Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies
[Background] Before introducing proteins from new or alternative dietary sources into the market, a compressive risk assessment including food allergic sensitization should be carried out in order to ensure their safety. We have recently proposed the adverse outcome pathway (AOP) concept to structure the current mechanistic understanding of the molecular and cellular pathways evidenced to drive IgE-mediated food allergies. This AOP framework offers the biological context to collect and structure existing in vitro methods and to identify missing assays to evaluate sensitizing potential of food proteins. [Scope and approach] In this review, we provide a state-of-the-art overview of available in vitro approaches for assessing the sensitizing potential of food proteins, including their strengths and limitations. These approaches are structured by their potential to evaluate the molecular initiating and key events driving food sensitization. [Key findings and conclusions] The application of the AOP framework offers the opportunity to anchor existing testing methods to specific building blocks of the AOP for food sensitization. In general, in vitro methods evaluating mechanisms involved in the innate immune response are easier to address than assays addressing the adaptive immune response due to the low precursor frequency of allergen-specific T and B cells. Novel ex vivo culture strategies may have the potential to become useful tools for investigating the sensitizing potential of food proteins. When applied in the context of an integrated testing strategy, the described approaches may reduce, if not replace, current animal testing approaches.
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Elsevier
2019
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Subjects: | Adverse outcome pathway, T and B cells, Dendritic cells, Epithelial cells, IgE-mediated food allergy, In vitro models, |
Online Access: | http://hdl.handle.net/10261/193000 http://dx.doi.org/10.13039/501100000780 |
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dig-cial-es-10261-1930002022-04-20T10:06:56Z Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies Lozano-Ojalvo, Daniel Benedé, Sara Antunes, Celia M. Bavaro, Simona L. Bouchaud, Grégory Costa, Ana Denery-Papini, Sandra Díaz-Perales, Araceli Garrido-Arandia, María Gavrovic-Jankulovic, Marija Hayen, Simone Martínez-Blanco, Mónica Molina, Elena Monaci, Linda Pieters, Raymond H. H. Villemin, Clelia Wichers, Harry J. Wróblewska, Barbara Willemsen, Linette E. M. Roggen, Erwin L. Bilsen, Jolanda H. M. van European Commission Adverse outcome pathway T and B cells Dendritic cells Epithelial cells IgE-mediated food allergy In vitro models [Background] Before introducing proteins from new or alternative dietary sources into the market, a compressive risk assessment including food allergic sensitization should be carried out in order to ensure their safety. We have recently proposed the adverse outcome pathway (AOP) concept to structure the current mechanistic understanding of the molecular and cellular pathways evidenced to drive IgE-mediated food allergies. This AOP framework offers the biological context to collect and structure existing in vitro methods and to identify missing assays to evaluate sensitizing potential of food proteins. [Scope and approach] In this review, we provide a state-of-the-art overview of available in vitro approaches for assessing the sensitizing potential of food proteins, including their strengths and limitations. These approaches are structured by their potential to evaluate the molecular initiating and key events driving food sensitization. [Key findings and conclusions] The application of the AOP framework offers the opportunity to anchor existing testing methods to specific building blocks of the AOP for food sensitization. In general, in vitro methods evaluating mechanisms involved in the innate immune response are easier to address than assays addressing the adaptive immune response due to the low precursor frequency of allergen-specific T and B cells. Novel ex vivo culture strategies may have the potential to become useful tools for investigating the sensitizing potential of food proteins. When applied in the context of an integrated testing strategy, the described approaches may reduce, if not replace, current animal testing approaches. The authors are all part of the COST Action FA1402 entitled: Improving Allergy Risk Assessment Strategy for New Food Proteins (ImpARAS). Peer Reviewed 2019-10-21T11:25:21Z 2019-10-21T11:25:21Z 2019 2019-10-21T11:25:22Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1016/j.tifs.2019.01.014 issn: 0924-2244 e-issn: 1879-3053 Trends in Food Science and Technology 85: 307-319 (2019) http://hdl.handle.net/10261/193000 10.1016/j.tifs.2019.01.014 http://dx.doi.org/10.13039/501100000780 Sí none Elsevier |
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Adverse outcome pathway T and B cells Dendritic cells Epithelial cells IgE-mediated food allergy In vitro models Adverse outcome pathway T and B cells Dendritic cells Epithelial cells IgE-mediated food allergy In vitro models |
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Adverse outcome pathway T and B cells Dendritic cells Epithelial cells IgE-mediated food allergy In vitro models Adverse outcome pathway T and B cells Dendritic cells Epithelial cells IgE-mediated food allergy In vitro models Lozano-Ojalvo, Daniel Benedé, Sara Antunes, Celia M. Bavaro, Simona L. Bouchaud, Grégory Costa, Ana Denery-Papini, Sandra Díaz-Perales, Araceli Garrido-Arandia, María Gavrovic-Jankulovic, Marija Hayen, Simone Martínez-Blanco, Mónica Molina, Elena Monaci, Linda Pieters, Raymond H. H. Villemin, Clelia Wichers, Harry J. Wróblewska, Barbara Willemsen, Linette E. M. Roggen, Erwin L. Bilsen, Jolanda H. M. van Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies |
description |
[Background] Before introducing proteins from new or alternative dietary sources into the market, a compressive risk assessment including food allergic sensitization should be carried out in order to ensure their safety. We have recently proposed the adverse outcome pathway (AOP) concept to structure the current mechanistic understanding of the molecular and cellular pathways evidenced to drive IgE-mediated food allergies. This AOP framework offers the biological context to collect and structure existing in vitro methods and to identify missing assays to evaluate sensitizing potential of food proteins. [Scope and approach] In this review, we provide a state-of-the-art overview of available in vitro approaches for assessing the sensitizing potential of food proteins, including their strengths and limitations. These approaches are structured by their potential to evaluate the molecular initiating and key events driving food sensitization. [Key findings and conclusions] The application of the AOP framework offers the opportunity to anchor existing testing methods to specific building blocks of the AOP for food sensitization. In general, in vitro methods evaluating mechanisms involved in the innate immune response are easier to address than assays addressing the adaptive immune response due to the low precursor frequency of allergen-specific T and B cells. Novel ex vivo culture strategies may have the potential to become useful tools for investigating the sensitizing potential of food proteins. When applied in the context of an integrated testing strategy, the described approaches may reduce, if not replace, current animal testing approaches. |
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European Commission |
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European Commission Lozano-Ojalvo, Daniel Benedé, Sara Antunes, Celia M. Bavaro, Simona L. Bouchaud, Grégory Costa, Ana Denery-Papini, Sandra Díaz-Perales, Araceli Garrido-Arandia, María Gavrovic-Jankulovic, Marija Hayen, Simone Martínez-Blanco, Mónica Molina, Elena Monaci, Linda Pieters, Raymond H. H. Villemin, Clelia Wichers, Harry J. Wróblewska, Barbara Willemsen, Linette E. M. Roggen, Erwin L. Bilsen, Jolanda H. M. van |
format |
artículo |
topic_facet |
Adverse outcome pathway T and B cells Dendritic cells Epithelial cells IgE-mediated food allergy In vitro models |
author |
Lozano-Ojalvo, Daniel Benedé, Sara Antunes, Celia M. Bavaro, Simona L. Bouchaud, Grégory Costa, Ana Denery-Papini, Sandra Díaz-Perales, Araceli Garrido-Arandia, María Gavrovic-Jankulovic, Marija Hayen, Simone Martínez-Blanco, Mónica Molina, Elena Monaci, Linda Pieters, Raymond H. H. Villemin, Clelia Wichers, Harry J. Wróblewska, Barbara Willemsen, Linette E. M. Roggen, Erwin L. Bilsen, Jolanda H. M. van |
author_sort |
Lozano-Ojalvo, Daniel |
title |
Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies |
title_short |
Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies |
title_full |
Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies |
title_fullStr |
Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies |
title_full_unstemmed |
Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies |
title_sort |
applying the adverse outcome pathway (aop) for food sensitization to support in vitro testing strategies |
publisher |
Elsevier |
publishDate |
2019 |
url |
http://hdl.handle.net/10261/193000 http://dx.doi.org/10.13039/501100000780 |
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