http://dx.doi.org/10.13039/501100003593
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spelling	dig-cial-es-10261-1940462020-01-15T09:26:30Z Different covalent immobilizations modulate lipase activities of Hypocrea pseudokoningii Pereira, Marita G. Velasco-Lozano, Susana Moreno-Pérez, Sonia Polizeli, Aline M. Heinen, Paulo R. Facchini, Fernanda D. A. Vici, Ana Claudia Cereia, Mariana Pessela, Benevides C. Fernández-Lorente, Gloria Guisán, José Manuel Jorge, João A. Polizeli, Maria de Lourdes T. M. Fundações de Amparo à Pesquisa (Brasil) Fundação de Amparo à Pesquisa do Estado de São Paulo Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil) Guisán, José Manuel [0000-0003-1627-6522] Polizeli, Maria de Lourdes T. M. [0000-0002-5026-6363] Hypocrea pseudokoningii Enzyme immobilization Hydrolysis of oils Lipase activity modulation Stability This article belongs to the Special Issue Lipases and Lipases Modification. Enzyme immobilization can promote several advantages for their industrial application. In this work, a lipase from Hypocrea pseudokoningii was efficiently linked to four chemical supports: agarose activated with cyanogen bromide (CNBr), glyoxyl-agarose (GX), MANAE-agarose activated with glutaraldehyde (GA) and GA-crosslinked with glutaraldehyde. Results showed a more stable lipase with both the GA-crosslinked and GA derivatives, compared to the control (CNBr), at 50 °C, 60 °C and 70 °C. Moreover, all derivatives were stabilized when incubated with organic solvents at 50%, such as ethanol, methanol, n-propanol and cyclohexane. Furthermore, lipase was highly activated (4-fold) in the presence of cyclohexane. GA-crosslinked and GA derivatives were more stable than the CNBr one in the presence of organic solvents. All derivatives were able to hydrolyze sardine, açaí (Euterpe oleracea), cotton seed and grape seed oils. However, during the hydrolysis of sardine oil, GX derivative showed to be 2.3-fold more selectivity (eicosapentaenoic acid (EPA)/docosahexaenoic acid (DHA) ratio) than the control. Additionally, the types of immobilization interfered with the lipase enantiomeric preference. Unlike the control, the other three derivatives preferably hydrolyzed the R-isomer of 2-hydroxy-4-phenylbutanoic acid ethyl ester and the S-isomer of 1-phenylethanol acetate racemic mixtures. On the other hand, GX and CNBr derivatives preferably hydrolyzed the S-isomer of butyryl-2-phenylacetic acid racemic mixture while the GA and GA-crosslink derivatives preferably hydrolyzed the R-isomer. However, all derivatives, including the control, preferably hydrolyzed the methyl mandelate S-isomer. Moreover, the derivatives could be used for eight consecutive cycles retaining more than 50% of their residual activity. This work shows the importance of immobilization as a tool to increase the lipase stability to temperature and organic solvents, thus enabling the possibility of their application at large scale processes. This work was supported by grants from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, process No. 2013/50892-5; 2010/52322-3), Conselho de Desenvolvimento Científico e Tecnológico (CNPq process No. 406838/2013-5; 563260/2010-6). This project is also part of the National Institute of Science and Technology of the Bioethanol (No. 574002/2008-1), CNPq—Ciência sem Fronteira (No. 242775/2012-8). JAJ and MLTMP are Research Fellows of CNPq. MGP and ACV are supported by CNPq. FDAF was recipient of a FAPESP Fellowship. Peer reviewed 2019-11-06T09:50:57Z 2019-11-06T09:50:57Z 2017 artículo http://purl.org/coar/resource_type/c_6501 Molecules 22(9): 1448 (2017) http://hdl.handle.net/10261/194046 10.3390/molecules22091448 1420-3049 http://dx.doi.org/10.13039/501100001807 http://dx.doi.org/10.13039/501100003593 28869529 en Publisher's version https://doi.org/10.3390/molecules22091448 Sí open Multidisciplinary Digital Publishing Institute
institution	CIAL ES
collection	DSpace
country	España
countrycode	ES
component	Bibliográfico
access	En linea
databasecode	dig-cial-es
tag	biblioteca
region	Europa del Sur
libraryname	Biblioteca del CIAL España
language	English
topic	Hypocrea pseudokoningii Enzyme immobilization Hydrolysis of oils Lipase activity modulation Stability Hypocrea pseudokoningii Enzyme immobilization Hydrolysis of oils Lipase activity modulation Stability
spellingShingle	Hypocrea pseudokoningii Enzyme immobilization Hydrolysis of oils Lipase activity modulation Stability Hypocrea pseudokoningii Enzyme immobilization Hydrolysis of oils Lipase activity modulation Stability Pereira, Marita G. Velasco-Lozano, Susana Moreno-Pérez, Sonia Polizeli, Aline M. Heinen, Paulo R. Facchini, Fernanda D. A. Vici, Ana Claudia Cereia, Mariana Pessela, Benevides C. Fernández-Lorente, Gloria Guisán, José Manuel Jorge, João A. Polizeli, Maria de Lourdes T. M. Different covalent immobilizations modulate lipase activities of Hypocrea pseudokoningii
description	This article belongs to the Special Issue Lipases and Lipases Modification.
author2	Fundações de Amparo à Pesquisa (Brasil)
author_facet	Fundações de Amparo à Pesquisa (Brasil) Pereira, Marita G. Velasco-Lozano, Susana Moreno-Pérez, Sonia Polizeli, Aline M. Heinen, Paulo R. Facchini, Fernanda D. A. Vici, Ana Claudia Cereia, Mariana Pessela, Benevides C. Fernández-Lorente, Gloria Guisán, José Manuel Jorge, João A. Polizeli, Maria de Lourdes T. M.
format	artículo
topic_facet	Hypocrea pseudokoningii Enzyme immobilization Hydrolysis of oils Lipase activity modulation Stability
author	Pereira, Marita G. Velasco-Lozano, Susana Moreno-Pérez, Sonia Polizeli, Aline M. Heinen, Paulo R. Facchini, Fernanda D. A. Vici, Ana Claudia Cereia, Mariana Pessela, Benevides C. Fernández-Lorente, Gloria Guisán, José Manuel Jorge, João A. Polizeli, Maria de Lourdes T. M.
author_sort	Pereira, Marita G.
title	Different covalent immobilizations modulate lipase activities of Hypocrea pseudokoningii
title_short	Different covalent immobilizations modulate lipase activities of Hypocrea pseudokoningii
title_full	Different covalent immobilizations modulate lipase activities of Hypocrea pseudokoningii
title_fullStr	Different covalent immobilizations modulate lipase activities of Hypocrea pseudokoningii
title_full_unstemmed	Different covalent immobilizations modulate lipase activities of Hypocrea pseudokoningii
title_sort	different covalent immobilizations modulate lipase activities of hypocrea pseudokoningii
publisher	Multidisciplinary Digital Publishing Institute
publishDate	2017
url	http://hdl.handle.net/10261/194046 http://dx.doi.org/10.13039/501100001807 http://dx.doi.org/10.13039/501100003593
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Different covalent immobilizations modulate lipase activities of Hypocrea pseudokoningii

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