Recovery of betulinic acid from plane tree (Platanus acerifolia L.)
Betulinic acid (3β, hydroxy-lup-20(29)-en-28-oic acid) is a bioactive triterpenic acid which was identified in various botanical sources and in considerable amounts in the bark of plane tree (Platanus acerifolia L.). In this work, the recovery of betulinic acid from plane tree bark was studied using different liquid solvent based extraction methods, namely solid-liquid extraction (SLE), ultrasound assisted extraction (UAE) and pressurized liquid extraction (PLE). Furthermore, preliminary studies of the supercritical fluid extraction (SFE) of plane tree bark are also reported. The liquid solvent based extraction techniques (SLE, UAE and PLE) were carried out using ethanol and ethyl acetate, and produced a recovery of betulinic acid in the range 10-15 mg/g of bark, with concentrations around 25-35% mass. A betulinic acid enrichment in the ethanolic extracts was possible by means of a simple precipitation step adding water. The precipitate contained 42-46% mass of betulinic acid and high recovery (>95%). Increasing the extraction temperature, by means of the PLE assays, has not resulted in an improvement of betulinic acid recovery. The preliminary SFE assays produced lower recoveries of betulinic acid (0.5-8 mg/g) with respect to liquid extraction. The addition of ethanol as cosolvent produced a significant improvement of both betulinic acid recovery and concentration in the SFE extract.
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Elsevier
2014
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Subjects: | Ultrasound assisted extraction, Pressurized liquid extraction, Betulinic acid, Plane tree bark, Platanus acerifolia, Supercritical fluid extraction, |
Online Access: | http://hdl.handle.net/10261/113637 http://dx.doi.org/10.13039/100012818 |
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dig-cial-es-10261-1136372018-09-18T10:31:03Z Recovery of betulinic acid from plane tree (Platanus acerifolia L.) Pinilla, José María López-Padilla, Alexis Vicente, G. Fornari, Tiziana Quintela, J. C. Reglero, Guillermo Comunidad de Madrid Colciencias (Colombia) Agencia de Educación Superior de Medellín Ultrasound assisted extraction Pressurized liquid extraction Betulinic acid Plane tree bark Platanus acerifolia Supercritical fluid extraction Betulinic acid (3β, hydroxy-lup-20(29)-en-28-oic acid) is a bioactive triterpenic acid which was identified in various botanical sources and in considerable amounts in the bark of plane tree (Platanus acerifolia L.). In this work, the recovery of betulinic acid from plane tree bark was studied using different liquid solvent based extraction methods, namely solid-liquid extraction (SLE), ultrasound assisted extraction (UAE) and pressurized liquid extraction (PLE). Furthermore, preliminary studies of the supercritical fluid extraction (SFE) of plane tree bark are also reported. The liquid solvent based extraction techniques (SLE, UAE and PLE) were carried out using ethanol and ethyl acetate, and produced a recovery of betulinic acid in the range 10-15 mg/g of bark, with concentrations around 25-35% mass. A betulinic acid enrichment in the ethanolic extracts was possible by means of a simple precipitation step adding water. The precipitate contained 42-46% mass of betulinic acid and high recovery (>95%). Increasing the extraction temperature, by means of the PLE assays, has not resulted in an improvement of betulinic acid recovery. The preliminary SFE assays produced lower recoveries of betulinic acid (0.5-8 mg/g) with respect to liquid extraction. The addition of ethanol as cosolvent produced a significant improvement of both betulinic acid recovery and concentration in the SFE extract. This work has been supported by project ALIBIRD-S2009/AGR-1469 from Comunidad Autónoma de Madrid. López-Padilla A. thanks to COLCIENCIAS (568–2012) and Medellin Mayor's Office (Sapiencia/Enlaza Mundos Program, 2013) for the Ph.D. fellowship. Peer Reviewed 2015-04-14T09:58:34Z 2015-04-14T09:58:34Z 2014 2015-04-14T09:58:34Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1016/j.supflu.2014.09.001 issn: 0896-8446 e-issn: 1872-8162 Journal of Supercritical Fluids 95: 541-545 (2014) http://hdl.handle.net/10261/113637 10.1016/j.supflu.2014.09.001 http://dx.doi.org/10.13039/100012818 #PLACEHOLDER_PARENT_METADATA_VALUE# S2009/AGR-1469/ALIBIRD Postprint Postprint http://dx.doi.org/10.1016/j.supflu.2014.09.001 Sí open Elsevier |
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Ultrasound assisted extraction Pressurized liquid extraction Betulinic acid Plane tree bark Platanus acerifolia Supercritical fluid extraction Ultrasound assisted extraction Pressurized liquid extraction Betulinic acid Plane tree bark Platanus acerifolia Supercritical fluid extraction |
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Ultrasound assisted extraction Pressurized liquid extraction Betulinic acid Plane tree bark Platanus acerifolia Supercritical fluid extraction Ultrasound assisted extraction Pressurized liquid extraction Betulinic acid Plane tree bark Platanus acerifolia Supercritical fluid extraction Pinilla, José María López-Padilla, Alexis Vicente, G. Fornari, Tiziana Quintela, J. C. Reglero, Guillermo Recovery of betulinic acid from plane tree (Platanus acerifolia L.) |
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Betulinic acid (3β, hydroxy-lup-20(29)-en-28-oic acid) is a bioactive triterpenic acid which was identified in various botanical sources and in considerable amounts in the bark of plane tree (Platanus acerifolia L.). In this work, the recovery of betulinic acid from plane tree bark was studied using different liquid solvent based extraction methods, namely solid-liquid extraction (SLE), ultrasound assisted extraction (UAE) and pressurized liquid extraction (PLE). Furthermore, preliminary studies of the supercritical fluid extraction (SFE) of plane tree bark are also reported. The liquid solvent based extraction techniques (SLE, UAE and PLE) were carried out using ethanol and ethyl acetate, and produced a recovery of betulinic acid in the range 10-15 mg/g of bark, with concentrations around 25-35% mass. A betulinic acid enrichment in the ethanolic extracts was possible by means of a simple precipitation step adding water. The precipitate contained 42-46% mass of betulinic acid and high recovery (>95%). Increasing the extraction temperature, by means of the PLE assays, has not resulted in an improvement of betulinic acid recovery. The preliminary SFE assays produced lower recoveries of betulinic acid (0.5-8 mg/g) with respect to liquid extraction. The addition of ethanol as cosolvent produced a significant improvement of both betulinic acid recovery and concentration in the SFE extract. |
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Comunidad de Madrid |
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Comunidad de Madrid Pinilla, José María López-Padilla, Alexis Vicente, G. Fornari, Tiziana Quintela, J. C. Reglero, Guillermo |
format |
artículo |
topic_facet |
Ultrasound assisted extraction Pressurized liquid extraction Betulinic acid Plane tree bark Platanus acerifolia Supercritical fluid extraction |
author |
Pinilla, José María López-Padilla, Alexis Vicente, G. Fornari, Tiziana Quintela, J. C. Reglero, Guillermo |
author_sort |
Pinilla, José María |
title |
Recovery of betulinic acid from plane tree (Platanus acerifolia L.) |
title_short |
Recovery of betulinic acid from plane tree (Platanus acerifolia L.) |
title_full |
Recovery of betulinic acid from plane tree (Platanus acerifolia L.) |
title_fullStr |
Recovery of betulinic acid from plane tree (Platanus acerifolia L.) |
title_full_unstemmed |
Recovery of betulinic acid from plane tree (Platanus acerifolia L.) |
title_sort |
recovery of betulinic acid from plane tree (platanus acerifolia l.) |
publisher |
Elsevier |
publishDate |
2014 |
url |
http://hdl.handle.net/10261/113637 http://dx.doi.org/10.13039/100012818 |
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