Hypoallergenic hydrolysates of egg white proteins modulate allergen responses induced ex vivo on spleen cells from sensitized mice
This study describes the in vivo allergenicity of enzymatic hydrolysates of egg white proteins (ovalbumin, lysozyme and ovomucoid) and explores the possibility that they could modulate T cell cytokine responses to egg allergens ex vivo, using splenocytes from BALB/c mice sensitized to individual egg proteins or to their mixtures in different proportions. The hydrolysate of ovalbumin with pepsin could be regarded as a good candidate for peptide-based immunotherapy on the grounds of its reduced ability to trigger allergic symptoms in a passive cutaneous anaphylaxis assay and its potential to reduce Th2 responses (release f IL-4 and IL-5) induced by egg allergens in the spleen cell cultures, but also to enhance Th1 responses (release of TNF-¿ and IFN-¿). While it is possible to obtain chromatographic fractions containing peptides with different Th2-inhibiting or promoting properties, as judged by cytokine production, selective peptide enrichment did not lead to an increase in the immunomodulating efficiency as compared with thewhole ovalbumin hydrolysate, possibly due to the presence in the latter of a combination of immunogenic peptides with synergistic or adjuvant actions.
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Elsevier
2016
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Subjects: | Food allergy, Hypoallergenic egg white hydrolysates, Th1 and Th2 cytokines, BALB/c mice, Immunomodulatory peptides, |
Online Access: | http://hdl.handle.net/10261/150253 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003176 |
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dig-cial-es-10261-1502532018-09-20T07:41:26Z Hypoallergenic hydrolysates of egg white proteins modulate allergen responses induced ex vivo on spleen cells from sensitized mice Lozano-Ojalvo, Daniel Molina, Elena López-Fandiño, Rosina European Commission Ministerio de Educación, Cultura y Deporte (España) Ministerio de Economía y Competitividad (España) Food allergy Hypoallergenic egg white hydrolysates Th1 and Th2 cytokines BALB/c mice Immunomodulatory peptides This study describes the in vivo allergenicity of enzymatic hydrolysates of egg white proteins (ovalbumin, lysozyme and ovomucoid) and explores the possibility that they could modulate T cell cytokine responses to egg allergens ex vivo, using splenocytes from BALB/c mice sensitized to individual egg proteins or to their mixtures in different proportions. The hydrolysate of ovalbumin with pepsin could be regarded as a good candidate for peptide-based immunotherapy on the grounds of its reduced ability to trigger allergic symptoms in a passive cutaneous anaphylaxis assay and its potential to reduce Th2 responses (release f IL-4 and IL-5) induced by egg allergens in the spleen cell cultures, but also to enhance Th1 responses (release of TNF-¿ and IFN-¿). While it is possible to obtain chromatographic fractions containing peptides with different Th2-inhibiting or promoting properties, as judged by cytokine production, selective peptide enrichment did not lead to an increase in the immunomodulating efficiency as compared with thewhole ovalbumin hydrolysate, possibly due to the presence in the latter of a combination of immunogenic peptides with synergistic or adjuvant actions. Financial support was received from the project AGL2014-59771-R from MINECO (Spain). The authors are participants in the COST-Action ImpARAS FA1402. D. L-O is recipient of an FPU fellowship from MECD (Spain). Peer Reviewed 2017-05-24T09:59:21Z 2017-05-24T09:59:21Z 2016 2017-05-24T09:59:22Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1016/j.foodres.2016.09.021 issn: 0963-9969 e-issn: 1873-7145 Food Research International 89(1): 661-669 (2016) http://hdl.handle.net/10261/150253 10.1016/j.foodres.2016.09.021 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003176 #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-59771-R Sí none Elsevier |
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Food allergy Hypoallergenic egg white hydrolysates Th1 and Th2 cytokines BALB/c mice Immunomodulatory peptides Food allergy Hypoallergenic egg white hydrolysates Th1 and Th2 cytokines BALB/c mice Immunomodulatory peptides |
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Food allergy Hypoallergenic egg white hydrolysates Th1 and Th2 cytokines BALB/c mice Immunomodulatory peptides Food allergy Hypoallergenic egg white hydrolysates Th1 and Th2 cytokines BALB/c mice Immunomodulatory peptides Lozano-Ojalvo, Daniel Molina, Elena López-Fandiño, Rosina Hypoallergenic hydrolysates of egg white proteins modulate allergen responses induced ex vivo on spleen cells from sensitized mice |
description |
This study describes the in vivo allergenicity of enzymatic hydrolysates of egg white proteins (ovalbumin, lysozyme and ovomucoid) and explores the possibility that they could modulate T cell cytokine responses to egg allergens ex vivo, using splenocytes from BALB/c mice sensitized to individual egg proteins or to their mixtures in different proportions. The hydrolysate of ovalbumin with pepsin could be regarded as a good candidate for peptide-based immunotherapy on the grounds of its reduced ability to trigger allergic symptoms in a passive cutaneous anaphylaxis assay and its potential to reduce Th2 responses (release f IL-4 and IL-5) induced by egg allergens in the spleen cell cultures, but also to enhance Th1 responses (release of TNF-¿ and IFN-¿). While it is possible to obtain chromatographic fractions containing peptides with different Th2-inhibiting or promoting properties, as judged by cytokine production, selective peptide enrichment did not lead to an increase in the immunomodulating efficiency as compared with thewhole ovalbumin hydrolysate, possibly due to the presence in the latter of a combination of immunogenic peptides with synergistic or adjuvant actions. |
author2 |
European Commission |
author_facet |
European Commission Lozano-Ojalvo, Daniel Molina, Elena López-Fandiño, Rosina |
format |
artículo |
topic_facet |
Food allergy Hypoallergenic egg white hydrolysates Th1 and Th2 cytokines BALB/c mice Immunomodulatory peptides |
author |
Lozano-Ojalvo, Daniel Molina, Elena López-Fandiño, Rosina |
author_sort |
Lozano-Ojalvo, Daniel |
title |
Hypoallergenic hydrolysates of egg white proteins modulate allergen responses induced ex vivo on spleen cells from sensitized mice |
title_short |
Hypoallergenic hydrolysates of egg white proteins modulate allergen responses induced ex vivo on spleen cells from sensitized mice |
title_full |
Hypoallergenic hydrolysates of egg white proteins modulate allergen responses induced ex vivo on spleen cells from sensitized mice |
title_fullStr |
Hypoallergenic hydrolysates of egg white proteins modulate allergen responses induced ex vivo on spleen cells from sensitized mice |
title_full_unstemmed |
Hypoallergenic hydrolysates of egg white proteins modulate allergen responses induced ex vivo on spleen cells from sensitized mice |
title_sort |
hypoallergenic hydrolysates of egg white proteins modulate allergen responses induced ex vivo on spleen cells from sensitized mice |
publisher |
Elsevier |
publishDate |
2016 |
url |
http://hdl.handle.net/10261/150253 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003176 |
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