Identification of an IgE reactive peptide in hen egg riboflavin binding protein subjected to simulated gastrointestinal digestion

Identification of an IgE reactive peptide in hen egg riboflavin binding protein subjected to simulated gastrointestinal digestion

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Resumen del póster presentado al 1st International Conference on Food Digestion celebrado en Cesena (Italia) del 19 al 21 de marzo de 2012.

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Main Authors: Martos, Gustavo, Pineda-Vadillo, Carlos, Miralles, Beatriz, Alonso-Lebrero, Elena, López-Fandiño, Rosina, Molina, Elena, Belloque, Josefina
Format: póster de congreso biblioteca
Published: 2012
Online Access:http://hdl.handle.net/10261/114582
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spelling dig-cial-es-10261-1145822018-09-20T07:41:26Z Identification of an IgE reactive peptide in hen egg riboflavin binding protein subjected to simulated gastrointestinal digestion Martos, Gustavo Pineda-Vadillo, Carlos Miralles, Beatriz Alonso-Lebrero, Elena López-Fandiño, Rosina Molina, Elena Belloque, Josefina Resumen del póster presentado al 1st International Conference on Food Digestion celebrado en Cesena (Italia) del 19 al 21 de marzo de 2012. Riboflavin binding protein (RfBP) is a minor protein in hen egg, whose potential involvement in egg allergy has seldom been studied. For an allergen to induce a reaction in sensitized individuals, it must survive the passage through the gastrointestinal tract in a sufficiently intact or immunologically active form. The aim of this work was to investigate the capacity of egg white RfBP to bind IgE from egg-allergic patients before and after a simulated gastrointestinal digestion. The protein was cleaved mainly in the gastric phase of digestion. After gastroduodenal digestion, the protein was fragmented but linked through disulfide bonds. The intact protein and its digests were subjected to inhibition ELlSA using sera from patients allergic to egg. The results showed a considerable IgE-binding for intact RfBP, while the digests showed a reduced but still relevant IgEbinding, which varied with the serum used. The digests were then subjected to immunoblot with allergic patients' sera, and at least two IgE-reactive peptides (of apparent molecular weight 3.9 and 5.1 kDa) were detected. These peptides were excised from the gel, tfypsinized and analyzed by MALDI-TOFKOF mass spectrometry for identification. The results showed that peptide 41-84 and 52/53-84 from RfBP could contain one or multiple IgE binding epitopes that would resist gastrointestinal digestion, being large enough to potentially crosslink IgE on mast cells and trigger an allergic reaction in sensitized individuals. Peer Reviewed 2015-04-30T12:06:26Z 2015-04-30T12:06:26Z 2012 2015-04-30T12:06:26Z póster de congreso http://purl.org/coar/resource_type/c_6670 1st ICFD (2012) http://hdl.handle.net/10261/114582 Sí none
institution CIAL ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-cial-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del CIAL España
description Resumen del póster presentado al 1st International Conference on Food Digestion celebrado en Cesena (Italia) del 19 al 21 de marzo de 2012.
format póster de congreso
author Martos, Gustavo
Pineda-Vadillo, Carlos
Miralles, Beatriz
Alonso-Lebrero, Elena
López-Fandiño, Rosina
Molina, Elena
Belloque, Josefina
spellingShingle Martos, Gustavo
Pineda-Vadillo, Carlos
Miralles, Beatriz
Alonso-Lebrero, Elena
López-Fandiño, Rosina
Molina, Elena
Belloque, Josefina
Identification of an IgE reactive peptide in hen egg riboflavin binding protein subjected to simulated gastrointestinal digestion
author_facet Martos, Gustavo
Pineda-Vadillo, Carlos
Miralles, Beatriz
Alonso-Lebrero, Elena
López-Fandiño, Rosina
Molina, Elena
Belloque, Josefina
author_sort Martos, Gustavo
title Identification of an IgE reactive peptide in hen egg riboflavin binding protein subjected to simulated gastrointestinal digestion
title_short Identification of an IgE reactive peptide in hen egg riboflavin binding protein subjected to simulated gastrointestinal digestion
title_full Identification of an IgE reactive peptide in hen egg riboflavin binding protein subjected to simulated gastrointestinal digestion
title_fullStr Identification of an IgE reactive peptide in hen egg riboflavin binding protein subjected to simulated gastrointestinal digestion
title_full_unstemmed Identification of an IgE reactive peptide in hen egg riboflavin binding protein subjected to simulated gastrointestinal digestion
title_sort identification of an ige reactive peptide in hen egg riboflavin binding protein subjected to simulated gastrointestinal digestion
publishDate 2012
url http://hdl.handle.net/10261/114582
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AT mirallesbeatriz identificationofanigereactivepeptideinheneggriboflavinbindingproteinsubjectedtosimulatedgastrointestinaldigestion
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