Embryo-like structures and root regeneration induced by 2, 4-dichlorophenoxyacetic acid in twenty African sweet potato cultivars.
Embryo-like structures were induced from ‘whole leaf’ explants of sixteen of the twenty investigated sweet potato cultivars on Murashige and Skoog (1962) medium supplemented with 2,4 Dichlorophenoxyacetic acid (0.2 mg/L) at the Makerere University Agricultural Research Institute Tissue culture laboratory. Shoot and root regeneration was possible in the non-African cultivar, Jonathan, whereas only root regeneration was successfully induced in five African cultivars. Cultivar type had a highly significant (P < 0.001) effect on frequency of embryo-like structures and efficiency of both shoot and root regeneration. The embryo-like structures induced could be useful for initiation of cell suspensions to enable genetic transformation of African cultivars. The method for de novo regeneration of roots has potential application in the regeneration of plants or hairy root cultures for cultivars that are recalcitrant to shoot regeneration.
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Language: | English |
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Academic Journals
2017
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Subjects: | sweet potatoes, varieties, regeneration, somatic embryogenesis, |
Online Access: | https://hdl.handle.net/10568/92945 https://doi.org/10.5897/AJAR2016.11118 |
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dig-cgspace-10568-929452023-08-03T08:04:09Z Embryo-like structures and root regeneration induced by 2, 4-dichlorophenoxyacetic acid in twenty African sweet potato cultivars. Sefasi, A. Ghislain, M. Kiggundu, R. Ssemakula, G.N. Rukarwa, R. Kreuze, Jan F. Mukasa, S. Mwanga, R.O.M. sweet potatoes varieties regeneration somatic embryogenesis Embryo-like structures were induced from ‘whole leaf’ explants of sixteen of the twenty investigated sweet potato cultivars on Murashige and Skoog (1962) medium supplemented with 2,4 Dichlorophenoxyacetic acid (0.2 mg/L) at the Makerere University Agricultural Research Institute Tissue culture laboratory. Shoot and root regeneration was possible in the non-African cultivar, Jonathan, whereas only root regeneration was successfully induced in five African cultivars. Cultivar type had a highly significant (P < 0.001) effect on frequency of embryo-like structures and efficiency of both shoot and root regeneration. The embryo-like structures induced could be useful for initiation of cell suspensions to enable genetic transformation of African cultivars. The method for de novo regeneration of roots has potential application in the regeneration of plants or hairy root cultures for cultivars that are recalcitrant to shoot regeneration. 2017 2018-05-29T16:20:03Z 2018-05-29T16:20:03Z Journal Article Sefasi, A.; Ghislain, M.; Kiggundu, R.; Ssemakula, G.; Rukarwa, R.; Mwanga, R.; Kreuze, J.; Mukasa, S. 2017. Embryo-like structures and root regeneration induced by 2, 4-dichlorophenoxyacetic acid in twenty African sweet potato cultivars. African Journal of Agricultural Research. ISSN 1991-637X. 12(14):1190-1195. 1991-637X https://hdl.handle.net/10568/92945 https://doi.org/10.5897/AJAR2016.11118 en Open Access 1190-1195 Academic Journals African Journal of Agricultural Research |
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sweet potatoes varieties regeneration somatic embryogenesis sweet potatoes varieties regeneration somatic embryogenesis |
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sweet potatoes varieties regeneration somatic embryogenesis sweet potatoes varieties regeneration somatic embryogenesis Sefasi, A. Ghislain, M. Kiggundu, R. Ssemakula, G.N. Rukarwa, R. Kreuze, Jan F. Mukasa, S. Mwanga, R.O.M. Embryo-like structures and root regeneration induced by 2, 4-dichlorophenoxyacetic acid in twenty African sweet potato cultivars. |
description |
Embryo-like structures were induced from ‘whole leaf’ explants of sixteen of the twenty investigated sweet potato cultivars on Murashige and Skoog (1962) medium supplemented with 2,4 Dichlorophenoxyacetic acid (0.2 mg/L) at the Makerere University Agricultural Research Institute Tissue culture laboratory. Shoot and root regeneration was possible in the non-African cultivar, Jonathan, whereas only root regeneration was successfully induced in five African cultivars. Cultivar type had a highly significant (P < 0.001) effect on frequency of embryo-like structures and efficiency of both shoot and root regeneration. The embryo-like structures induced could be useful for initiation of cell suspensions to enable genetic transformation of African cultivars. The method for de novo regeneration of roots has potential application in the regeneration of plants or hairy root cultures for cultivars that are recalcitrant to shoot regeneration. |
format |
Journal Article |
topic_facet |
sweet potatoes varieties regeneration somatic embryogenesis |
author |
Sefasi, A. Ghislain, M. Kiggundu, R. Ssemakula, G.N. Rukarwa, R. Kreuze, Jan F. Mukasa, S. Mwanga, R.O.M. |
author_facet |
Sefasi, A. Ghislain, M. Kiggundu, R. Ssemakula, G.N. Rukarwa, R. Kreuze, Jan F. Mukasa, S. Mwanga, R.O.M. |
author_sort |
Sefasi, A. |
title |
Embryo-like structures and root regeneration induced by 2, 4-dichlorophenoxyacetic acid in twenty African sweet potato cultivars. |
title_short |
Embryo-like structures and root regeneration induced by 2, 4-dichlorophenoxyacetic acid in twenty African sweet potato cultivars. |
title_full |
Embryo-like structures and root regeneration induced by 2, 4-dichlorophenoxyacetic acid in twenty African sweet potato cultivars. |
title_fullStr |
Embryo-like structures and root regeneration induced by 2, 4-dichlorophenoxyacetic acid in twenty African sweet potato cultivars. |
title_full_unstemmed |
Embryo-like structures and root regeneration induced by 2, 4-dichlorophenoxyacetic acid in twenty African sweet potato cultivars. |
title_sort |
embryo-like structures and root regeneration induced by 2, 4-dichlorophenoxyacetic acid in twenty african sweet potato cultivars. |
publisher |
Academic Journals |
publishDate |
2017 |
url |
https://hdl.handle.net/10568/92945 https://doi.org/10.5897/AJAR2016.11118 |
work_keys_str_mv |
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