Genetic relationships of common bean (Phaseolus vulgaris L.) race chile with wild andean and Mesoamerican germplasm
The Chilean common bean (Phaseolus vulgaris L.) belongs to the cultivated race Chile and its origin is presumably Andean. The objective of this study was to identify the origin of a group of Chilean accessions based on their genetic relationship with wild material from the Mesoamerican and Andean common bean gene pool. To achieve this objective, universal primers of chloroplast DNA (cpDNA) and mitochondrial DNA (mtDNA) were used to detect polymorphism using Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP). Thirty- two genotypes were analyzed, including wild material from Mexico, Ecuador, Peru, Bolivia, and Argentina, as well as Chilean cultivated genotypes belonging to endemic Chilean accession types (To?rtola, Coscorro?n, and Cuyano) and naturalized commercial lines (Frutilla, Bayo, Manteca, and Blanco grande). Results showed a low level of polymorphism for cpDNA (23%) and mtDNA (24%) in wild and cultivated Chilean common bean accessions. Some universal primers and restriction enzyme combinations were more efficient than others in detecting polymorphism. The Chilean materials were closely related to wild accessions collected in Argentina, Bolivia, and Peru indicating their Andean origin. The wild accessions from Ecuador were located in a intermediate position between the Mesoamerican and Andean accessions.
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Format: | Journal Article biblioteca |
Language: | English |
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2011
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Subjects: | common bean, phaseolus vulgaris, chloroplasts, mitochondrial genetics, polymerase chain reaction, dna, cloroplasto, genética mitocondrial, reacción en cadena de la polimerasa, and, |
Online Access: | https://hdl.handle.net/10568/42902 http://www.bioline.org.br/pdf?cj11001 |
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dig-cgspace-10568-429022023-02-15T05:20:31Z Genetic relationships of common bean (Phaseolus vulgaris L.) race chile with wild andean and Mesoamerican germplasm Becerra Velásquez, Viviana L. Paredes-Cárcomo, M Debouck, Daniel G. common bean phaseolus vulgaris chloroplasts mitochondrial genetics polymerase chain reaction dna cloroplasto genética mitocondrial reacción en cadena de la polimerasa and The Chilean common bean (Phaseolus vulgaris L.) belongs to the cultivated race Chile and its origin is presumably Andean. The objective of this study was to identify the origin of a group of Chilean accessions based on their genetic relationship with wild material from the Mesoamerican and Andean common bean gene pool. To achieve this objective, universal primers of chloroplast DNA (cpDNA) and mitochondrial DNA (mtDNA) were used to detect polymorphism using Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP). Thirty- two genotypes were analyzed, including wild material from Mexico, Ecuador, Peru, Bolivia, and Argentina, as well as Chilean cultivated genotypes belonging to endemic Chilean accession types (To?rtola, Coscorro?n, and Cuyano) and naturalized commercial lines (Frutilla, Bayo, Manteca, and Blanco grande). Results showed a low level of polymorphism for cpDNA (23%) and mtDNA (24%) in wild and cultivated Chilean common bean accessions. Some universal primers and restriction enzyme combinations were more efficient than others in detecting polymorphism. The Chilean materials were closely related to wild accessions collected in Argentina, Bolivia, and Peru indicating their Andean origin. The wild accessions from Ecuador were located in a intermediate position between the Mesoamerican and Andean accessions. 2011 2014-09-24T07:58:44Z 2014-09-24T07:58:44Z Journal Article Becerra-Velásquez V., M. Paredes-Cárcomo, D.G. Debouck. 2011. Genetic relationships of common bean (Phaseolus vulgaris L.) race Chile with wild Andean and Mesoamerican germplasm. Chilean Journal Agricultural Research. 71 (1): 3-15 https://hdl.handle.net/10568/42902 http://www.bioline.org.br/pdf?cj11001 en Open Access Chilean Journal of Agricultural Research |
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common bean phaseolus vulgaris chloroplasts mitochondrial genetics polymerase chain reaction dna cloroplasto genética mitocondrial reacción en cadena de la polimerasa and common bean phaseolus vulgaris chloroplasts mitochondrial genetics polymerase chain reaction dna cloroplasto genética mitocondrial reacción en cadena de la polimerasa and |
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common bean phaseolus vulgaris chloroplasts mitochondrial genetics polymerase chain reaction dna cloroplasto genética mitocondrial reacción en cadena de la polimerasa and common bean phaseolus vulgaris chloroplasts mitochondrial genetics polymerase chain reaction dna cloroplasto genética mitocondrial reacción en cadena de la polimerasa and Becerra Velásquez, Viviana L. Paredes-Cárcomo, M Debouck, Daniel G. Genetic relationships of common bean (Phaseolus vulgaris L.) race chile with wild andean and Mesoamerican germplasm |
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The Chilean common bean (Phaseolus vulgaris L.) belongs to the cultivated race Chile and its origin is presumably Andean. The objective of this study was to identify the origin of a group of Chilean accessions based on their genetic relationship with wild material from the Mesoamerican and Andean common bean gene pool. To achieve this objective, universal primers of chloroplast DNA (cpDNA) and mitochondrial DNA (mtDNA) were used to detect polymorphism using Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP). Thirty- two genotypes were analyzed, including wild material from Mexico, Ecuador, Peru, Bolivia, and Argentina, as well as Chilean cultivated genotypes belonging to endemic Chilean accession types (To?rtola, Coscorro?n, and Cuyano) and naturalized commercial lines (Frutilla, Bayo, Manteca, and Blanco grande). Results showed a low level of polymorphism for cpDNA (23%) and mtDNA (24%) in wild and cultivated Chilean common bean accessions. Some universal primers and restriction enzyme combinations were more efficient than others in detecting polymorphism. The Chilean materials were closely related to wild accessions collected in Argentina, Bolivia, and Peru indicating their Andean origin. The wild accessions from Ecuador were located in a intermediate position between the Mesoamerican and Andean accessions. |
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Journal Article |
topic_facet |
common bean phaseolus vulgaris chloroplasts mitochondrial genetics polymerase chain reaction dna cloroplasto genética mitocondrial reacción en cadena de la polimerasa and |
author |
Becerra Velásquez, Viviana L. Paredes-Cárcomo, M Debouck, Daniel G. |
author_facet |
Becerra Velásquez, Viviana L. Paredes-Cárcomo, M Debouck, Daniel G. |
author_sort |
Becerra Velásquez, Viviana L. |
title |
Genetic relationships of common bean (Phaseolus vulgaris L.) race chile with wild andean and Mesoamerican germplasm |
title_short |
Genetic relationships of common bean (Phaseolus vulgaris L.) race chile with wild andean and Mesoamerican germplasm |
title_full |
Genetic relationships of common bean (Phaseolus vulgaris L.) race chile with wild andean and Mesoamerican germplasm |
title_fullStr |
Genetic relationships of common bean (Phaseolus vulgaris L.) race chile with wild andean and Mesoamerican germplasm |
title_full_unstemmed |
Genetic relationships of common bean (Phaseolus vulgaris L.) race chile with wild andean and Mesoamerican germplasm |
title_sort |
genetic relationships of common bean (phaseolus vulgaris l.) race chile with wild andean and mesoamerican germplasm |
publishDate |
2011 |
url |
https://hdl.handle.net/10568/42902 http://www.bioline.org.br/pdf?cj11001 |
work_keys_str_mv |
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_version_ |
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