Mitochondrial DNA D-loop sequences reveal the genetic diversity of African chicken
Mitochondrial DNA (mtDNA) displacement (D)-loop sequences were used to study the genetic diversity and relationship of African domestic chicken. A total of 398 individuals belonging to 28 populations were sampled from 12 African countries. The hypervariable 1 (HV1) segment of the D-loop was PCR amplified and subsequently sequenced. The sequences of the first 397 nucleotides were used for analysis. Fifty-two haplotypes were identified from 50 polymorphic sites with polymorphism between nucleotides 167 and 397 contributing to 96% of the variation. Phylogenetic analysis indicates that African domestic chicken mtDNA can be grouped into six distinct clades with one to four clades observed in populations. AMOVA analysis indicates that 64.8% of the total sequence variation between haplotypes was present within population and 35.2% between populations. Our results suggest multiple maternal origins for the African domestic chicken.
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TSAP and ILRI
2006
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Subjects: | genetics, poultry, |
Online Access: | https://hdl.handle.net/10568/5522 https://hdl.handle.net/10568/2275 |
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dig-cgspace-10568-55222022-02-18T12:40:11Z Mitochondrial DNA D-loop sequences reveal the genetic diversity of African chicken Mobegi, A.V. genetics poultry Mitochondrial DNA (mtDNA) displacement (D)-loop sequences were used to study the genetic diversity and relationship of African domestic chicken. A total of 398 individuals belonging to 28 populations were sampled from 12 African countries. The hypervariable 1 (HV1) segment of the D-loop was PCR amplified and subsequently sequenced. The sequences of the first 397 nucleotides were used for analysis. Fifty-two haplotypes were identified from 50 polymorphic sites with polymorphism between nucleotides 167 and 397 contributing to 96% of the variation. Phylogenetic analysis indicates that African domestic chicken mtDNA can be grouped into six distinct clades with one to four clades observed in populations. AMOVA analysis indicates that 64.8% of the total sequence variation between haplotypes was present within population and 35.2% between populations. Our results suggest multiple maternal origins for the African domestic chicken. 2006 2011-09-03T18:37:53Z 2011-09-03T18:37:53Z Conference Paper Mobegi, A.V. 2006. Mitochondrial DNA D-loop sequences reveal the genetic diversity of African chicken. In: Rege, J.E.O.; Nyamu, A.M.; Sendalo, D. (eds.). 2006. The role of biotechnology in animal agriculture to address poverty in Africa: Opportunities and challenges. Proceedings of the 4th All Africa Conference on Animal Agriculture and the 31st annual meeting of Tanzania Society for Animal Production, Arusha, Tanzania, 20-24 September 2005. Dar es Salaam, Tanzania: TSAP and Nairobi, Kenya: ILRI. https://hdl.handle.net/10568/5522 https://hdl.handle.net/10568/2275 en Other Open Access TSAP and ILRI |
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genetics poultry genetics poultry Mobegi, A.V. Mitochondrial DNA D-loop sequences reveal the genetic diversity of African chicken |
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Mitochondrial DNA (mtDNA) displacement (D)-loop sequences were used to study the genetic
diversity and relationship of African domestic chicken. A total of 398 individuals belonging to
28 populations were sampled from 12 African countries. The hypervariable 1 (HV1) segment of
the D-loop was PCR amplified and subsequently sequenced. The sequences of the first 397
nucleotides were used for analysis. Fifty-two haplotypes were identified from 50 polymorphic
sites with polymorphism between nucleotides 167 and 397 contributing to 96% of the variation.
Phylogenetic analysis indicates that African domestic chicken mtDNA can be grouped into six
distinct clades with one to four clades observed in populations. AMOVA analysis indicates that
64.8% of the total sequence variation between haplotypes was present within population and
35.2% between populations. Our results suggest multiple maternal origins for the African domestic
chicken. |
format |
Conference Paper |
topic_facet |
genetics poultry |
author |
Mobegi, A.V. |
author_facet |
Mobegi, A.V. |
author_sort |
Mobegi, A.V. |
title |
Mitochondrial DNA D-loop sequences reveal the genetic diversity of African chicken |
title_short |
Mitochondrial DNA D-loop sequences reveal the genetic diversity of African chicken |
title_full |
Mitochondrial DNA D-loop sequences reveal the genetic diversity of African chicken |
title_fullStr |
Mitochondrial DNA D-loop sequences reveal the genetic diversity of African chicken |
title_full_unstemmed |
Mitochondrial DNA D-loop sequences reveal the genetic diversity of African chicken |
title_sort |
mitochondrial dna d-loop sequences reveal the genetic diversity of african chicken |
publisher |
TSAP and ILRI |
publishDate |
2006 |
url |
https://hdl.handle.net/10568/5522 https://hdl.handle.net/10568/2275 |
work_keys_str_mv |
AT mobegiav mitochondrialdnadloopsequencesrevealthegeneticdiversityofafricanchicken |
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1779050565073895424 |