Impact of land use on the distribution and diversity of entomopathogenic nematodes in embu and taita districts, kenya
Natural entomopathogenic nematodes (EPNs) are considered as potential biological control agents against soil-borne insect pests. This study was conducted to determine the impact of land use on the distribution, occurrence and diversity of entomopathogenic nematode community. Isolation of EPNs was done using the baiting technique and application of morphological identification methods revealed presence of the genus Steinernema. Land use intensification negatively affected the occurrence and recovery frequency in soils of Embu and Taita districts. The occurrence of EPNs was high in soils from coffee than maize and beans which had more nematodes than planted forest and napier grass followed by natural forest and tea respectively. PCR-RFLP of the internal transcribed spacer region on the ribosomal(r) DNA of the EPN isolates and digestion of the products by Alu I enzyme showed molecular variations among the isolates. The study has demonstrated that the frequency of occurrence and species variation of EPNs is different in various land uses.
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Format: | Journal Article biblioteca |
Language: | English |
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2011
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Subjects: | entomophilic nematodes, sampling, land use, nemátodos entomófilos, muestreo, utilización de la tierra, |
Online Access: | https://hdl.handle.net/10568/43555 http://www.redalyc.org/articulo.oa?id=93916247008 |
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dig-cgspace-10568-435552023-02-15T05:20:02Z Impact of land use on the distribution and diversity of entomopathogenic nematodes in embu and taita districts, kenya Kawaka, JF Kimenju, J.W. Ayodo, G Mwaniki, SW Muoma, JO Okoth, Sheila A. Orinda, G.O. entomophilic nematodes sampling land use nemátodos entomófilos muestreo utilización de la tierra Natural entomopathogenic nematodes (EPNs) are considered as potential biological control agents against soil-borne insect pests. This study was conducted to determine the impact of land use on the distribution, occurrence and diversity of entomopathogenic nematode community. Isolation of EPNs was done using the baiting technique and application of morphological identification methods revealed presence of the genus Steinernema. Land use intensification negatively affected the occurrence and recovery frequency in soils of Embu and Taita districts. The occurrence of EPNs was high in soils from coffee than maize and beans which had more nematodes than planted forest and napier grass followed by natural forest and tea respectively. PCR-RFLP of the internal transcribed spacer region on the ribosomal(r) DNA of the EPN isolates and digestion of the products by Alu I enzyme showed molecular variations among the isolates. The study has demonstrated that the frequency of occurrence and species variation of EPNs is different in various land uses. 2011 2014-09-24T08:42:19Z 2014-09-24T08:42:19Z Journal Article 1870-0462 https://hdl.handle.net/10568/43555 http://www.redalyc.org/articulo.oa?id=93916247008 en Open Access Tropical and Subtropical Agroecosystems |
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entomophilic nematodes sampling land use nemátodos entomófilos muestreo utilización de la tierra entomophilic nematodes sampling land use nemátodos entomófilos muestreo utilización de la tierra |
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entomophilic nematodes sampling land use nemátodos entomófilos muestreo utilización de la tierra entomophilic nematodes sampling land use nemátodos entomófilos muestreo utilización de la tierra Kawaka, JF Kimenju, J.W. Ayodo, G Mwaniki, SW Muoma, JO Okoth, Sheila A. Orinda, G.O. Impact of land use on the distribution and diversity of entomopathogenic nematodes in embu and taita districts, kenya |
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Natural entomopathogenic nematodes (EPNs) are considered as potential biological control agents against soil-borne insect pests. This study was conducted to determine the impact of land use on the distribution, occurrence and diversity of entomopathogenic nematode community. Isolation of EPNs was done using the baiting technique and application of morphological identification methods revealed presence of the genus Steinernema. Land use intensification negatively affected the occurrence and recovery frequency in soils of Embu and Taita districts. The occurrence of EPNs was high in soils from coffee than maize and beans which had more nematodes than planted forest and napier grass followed by natural forest and tea respectively. PCR-RFLP of the internal transcribed spacer region on the ribosomal(r) DNA of the EPN isolates and digestion of the products by Alu I enzyme showed molecular variations among the isolates. The study has demonstrated that the frequency of occurrence and species variation of EPNs is different in various land uses. |
format |
Journal Article |
topic_facet |
entomophilic nematodes sampling land use nemátodos entomófilos muestreo utilización de la tierra |
author |
Kawaka, JF Kimenju, J.W. Ayodo, G Mwaniki, SW Muoma, JO Okoth, Sheila A. Orinda, G.O. |
author_facet |
Kawaka, JF Kimenju, J.W. Ayodo, G Mwaniki, SW Muoma, JO Okoth, Sheila A. Orinda, G.O. |
author_sort |
Kawaka, JF |
title |
Impact of land use on the distribution and diversity of entomopathogenic nematodes in embu and taita districts, kenya |
title_short |
Impact of land use on the distribution and diversity of entomopathogenic nematodes in embu and taita districts, kenya |
title_full |
Impact of land use on the distribution and diversity of entomopathogenic nematodes in embu and taita districts, kenya |
title_fullStr |
Impact of land use on the distribution and diversity of entomopathogenic nematodes in embu and taita districts, kenya |
title_full_unstemmed |
Impact of land use on the distribution and diversity of entomopathogenic nematodes in embu and taita districts, kenya |
title_sort |
impact of land use on the distribution and diversity of entomopathogenic nematodes in embu and taita districts, kenya |
publishDate |
2011 |
url |
https://hdl.handle.net/10568/43555 http://www.redalyc.org/articulo.oa?id=93916247008 |
work_keys_str_mv |
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