Integrative taxonomy of root-knot nematodes reveals multiple independent origins of mitotic parthenogenesis

Integrative taxonomy of root-knot nematodes reveals multiple independent origins of mitotic parthenogenesis

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Main Authors: Janssen, T., Karssen, G., Topalovic, O., Coyne, Danny L., Bert, W.
Format: Journal Article biblioteca
Language:English
Published: Public Library of Science 2017-03-03
Subjects:coffea, meloidogyne, root-knot nematodes, mitotic parthenogenesis, integrative taxonomy,
Online Access:https://hdl.handle.net/10568/80556
https://doi.org/10.1371/journal.pone.0172190
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spelling dig-cgspace-10568-805562023-12-27T19:51:15Z Integrative taxonomy of root-knot nematodes reveals multiple independent origins of mitotic parthenogenesis Janssen, T. Karssen, G. Topalovic, O. Coyne, Danny L. Bert, W. coffea meloidogyne root-knot nematodes mitotic parthenogenesis integrative taxonomy Open Access Journal During sampling of several Coffea arabica plantations in Tanzania severe root galling, caused by a root-knot nematode was observed. From pure cultures, morphology and morphometrics of juveniles and females matched perfectly with Meloidogyne africana, whereas morphology of the males matched identically with those of Meloidogyne decalineata. Based on their Cox1 sequence, however, the recovered juveniles, females and males were confirmed to belong to the same species, creating a taxonomic conundrum. Adding further to this puzzle, re-examination of M. oteifae type material showed insufficient morphological evidence to maintain its status as a separate species. Consequently, M. decalineata and M. oteifae are synonymized with M. africana, which is herewith redescribed based on results of light and scanning electron microscopy, ribosomal and mitochondrial DNA sequences, isozyme electrophoresis, along with bionomic and cytogenetic features. Multi-gene phylogenetic analysis placed M. africana outside of the three major clades, together with M. coffeicola, M. ichinohei and M. camelliae. This phylogenetic position was confirmed by several morphological features, including cellular structure of the spermatheca, egg mass position, perineal pattern and head shape. Moreover, M. africana was found to be a polyphagous species, demonstrating that "early-branching" Meloidogyne spp. are not as oligophagous as had previously been assumed. Cytogenetic information indicates M. africana (2n = 21) and M. ardenensis (2n = 51-54) to be a triploid mitotic parthenogenetic species, revealing at least four independent origins of mitotic parthenogenesis within the genus Meloidogyne. Furthermore, M. mali (n = 12) was found to reproduce by amphimixis, indicating that amphimictic species with a limited number of chromosomes are widespread in the genus, potentially reflecting the ancestral state of the genus. The wide variation in chromosome numbers and associated changes in reproduction modes indicate that cytogenetic evolution played a crucial role in the speciation of root-knot nematodes and plant-parasitic nematodes in general. 2017-03-03 2017-03-29T12:52:34Z 2017-03-29T12:52:34Z Journal Article Janssen, T., Karssen, G., Topalović, O., Coyne, D. & Bert, W. (2017). Integrative taxonomy of root-knot nematodes reveals multiple independent origins of mitotic parthenogenesis. PloS ONE, 12(3), e0172190, 1-31. 1932-6203 https://hdl.handle.net/10568/80556 https://doi.org/10.1371/journal.pone.0172190 en CC-BY-4.0 Open Access 1-31 application/pdf Public Library of Science PLOS ONE
institution CGIAR
collection DSpace
country Francia
countrycode FR
component Bibliográfico
access En linea
databasecode dig-cgspace
tag biblioteca
region Europa del Oeste
libraryname Biblioteca del CGIAR
language English
topic coffea
meloidogyne
root-knot nematodes
mitotic parthenogenesis
integrative taxonomy
coffea
meloidogyne
root-knot nematodes
mitotic parthenogenesis
integrative taxonomy
spellingShingle coffea
meloidogyne
root-knot nematodes
mitotic parthenogenesis
integrative taxonomy
coffea
meloidogyne
root-knot nematodes
mitotic parthenogenesis
integrative taxonomy
Janssen, T.
Karssen, G.
Topalovic, O.
Coyne, Danny L.
Bert, W.
Integrative taxonomy of root-knot nematodes reveals multiple independent origins of mitotic parthenogenesis
description Open Access Journal
format Journal Article
topic_facet coffea
meloidogyne
root-knot nematodes
mitotic parthenogenesis
integrative taxonomy
author Janssen, T.
Karssen, G.
Topalovic, O.
Coyne, Danny L.
Bert, W.
author_facet Janssen, T.
Karssen, G.
Topalovic, O.
Coyne, Danny L.
Bert, W.
author_sort Janssen, T.
title Integrative taxonomy of root-knot nematodes reveals multiple independent origins of mitotic parthenogenesis
title_short Integrative taxonomy of root-knot nematodes reveals multiple independent origins of mitotic parthenogenesis
title_full Integrative taxonomy of root-knot nematodes reveals multiple independent origins of mitotic parthenogenesis
title_fullStr Integrative taxonomy of root-knot nematodes reveals multiple independent origins of mitotic parthenogenesis
title_full_unstemmed Integrative taxonomy of root-knot nematodes reveals multiple independent origins of mitotic parthenogenesis
title_sort integrative taxonomy of root-knot nematodes reveals multiple independent origins of mitotic parthenogenesis
publisher Public Library of Science
publishDate 2017-03-03
url https://hdl.handle.net/10568/80556
https://doi.org/10.1371/journal.pone.0172190
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AT karsseng integrativetaxonomyofrootknotnematodesrevealsmultipleindependentoriginsofmitoticparthenogenesis
AT topalovico integrativetaxonomyofrootknotnematodesrevealsmultipleindependentoriginsofmitoticparthenogenesis
AT coynedannyl integrativetaxonomyofrootknotnematodesrevealsmultipleindependentoriginsofmitoticparthenogenesis
AT bertw integrativetaxonomyofrootknotnematodesrevealsmultipleindependentoriginsofmitoticparthenogenesis
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