Seed storage proteins ARL2 and its variants from the apalocus of wild tepary bean G40199 confers resistance to acanthoscellides obtectus when expressed in common beans
Wild tepary beans (Phaseolus acutifolius) contain arcelins and arcelin-like (ARL2) proteins that are co-expressed with other proteins of the arcelin, α-amylase inhibitors and phytohemagglutinins (APA) locus. Arcelin and ARL2 protein from a wild tepary bean accession G40199 were transferred into common bean cultivars ICA Pijao and Rojo. Inbred backcross lines were developed and selected for segregation of the proteins at BC2F2:3 generations. Seeds from homozygous, segregating lines expressing the arcelin and ARL2, plus those without the proteins, were used for screening against resistance to A. obtectus. Significant delay of 63 days for 50% adult emergence and reduced number of emerged F1 of A. obtectus adults were observed in backcross lines with homozygous expression of arcelin and ARL2 tepary bean proteins. Furthermore, a reduction in size and weight of emerged adult insects to almost half was observed. This work demonstrates the superior resistance common bean backcross lines to A. obtectus conferred by the presence of the APA proteins introgressed from tepary bean.
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Format: | Journal Article biblioteca |
Language: | English |
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African Crop Science Society
2011
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Subjects: | phaseolus acutifolius, arcelin, common beans, |
Online Access: | https://hdl.handle.net/10568/97068 https://www.ajol.info/index.php/acsj/article/download/74170/64829 |
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dig-cgspace-10568-970682023-06-12T08:51:58Z Seed storage proteins ARL2 and its variants from the apalocus of wild tepary bean G40199 confers resistance to acanthoscellides obtectus when expressed in common beans Kusolwa, PM Myersm, JR phaseolus acutifolius arcelin common beans Wild tepary beans (Phaseolus acutifolius) contain arcelins and arcelin-like (ARL2) proteins that are co-expressed with other proteins of the arcelin, α-amylase inhibitors and phytohemagglutinins (APA) locus. Arcelin and ARL2 protein from a wild tepary bean accession G40199 were transferred into common bean cultivars ICA Pijao and Rojo. Inbred backcross lines were developed and selected for segregation of the proteins at BC2F2:3 generations. Seeds from homozygous, segregating lines expressing the arcelin and ARL2, plus those without the proteins, were used for screening against resistance to A. obtectus. Significant delay of 63 days for 50% adult emergence and reduced number of emerged F1 of A. obtectus adults were observed in backcross lines with homozygous expression of arcelin and ARL2 tepary bean proteins. Furthermore, a reduction in size and weight of emerged adult insects to almost half was observed. This work demonstrates the superior resistance common bean backcross lines to A. obtectus conferred by the presence of the APA proteins introgressed from tepary bean. 2011 2018-09-04T15:30:10Z 2018-09-04T15:30:10Z Journal Article Kusolwa, PM; Myersm, JR. (2011). Seed storage proteins ARL2 and its variants from the apalocus of wild tepary bean G40199 confers resistance to acanthoscellides obtectus when expressed in common beans. African Crop Science Journal, 19(4): 255-265 1021-9730 https://hdl.handle.net/10568/97068 https://www.ajol.info/index.php/acsj/article/download/74170/64829 en Open Access 255-265 African Crop Science Society African Crop Science Journal |
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phaseolus acutifolius arcelin common beans phaseolus acutifolius arcelin common beans Kusolwa, PM Myersm, JR Seed storage proteins ARL2 and its variants from the apalocus of wild tepary bean G40199 confers resistance to acanthoscellides obtectus when expressed in common beans |
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Wild tepary beans (Phaseolus acutifolius) contain arcelins and arcelin-like (ARL2) proteins that are co-expressed with other proteins of the arcelin, α-amylase inhibitors and phytohemagglutinins (APA) locus. Arcelin and ARL2 protein from a wild tepary bean accession G40199 were transferred into common bean cultivars ICA Pijao and Rojo. Inbred backcross lines were developed and selected for segregation of the proteins at BC2F2:3 generations. Seeds from homozygous, segregating lines expressing the arcelin and ARL2, plus those without the proteins, were used for screening against resistance to A. obtectus. Significant delay of 63 days for 50% adult emergence and reduced number of emerged F1 of A. obtectus adults were observed in backcross lines with homozygous expression of arcelin and ARL2 tepary bean proteins. Furthermore, a reduction in size and weight of emerged adult insects to almost half was observed. This work demonstrates the superior resistance common bean backcross lines to A. obtectus conferred by the presence of the APA proteins introgressed from tepary bean. |
format |
Journal Article |
topic_facet |
phaseolus acutifolius arcelin common beans |
author |
Kusolwa, PM Myersm, JR |
author_facet |
Kusolwa, PM Myersm, JR |
author_sort |
Kusolwa, PM |
title |
Seed storage proteins ARL2 and its variants from the apalocus of wild tepary bean G40199 confers resistance to acanthoscellides obtectus when expressed in common beans |
title_short |
Seed storage proteins ARL2 and its variants from the apalocus of wild tepary bean G40199 confers resistance to acanthoscellides obtectus when expressed in common beans |
title_full |
Seed storage proteins ARL2 and its variants from the apalocus of wild tepary bean G40199 confers resistance to acanthoscellides obtectus when expressed in common beans |
title_fullStr |
Seed storage proteins ARL2 and its variants from the apalocus of wild tepary bean G40199 confers resistance to acanthoscellides obtectus when expressed in common beans |
title_full_unstemmed |
Seed storage proteins ARL2 and its variants from the apalocus of wild tepary bean G40199 confers resistance to acanthoscellides obtectus when expressed in common beans |
title_sort |
seed storage proteins arl2 and its variants from the apalocus of wild tepary bean g40199 confers resistance to acanthoscellides obtectus when expressed in common beans |
publisher |
African Crop Science Society |
publishDate |
2011 |
url |
https://hdl.handle.net/10568/97068 https://www.ajol.info/index.php/acsj/article/download/74170/64829 |
work_keys_str_mv |
AT kusolwapm seedstorageproteinsarl2anditsvariantsfromtheapalocusofwildteparybeang40199confersresistancetoacanthoscellidesobtectuswhenexpressedincommonbeans AT myersmjr seedstorageproteinsarl2anditsvariantsfromtheapalocusofwildteparybeang40199confersresistancetoacanthoscellidesobtectuswhenexpressedincommonbeans |
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