Polyphenolic composition and antioxidant activity of aqueous and ethanolic extracts from Uncaria tomentosa bark and leaves
Uncaria tomentosa constitutes an important source of secondary metabolites with diverse biological activities mainly attributed until recently to alkaloids and triterpenes. We have previously reported for the first-time the polyphenolic profile of extracts from U. tomentosa, using a multi-step process involving organic solvents, as well as their antioxidant capacity, antimicrobial activity on aerial bacteria, and cytotoxicity on cancer cell lines. These promising results prompted the present study using food grade solvents suitable for the elaboration of commercial extracts. We report a detailed study on the polyphenolic composition of aqueous and ethanolic extracts of U. tomentosa bark and leaves (n = 16), using High Performance Liquid Chromatography coupled with Mass Spectrometry (HPLC-DAD/TQ-ESI-MS). A total of 32 compounds were identified, including hydroxybenzoic and hydroxycinnamic acids, flavan-3-ols monomers, procyanidin dimers and trimers, flavalignans–cinchonains and propelargonidin dimers. Our findings showed that the leaves were the richest source of total phenolics and proanthocyanidins, in particular propelargonidin dimers. Two-way Analysis of Variance (ANOVA) indicated that the contents of procyanidin and propelargonidin dimers were significantly different (p < 0.05) in function of the plant part, and leaves extracts showed higher contents. Oxygen Radical Absorbance Capacity (ORAC) and 2,2-diphenyl-1-picrylhidrazyl (DPPH) values indicated higher antioxidant capacity for the leaves (p < 0.05). Further, correlation between both methods and procyanidin dimers was found, particularly between ORAC and propelargonidin dimers. Finally, Principal Component Analysis (PCA) analysis results clearly indicated that the leaves are the richest plant part in proanthocyanidins and a very homogenous material, regardless of their origin. Therefore, our findings revealed that both ethanol and water extraction processes are adequate for the elaboration of potential commercial extracts from U. tomentosa leaves rich in proanthocyanidins and exhibiting high antioxidant activity.
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2018-05-11
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dig-cial-es-10261-1650672019-10-16T12:30:34Z Polyphenolic composition and antioxidant activity of aqueous and ethanolic extracts from Uncaria tomentosa bark and leaves Navarro-Hoyos, Mirtha Alvarado-Corella, Diego Moreira-González, Ileana Arnaez-Serrano, Elizabeth Monagas Juan, María Josefina Ministerio de Asuntos Exteriores y Cooperación (España) Consejo Superior de Investigaciones Científicas (España) Uncaria tomentosa constitutes an important source of secondary metabolites with diverse biological activities mainly attributed until recently to alkaloids and triterpenes. We have previously reported for the first-time the polyphenolic profile of extracts from U. tomentosa, using a multi-step process involving organic solvents, as well as their antioxidant capacity, antimicrobial activity on aerial bacteria, and cytotoxicity on cancer cell lines. These promising results prompted the present study using food grade solvents suitable for the elaboration of commercial extracts. We report a detailed study on the polyphenolic composition of aqueous and ethanolic extracts of U. tomentosa bark and leaves (n = 16), using High Performance Liquid Chromatography coupled with Mass Spectrometry (HPLC-DAD/TQ-ESI-MS). A total of 32 compounds were identified, including hydroxybenzoic and hydroxycinnamic acids, flavan-3-ols monomers, procyanidin dimers and trimers, flavalignans–cinchonains and propelargonidin dimers. Our findings showed that the leaves were the richest source of total phenolics and proanthocyanidins, in particular propelargonidin dimers. Two-way Analysis of Variance (ANOVA) indicated that the contents of procyanidin and propelargonidin dimers were significantly different (p < 0.05) in function of the plant part, and leaves extracts showed higher contents. Oxygen Radical Absorbance Capacity (ORAC) and 2,2-diphenyl-1-picrylhidrazyl (DPPH) values indicated higher antioxidant capacity for the leaves (p < 0.05). Further, correlation between both methods and procyanidin dimers was found, particularly between ORAC and propelargonidin dimers. Finally, Principal Component Analysis (PCA) analysis results clearly indicated that the leaves are the richest plant part in proanthocyanidins and a very homogenous material, regardless of their origin. Therefore, our findings revealed that both ethanol and water extraction processes are adequate for the elaboration of potential commercial extracts from U. tomentosa leaves rich in proanthocyanidins and exhibiting high antioxidant activity. This project was partially funded by grant from the Spanish International Development Cooperation Agency (AECID) (Ref. A/023397/09 and A/030037/10) and a joint grant from the Costa Rica-USA Foundation (CRUSA) and the Spanish Scientific Research Council (CSIC) (Ref. CR0024). 2018-05-24T15:22:08Z 2018-05-24T15:22:08Z 2018-05-11 2018-05-24T15:22:10Z artículo http://purl.org/coar/resource_type/c_6501 Antioxidants 7(5): 65 (2018) http://hdl.handle.net/10261/165067 2076-3921 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100003767 29751684 Publisher's version https://doi.org/10.3390/antiox7050065 open Multidisciplinary Digital Publishing Institute |
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Uncaria tomentosa constitutes an important source of secondary metabolites with diverse biological activities mainly attributed until recently to alkaloids and triterpenes. We have previously reported for the first-time the polyphenolic profile of extracts from U. tomentosa, using a multi-step process involving organic solvents, as well as their antioxidant capacity, antimicrobial activity on aerial bacteria, and cytotoxicity on cancer cell lines. These promising results prompted the present study using food grade solvents suitable for the elaboration of commercial extracts. We report a detailed study on the polyphenolic composition of aqueous and ethanolic extracts of U. tomentosa bark and leaves (n = 16), using High Performance Liquid Chromatography coupled with Mass Spectrometry (HPLC-DAD/TQ-ESI-MS). A total of 32 compounds were identified, including hydroxybenzoic and hydroxycinnamic acids, flavan-3-ols monomers, procyanidin dimers and trimers, flavalignans–cinchonains and propelargonidin dimers. Our findings showed that the leaves were the richest source of total phenolics and proanthocyanidins, in particular propelargonidin dimers. Two-way Analysis of Variance (ANOVA) indicated that the contents of procyanidin and propelargonidin dimers were significantly different (p < 0.05) in function of the plant part, and leaves extracts showed higher contents. Oxygen Radical Absorbance Capacity (ORAC) and 2,2-diphenyl-1-picrylhidrazyl (DPPH) values indicated higher antioxidant capacity for the leaves (p < 0.05). Further, correlation between both methods and procyanidin dimers was found, particularly between ORAC and propelargonidin dimers. Finally, Principal Component Analysis (PCA) analysis results clearly indicated that the leaves are the richest plant part in proanthocyanidins and a very homogenous material, regardless of their origin. Therefore, our findings revealed that both ethanol and water extraction processes are adequate for the elaboration of potential commercial extracts from U. tomentosa leaves rich in proanthocyanidins and exhibiting high antioxidant activity. |
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Ministerio de Asuntos Exteriores y Cooperación (España) |
author_facet |
Ministerio de Asuntos Exteriores y Cooperación (España) Navarro-Hoyos, Mirtha Alvarado-Corella, Diego Moreira-González, Ileana Arnaez-Serrano, Elizabeth Monagas Juan, María Josefina |
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Navarro-Hoyos, Mirtha Alvarado-Corella, Diego Moreira-González, Ileana Arnaez-Serrano, Elizabeth Monagas Juan, María Josefina |
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Navarro-Hoyos, Mirtha Alvarado-Corella, Diego Moreira-González, Ileana Arnaez-Serrano, Elizabeth Monagas Juan, María Josefina Polyphenolic composition and antioxidant activity of aqueous and ethanolic extracts from Uncaria tomentosa bark and leaves |
author_sort |
Navarro-Hoyos, Mirtha |
title |
Polyphenolic composition and antioxidant activity of aqueous and ethanolic extracts from Uncaria tomentosa bark and leaves |
title_short |
Polyphenolic composition and antioxidant activity of aqueous and ethanolic extracts from Uncaria tomentosa bark and leaves |
title_full |
Polyphenolic composition and antioxidant activity of aqueous and ethanolic extracts from Uncaria tomentosa bark and leaves |
title_fullStr |
Polyphenolic composition and antioxidant activity of aqueous and ethanolic extracts from Uncaria tomentosa bark and leaves |
title_full_unstemmed |
Polyphenolic composition and antioxidant activity of aqueous and ethanolic extracts from Uncaria tomentosa bark and leaves |
title_sort |
polyphenolic composition and antioxidant activity of aqueous and ethanolic extracts from uncaria tomentosa bark and leaves |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2018-05-11 |
url |
http://hdl.handle.net/10261/165067 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100003767 |
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