Hydrolysates of egg white proteins modulate T- and B-cell responses in mitogen-stimulated murine cells
This work assessed the effects of hydrolysates of ovalbumin (OVA), lysozyme (LYS), ovomucoid (OM) and whole egg white (EW) on cytokine secretion, antibody production, oxidative stress and proliferation of murine spleen and mesenteric lymph node cells stimulated with T- (concanavalin A-ConA) or B-cell mitogens (lipopolysaccharide-LPS). The hydrolysates of OVA, LYS and EW with alcalase reduced ConA-stimulated lymphocyte proliferation and production of Th2-biased cytokines, such as IL-13 and IL-10, and decreased the secretion of the Th1 cytokine TNF-α. In addition, these hydrolysates considerably inhibited IgG1-class switching induced by LPS and counteracted the release of reactive oxygen species. EW peptides modulated the immune responses of murine cells to mitogen stimuli, revealing potential activities that could be used for different purposes as Th1- or Th2-skewing mediators.
Saved in:
| Main Authors: | , , |
|---|---|
| Other Authors: | |
| Format: | artículo biblioteca |
| Published: |
Royal Society of Chemistry (UK)
2016
|
| Online Access: | http://hdl.handle.net/10261/150251 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100003176 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
dig-cial-es-10261-150251 |
|---|---|
| record_format |
koha |
| spelling |
dig-cial-es-10261-1502512018-09-20T07:41:26Z Hydrolysates of egg white proteins modulate T- and B-cell responses in mitogen-stimulated murine cells Lozano-Ojalvo, Daniel Molina, Elena López-Fandiño, Rosina Ministerio de Economía y Competitividad (España) European Commission Ministerio de Educación, Cultura y Deporte (España) This work assessed the effects of hydrolysates of ovalbumin (OVA), lysozyme (LYS), ovomucoid (OM) and whole egg white (EW) on cytokine secretion, antibody production, oxidative stress and proliferation of murine spleen and mesenteric lymph node cells stimulated with T- (concanavalin A-ConA) or B-cell mitogens (lipopolysaccharide-LPS). The hydrolysates of OVA, LYS and EW with alcalase reduced ConA-stimulated lymphocyte proliferation and production of Th2-biased cytokines, such as IL-13 and IL-10, and decreased the secretion of the Th1 cytokine TNF-α. In addition, these hydrolysates considerably inhibited IgG1-class switching induced by LPS and counteracted the release of reactive oxygen species. EW peptides modulated the immune responses of murine cells to mitogen stimuli, revealing potential activities that could be used for different purposes as Th1- or Th2-skewing mediators. Financial support was received from the project AGL2014-59771-R (MINECO, Spain). R. L-F, E. M. and D. L-O are participants in the COST-Action ImpARAS FA1402. D. L-O is a recipient of an FPU fellowship from MECD (Spain). Peer Reviewed 2017-05-24T09:45:56Z 2017-05-24T09:45:56Z 2016 2017-05-24T09:45:57Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1039/c5fo00614g e-issn: 2042-650X issn: 2042-6496 Food and Function 7(2): 1048-1056 (2016) http://hdl.handle.net/10261/150251 10.1039/c5fo00614g http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100003176 #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-59771-R Sí none Royal Society of Chemistry (UK) |
| institution |
CIAL ES |
| collection |
DSpace |
| country |
España |
| countrycode |
ES |
| component |
Bibliográfico |
| access |
En linea |
| databasecode |
dig-cial-es |
| tag |
biblioteca |
| region |
Europa del Sur |
| libraryname |
Biblioteca del CIAL España |
| description |
This work assessed the effects of hydrolysates of ovalbumin (OVA), lysozyme (LYS), ovomucoid (OM) and whole egg white (EW) on cytokine secretion, antibody production, oxidative stress and proliferation of murine spleen and mesenteric lymph node cells stimulated with T- (concanavalin A-ConA) or B-cell mitogens (lipopolysaccharide-LPS). The hydrolysates of OVA, LYS and EW with alcalase reduced ConA-stimulated lymphocyte proliferation and production of Th2-biased cytokines, such as IL-13 and IL-10, and decreased the secretion of the Th1 cytokine TNF-α. In addition, these hydrolysates considerably inhibited IgG1-class switching induced by LPS and counteracted the release of reactive oxygen species. EW peptides modulated the immune responses of murine cells to mitogen stimuli, revealing potential activities that could be used for different purposes as Th1- or Th2-skewing mediators. |
| author2 |
Ministerio de Economía y Competitividad (España) |
| author_facet |
Ministerio de Economía y Competitividad (España) Lozano-Ojalvo, Daniel Molina, Elena López-Fandiño, Rosina |
| format |
artículo |
| author |
Lozano-Ojalvo, Daniel Molina, Elena López-Fandiño, Rosina |
| spellingShingle |
Lozano-Ojalvo, Daniel Molina, Elena López-Fandiño, Rosina Hydrolysates of egg white proteins modulate T- and B-cell responses in mitogen-stimulated murine cells |
| author_sort |
Lozano-Ojalvo, Daniel |
| title |
Hydrolysates of egg white proteins modulate T- and B-cell responses in mitogen-stimulated murine cells |
| title_short |
Hydrolysates of egg white proteins modulate T- and B-cell responses in mitogen-stimulated murine cells |
| title_full |
Hydrolysates of egg white proteins modulate T- and B-cell responses in mitogen-stimulated murine cells |
| title_fullStr |
Hydrolysates of egg white proteins modulate T- and B-cell responses in mitogen-stimulated murine cells |
| title_full_unstemmed |
Hydrolysates of egg white proteins modulate T- and B-cell responses in mitogen-stimulated murine cells |
| title_sort |
hydrolysates of egg white proteins modulate t- and b-cell responses in mitogen-stimulated murine cells |
| publisher |
Royal Society of Chemistry (UK) |
| publishDate |
2016 |
| url |
http://hdl.handle.net/10261/150251 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100003176 |
| work_keys_str_mv |
AT lozanoojalvodaniel hydrolysatesofeggwhiteproteinsmodulatetandbcellresponsesinmitogenstimulatedmurinecells AT molinaelena hydrolysatesofeggwhiteproteinsmodulatetandbcellresponsesinmitogenstimulatedmurinecells AT lopezfandinorosina hydrolysatesofeggwhiteproteinsmodulatetandbcellresponsesinmitogenstimulatedmurinecells |
| _version_ |
1777671259294793728 |