AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum
Random clones from a cDNA library made from mRNA purified from dissected salivary glands of feeding female Amblyomma variegatum ticks were subjected to single pass sequence analysis. A total of 3,992 sequences with an average read length of 580 nucleotides have been used to construct a gene index called AvGI that consists of 2,109 non-redundant sequences. A provisional gene identity has been assigned to 39% of the database entries by sequence similarity searches against a non-redundant amino acid database and a protein database that has been assigned gene ontology terms. Homologs of genes encoding basic cellular functions including previously characterised enzyme activities, such as stearoyl CoA saturase and protein phosphatase, of ixodid tick salivary glands were found. Several families of abundant cDNA sequences that may code for protein components of tick cement and A. variegatum proteins which may contribute to anti-haemostatic and anti-inflammatory responses, and, one with potential immunosuppressive activity, were also identified. Interference with the function of such proteins might disrupt the life cycle of A. variegatum and help to control this ectoparasite or to reduce its ability to transmit disease causing organisms. AvGI represents an electronic knowledge base, which can be used to launch investigations of the biology of the salivary glands of this tick species.
Main Authors: | Nene, Vishvanath, Lee, D., Quackenbush, J., Skilton, Robert A., Mwaura, S., Gardner, M.J., Bishop, Richard P. |
---|---|
Format: | Journal Article biblioteca |
Language: | English |
Published: |
Elsevier
2002-11
|
Subjects: | amblyomma variegatum, genes, salivary glands, metastigmata, dna, |
Online Access: | https://hdl.handle.net/10568/32955 https://doi.org/10.1016/S0020-7519(02)00159-5 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Identification of candidate sialome components expressed in ixodid tick salivary glands using secretion signal complementation in mammalian cells
by: Lambson, B., et al.
Published: (2005-08) -
Genes transcribed in the salivary glands of female Rhipicephalus appendiculatus ticks infected with Theileria parva
by: Nene, Vishvanath, et al.
Published: (2004-10) -
Detection of Theileria parva in the salivary glands of infected Rhipicephalus appendiculatus ticks with DNA probes
by: Conrad, P.A., et al.
Published: (1989) -
Characterization of tick antigens inducing host immune resistance. 1. Immunization of guinea pigs with Amblyomma americanum-derived salivary gland extracts and identification of an important salivary gland protein antigen with guinea pig anti-tick antibodies
by: Brown, S.J., et al.
Published: (1984) -
Biology and ecology of Ablyomma variegatum in Guadeloupe and its importance in eradication strategy
by: Barre, N., et al.
Published: (1989)