Inheritance and characterization of angular leaf spot resistance gene present in common bean accession G 10474 and identification of an AFLP marker linked to the resistance gene

Angular leaf spot (ALS), caused by the fungus Phaeoisariopsis griseola (Sacc.) Ferr., is one of the most important diseases of common bean (Phaseolus vulgaris L.) in tropical and subtropical bean producing areas. The common bean accession G 10474 has been widely resistant to P. griseola, including pathotype 63-63, one of the most virulent P. griseola pathotypes characterized. The objectives of this study were to determine the inheritance of angular leaf spot resistance and to identify molecular markers linked to the resistance gene in G 10474. Resistance to P. griseola pathotypes 63-63 in G 10474 was investigated in F2 populations derived from a cross between G 10474 and Sprite (universally susceptible). Inoculation of parents, F1, F2, and backcross-derived plants with race 63-63 revealed that a single dominant gene conditioned ALS resistance in G 10474. A combination of the amplified fragment length polymorphism (AFLP) technique and bulked segregant analysis (BSA) was applied to the F2 population to identify molecular markers linked to the ALS resistance gene in G 10474. Three AFLP markers (E-ACA/M-CTT330, E-AAC/M-CAG310, and E-AAC/M-CAT285) segregated in coupling phase with the resistance gene in G 10474. The E-ACA/M-CTT330 marker was successfully converted to a codominant sequence characterized amplified region (SCAR) marker at 5 cM from the resistance gene. Validation of the SCAR marker outside the mapping population showed that the utility of this marker for marker-assisted selection (MAS) was limited to the Andean gene pool of P. vulgaris. Therefore, the SCAR marker we report can only be used to introgress the ALS resistance of G 10474 into Andean backgrounds.

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Main Authors: Mahuku, George S., Montoya, C, Henriquez Naranjo, MA, Jara, Carlos E., Terán Santofimio, Henry, Beebe, Stephen E.
Format: Journal Article biblioteca
Language:English
Published: Wiley 2004-09
Subjects:phaseolus vulgaris, phaeoisariopsis griseola, fungal diseases, genetic inheritance, disease resistance, pathotypes, aflp, enfermedades fungosas, herencia genética, resistencia a la enfermedad, patotipos,
Online Access:https://hdl.handle.net/10568/42979
https://doi.org/10.2135/cropsci2004.1817
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spelling dig-cgspace-10568-429792023-09-10T05:38:58Z Inheritance and characterization of angular leaf spot resistance gene present in common bean accession G 10474 and identification of an AFLP marker linked to the resistance gene Mahuku, George S. Montoya, C Henriquez Naranjo, MA Jara, Carlos E. Terán Santofimio, Henry Beebe, Stephen E. phaseolus vulgaris phaeoisariopsis griseola fungal diseases genetic inheritance disease resistance pathotypes aflp enfermedades fungosas herencia genética resistencia a la enfermedad patotipos Angular leaf spot (ALS), caused by the fungus Phaeoisariopsis griseola (Sacc.) Ferr., is one of the most important diseases of common bean (Phaseolus vulgaris L.) in tropical and subtropical bean producing areas. The common bean accession G 10474 has been widely resistant to P. griseola, including pathotype 63-63, one of the most virulent P. griseola pathotypes characterized. The objectives of this study were to determine the inheritance of angular leaf spot resistance and to identify molecular markers linked to the resistance gene in G 10474. Resistance to P. griseola pathotypes 63-63 in G 10474 was investigated in F2 populations derived from a cross between G 10474 and Sprite (universally susceptible). Inoculation of parents, F1, F2, and backcross-derived plants with race 63-63 revealed that a single dominant gene conditioned ALS resistance in G 10474. A combination of the amplified fragment length polymorphism (AFLP) technique and bulked segregant analysis (BSA) was applied to the F2 population to identify molecular markers linked to the ALS resistance gene in G 10474. Three AFLP markers (E-ACA/M-CTT330, E-AAC/M-CAG310, and E-AAC/M-CAT285) segregated in coupling phase with the resistance gene in G 10474. The E-ACA/M-CTT330 marker was successfully converted to a codominant sequence characterized amplified region (SCAR) marker at 5 cM from the resistance gene. Validation of the SCAR marker outside the mapping population showed that the utility of this marker for marker-assisted selection (MAS) was limited to the Andean gene pool of P. vulgaris. Therefore, the SCAR marker we report can only be used to introgress the ALS resistance of G 10474 into Andean backgrounds. 2004-09 2014-09-24T07:58:52Z 2014-09-24T07:58:52Z Journal Article Mahuku, George S.; Montoya, Carmenza; Henríquez Naranjo, María Antonia; Jara, Carlos E.; Terán Santofimio, Henry; Beebe, Stephen E.. 2004. Inheritance and characterization of angular leaf spot resistance gene present in common bean accession G 10474 and identification of an AFLP marker linked to the resistance gene . Crop Science (USA) 44(5):1817-1824. 1435-0653 https://hdl.handle.net/10568/42979 https://doi.org/10.2135/cropsci2004.1817 en Copyrighted; all rights reserved Limited Access p. 1817-1824 Wiley Crop Science
institution CGIAR
collection DSpace
country Francia
countrycode FR
component Bibliográfico
access En linea
databasecode dig-cgspace
tag biblioteca
region Europa del Oeste
libraryname Biblioteca del CGIAR
language English
topic phaseolus vulgaris
phaeoisariopsis griseola
fungal diseases
genetic inheritance
disease resistance
pathotypes
aflp
enfermedades fungosas
herencia genética
resistencia a la enfermedad
patotipos
phaseolus vulgaris
phaeoisariopsis griseola
fungal diseases
genetic inheritance
disease resistance
pathotypes
aflp
enfermedades fungosas
herencia genética
resistencia a la enfermedad
patotipos
spellingShingle phaseolus vulgaris
phaeoisariopsis griseola
fungal diseases
genetic inheritance
disease resistance
pathotypes
aflp
enfermedades fungosas
herencia genética
resistencia a la enfermedad
patotipos
phaseolus vulgaris
phaeoisariopsis griseola
fungal diseases
genetic inheritance
disease resistance
pathotypes
aflp
enfermedades fungosas
herencia genética
resistencia a la enfermedad
patotipos
Mahuku, George S.
Montoya, C
Henriquez Naranjo, MA
Jara, Carlos E.
Terán Santofimio, Henry
Beebe, Stephen E.
Inheritance and characterization of angular leaf spot resistance gene present in common bean accession G 10474 and identification of an AFLP marker linked to the resistance gene
description Angular leaf spot (ALS), caused by the fungus Phaeoisariopsis griseola (Sacc.) Ferr., is one of the most important diseases of common bean (Phaseolus vulgaris L.) in tropical and subtropical bean producing areas. The common bean accession G 10474 has been widely resistant to P. griseola, including pathotype 63-63, one of the most virulent P. griseola pathotypes characterized. The objectives of this study were to determine the inheritance of angular leaf spot resistance and to identify molecular markers linked to the resistance gene in G 10474. Resistance to P. griseola pathotypes 63-63 in G 10474 was investigated in F2 populations derived from a cross between G 10474 and Sprite (universally susceptible). Inoculation of parents, F1, F2, and backcross-derived plants with race 63-63 revealed that a single dominant gene conditioned ALS resistance in G 10474. A combination of the amplified fragment length polymorphism (AFLP) technique and bulked segregant analysis (BSA) was applied to the F2 population to identify molecular markers linked to the ALS resistance gene in G 10474. Three AFLP markers (E-ACA/M-CTT330, E-AAC/M-CAG310, and E-AAC/M-CAT285) segregated in coupling phase with the resistance gene in G 10474. The E-ACA/M-CTT330 marker was successfully converted to a codominant sequence characterized amplified region (SCAR) marker at 5 cM from the resistance gene. Validation of the SCAR marker outside the mapping population showed that the utility of this marker for marker-assisted selection (MAS) was limited to the Andean gene pool of P. vulgaris. Therefore, the SCAR marker we report can only be used to introgress the ALS resistance of G 10474 into Andean backgrounds.
format Journal Article
topic_facet phaseolus vulgaris
phaeoisariopsis griseola
fungal diseases
genetic inheritance
disease resistance
pathotypes
aflp
enfermedades fungosas
herencia genética
resistencia a la enfermedad
patotipos
author Mahuku, George S.
Montoya, C
Henriquez Naranjo, MA
Jara, Carlos E.
Terán Santofimio, Henry
Beebe, Stephen E.
author_facet Mahuku, George S.
Montoya, C
Henriquez Naranjo, MA
Jara, Carlos E.
Terán Santofimio, Henry
Beebe, Stephen E.
author_sort Mahuku, George S.
title Inheritance and characterization of angular leaf spot resistance gene present in common bean accession G 10474 and identification of an AFLP marker linked to the resistance gene
title_short Inheritance and characterization of angular leaf spot resistance gene present in common bean accession G 10474 and identification of an AFLP marker linked to the resistance gene
title_full Inheritance and characterization of angular leaf spot resistance gene present in common bean accession G 10474 and identification of an AFLP marker linked to the resistance gene
title_fullStr Inheritance and characterization of angular leaf spot resistance gene present in common bean accession G 10474 and identification of an AFLP marker linked to the resistance gene
title_full_unstemmed Inheritance and characterization of angular leaf spot resistance gene present in common bean accession G 10474 and identification of an AFLP marker linked to the resistance gene
title_sort inheritance and characterization of angular leaf spot resistance gene present in common bean accession g 10474 and identification of an aflp marker linked to the resistance gene
publisher Wiley
publishDate 2004-09
url https://hdl.handle.net/10568/42979
https://doi.org/10.2135/cropsci2004.1817
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