Hybrid composites octyl-silica-methacrylate agglomerates as enzyme supports

The use of immobilized enzymes as catalysts may be limited by particle size which must be larger than the mesh that retains them in the reactor. Octyl-silica (OS) beads of 70 μm average size were agglomerated to obtain hybrid organic–inorganic composites with particle sizes between 100 and 200 μm. The agglomeration process has been achieved by polymerization of methacrylate from glycidyl methacrylate and ethylene dimethacrylate in the presence of silica beads and further functionalization of the composite with octyl groups. Methacrylate content of the composite (20%) is high enough to stick OS beads, and low enough to preserve the advantages of these particles as supports. The properties of the octyl silica particles for lipase immobilization have been very closely reproduced with the octyl-silica-methacrylate (OSM) composite. Enzyme loading of 210 mg lipase per gram of support has been achieved on OSM vs 230 mg/g on OS. Also catalytic activity values are close for both catalysts, OSM-lipase remaining fully active and stable after 15 cycles in acetonitrile.

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Bibliographic Details
Main Authors: Fernández, Óscar, Díaz Carretero, Isabel, Torres, Carlos F., Tobajas, Montserrat, Tejedor, Víctor, Blanco Martín, Rosa María
Other Authors: Ministerio de Ciencia y Tecnología (España)
Format: artículo biblioteca
Published: Elsevier 2013
Subjects:Diffusional limitations, Hybrid composites, Hydrophobic supports, Particle agglomeration, Enzyme immobilization,
Online Access:http://hdl.handle.net/10261/100046
http://dx.doi.org/10.13039/501100006280
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spelling dig-cial-es-10261-1000462018-09-18T12:13:18Z Hybrid composites octyl-silica-methacrylate agglomerates as enzyme supports Fernández, Óscar Díaz Carretero, Isabel Torres, Carlos F. Tobajas, Montserrat Tejedor, Víctor Blanco Martín, Rosa María Ministerio de Ciencia y Tecnología (España) Diffusional limitations Hybrid composites Hydrophobic supports Particle agglomeration Enzyme immobilization The use of immobilized enzymes as catalysts may be limited by particle size which must be larger than the mesh that retains them in the reactor. Octyl-silica (OS) beads of 70 μm average size were agglomerated to obtain hybrid organic–inorganic composites with particle sizes between 100 and 200 μm. The agglomeration process has been achieved by polymerization of methacrylate from glycidyl methacrylate and ethylene dimethacrylate in the presence of silica beads and further functionalization of the composite with octyl groups. Methacrylate content of the composite (20%) is high enough to stick OS beads, and low enough to preserve the advantages of these particles as supports. The properties of the octyl silica particles for lipase immobilization have been very closely reproduced with the octyl-silica-methacrylate (OSM) composite. Enzyme loading of 210 mg lipase per gram of support has been achieved on OSM vs 230 mg/g on OS. Also catalytic activity values are close for both catalysts, OSM-lipase remaining fully active and stable after 15 cycles in acetonitrile. This work has been supported by the Spanish project MAT-2009-13569. Peer Reviewed 2014-07-17T08:00:11Z 2014-07-17T08:00:11Z 2013 2014-07-17T08:00:12Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1016/j.apcata.2012.10.021 issn: 0926-860X Applied Catalysis A: General 450: 204-210 (2013) http://hdl.handle.net/10261/100046 10.1016/j.apcata.2012.10.021 http://dx.doi.org/10.13039/501100006280 none Elsevier
institution CIAL ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-cial-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del CIAL España
topic Diffusional limitations
Hybrid composites
Hydrophobic supports
Particle agglomeration
Enzyme immobilization
Diffusional limitations
Hybrid composites
Hydrophobic supports
Particle agglomeration
Enzyme immobilization
spellingShingle Diffusional limitations
Hybrid composites
Hydrophobic supports
Particle agglomeration
Enzyme immobilization
Diffusional limitations
Hybrid composites
Hydrophobic supports
Particle agglomeration
Enzyme immobilization
Fernández, Óscar
Díaz Carretero, Isabel
Torres, Carlos F.
Tobajas, Montserrat
Tejedor, Víctor
Blanco Martín, Rosa María
Hybrid composites octyl-silica-methacrylate agglomerates as enzyme supports
description The use of immobilized enzymes as catalysts may be limited by particle size which must be larger than the mesh that retains them in the reactor. Octyl-silica (OS) beads of 70 μm average size were agglomerated to obtain hybrid organic–inorganic composites with particle sizes between 100 and 200 μm. The agglomeration process has been achieved by polymerization of methacrylate from glycidyl methacrylate and ethylene dimethacrylate in the presence of silica beads and further functionalization of the composite with octyl groups. Methacrylate content of the composite (20%) is high enough to stick OS beads, and low enough to preserve the advantages of these particles as supports. The properties of the octyl silica particles for lipase immobilization have been very closely reproduced with the octyl-silica-methacrylate (OSM) composite. Enzyme loading of 210 mg lipase per gram of support has been achieved on OSM vs 230 mg/g on OS. Also catalytic activity values are close for both catalysts, OSM-lipase remaining fully active and stable after 15 cycles in acetonitrile.
author2 Ministerio de Ciencia y Tecnología (España)
author_facet Ministerio de Ciencia y Tecnología (España)
Fernández, Óscar
Díaz Carretero, Isabel
Torres, Carlos F.
Tobajas, Montserrat
Tejedor, Víctor
Blanco Martín, Rosa María
format artículo
topic_facet Diffusional limitations
Hybrid composites
Hydrophobic supports
Particle agglomeration
Enzyme immobilization
author Fernández, Óscar
Díaz Carretero, Isabel
Torres, Carlos F.
Tobajas, Montserrat
Tejedor, Víctor
Blanco Martín, Rosa María
author_sort Fernández, Óscar
title Hybrid composites octyl-silica-methacrylate agglomerates as enzyme supports
title_short Hybrid composites octyl-silica-methacrylate agglomerates as enzyme supports
title_full Hybrid composites octyl-silica-methacrylate agglomerates as enzyme supports
title_fullStr Hybrid composites octyl-silica-methacrylate agglomerates as enzyme supports
title_full_unstemmed Hybrid composites octyl-silica-methacrylate agglomerates as enzyme supports
title_sort hybrid composites octyl-silica-methacrylate agglomerates as enzyme supports
publisher Elsevier
publishDate 2013
url http://hdl.handle.net/10261/100046
http://dx.doi.org/10.13039/501100006280
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