AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum
Random clones from a cDNA library made from mRNA purified from dissected salivary glands of feeding female Amblyomma variegatum ticks were subjected to single pass sequence analysis. A total of 3,992 sequences with an average read length of 580 nucleotides have been used to construct a gene index called AvGI that consists of 2,109 non-redundant sequences. A provisional gene identity has been assigned to 39% of the database entries by sequence similarity searches against a non-redundant amino acid database and a protein database that has been assigned gene ontology terms. Homologs of genes encoding basic cellular functions including previously characterised enzyme activities, such as stearoyl CoA saturase and protein phosphatase, of ixodid tick salivary glands were found. Several families of abundant cDNA sequences that may code for protein components of tick cement and A. variegatum proteins which may contribute to anti-haemostatic and anti-inflammatory responses, and, one with potential immunosuppressive activity, were also identified. Interference with the function of such proteins might disrupt the life cycle of A. variegatum and help to control this ectoparasite or to reduce its ability to transmit disease causing organisms. AvGI represents an electronic knowledge base, which can be used to launch investigations of the biology of the salivary glands of this tick species.
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Format: | Journal Article biblioteca |
Language: | English |
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Elsevier
2002-11
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Subjects: | amblyomma variegatum, genes, salivary glands, metastigmata, dna, |
Online Access: | https://hdl.handle.net/10568/32955 https://doi.org/10.1016/S0020-7519(02)00159-5 |
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dig-cgspace-10568-329552023-12-08T19:36:04Z AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum Nene, Vishvanath Lee, D. Quackenbush, J. Skilton, Robert A. Mwaura, S. Gardner, M.J. Bishop, Richard P. amblyomma variegatum genes salivary glands metastigmata dna Random clones from a cDNA library made from mRNA purified from dissected salivary glands of feeding female Amblyomma variegatum ticks were subjected to single pass sequence analysis. A total of 3,992 sequences with an average read length of 580 nucleotides have been used to construct a gene index called AvGI that consists of 2,109 non-redundant sequences. A provisional gene identity has been assigned to 39% of the database entries by sequence similarity searches against a non-redundant amino acid database and a protein database that has been assigned gene ontology terms. Homologs of genes encoding basic cellular functions including previously characterised enzyme activities, such as stearoyl CoA saturase and protein phosphatase, of ixodid tick salivary glands were found. Several families of abundant cDNA sequences that may code for protein components of tick cement and A. variegatum proteins which may contribute to anti-haemostatic and anti-inflammatory responses, and, one with potential immunosuppressive activity, were also identified. Interference with the function of such proteins might disrupt the life cycle of A. variegatum and help to control this ectoparasite or to reduce its ability to transmit disease causing organisms. AvGI represents an electronic knowledge base, which can be used to launch investigations of the biology of the salivary glands of this tick species. 2002-11 2013-07-03T05:25:49Z 2013-07-03T05:25:49Z Journal Article International Journal for Parasitology;32(12): 1447-1456 0020-7519 https://hdl.handle.net/10568/32955 https://doi.org/10.1016/S0020-7519(02)00159-5 en Copyrighted; all rights reserved Limited Access p. 1447-1456 Elsevier International Journal for Parasitology |
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amblyomma variegatum genes salivary glands metastigmata dna amblyomma variegatum genes salivary glands metastigmata dna |
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amblyomma variegatum genes salivary glands metastigmata dna amblyomma variegatum genes salivary glands metastigmata dna Nene, Vishvanath Lee, D. Quackenbush, J. Skilton, Robert A. Mwaura, S. Gardner, M.J. Bishop, Richard P. AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum |
description |
Random clones from a cDNA library made from mRNA purified from dissected salivary glands of feeding female Amblyomma variegatum ticks were subjected to single pass sequence analysis. A total of 3,992 sequences with an average read length of 580 nucleotides have been used to construct a gene index called AvGI that consists of 2,109 non-redundant sequences. A provisional gene identity has been assigned to 39% of the database entries by sequence similarity searches against a non-redundant amino acid database and a protein database that has been assigned gene ontology terms. Homologs of genes encoding basic cellular functions including previously characterised enzyme activities, such as stearoyl CoA saturase and protein phosphatase, of ixodid tick salivary glands were found. Several families of abundant cDNA sequences that may code for protein components of tick cement and A. variegatum proteins which may contribute to anti-haemostatic and anti-inflammatory responses, and, one with potential immunosuppressive activity, were also identified. Interference with the function of such proteins might disrupt the life cycle of A. variegatum and help to control this ectoparasite or to reduce its ability to transmit disease causing organisms. AvGI represents an electronic knowledge base, which can be used to launch investigations of the biology of the salivary glands of this tick species. |
format |
Journal Article |
topic_facet |
amblyomma variegatum genes salivary glands metastigmata dna |
author |
Nene, Vishvanath Lee, D. Quackenbush, J. Skilton, Robert A. Mwaura, S. Gardner, M.J. Bishop, Richard P. |
author_facet |
Nene, Vishvanath Lee, D. Quackenbush, J. Skilton, Robert A. Mwaura, S. Gardner, M.J. Bishop, Richard P. |
author_sort |
Nene, Vishvanath |
title |
AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum |
title_short |
AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum |
title_full |
AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum |
title_fullStr |
AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum |
title_full_unstemmed |
AvGI, an index of genes transcribed in the salivary glands of the ixodid tick Amblyomma variegatum |
title_sort |
avgi, an index of genes transcribed in the salivary glands of the ixodid tick amblyomma variegatum |
publisher |
Elsevier |
publishDate |
2002-11 |
url |
https://hdl.handle.net/10568/32955 https://doi.org/10.1016/S0020-7519(02)00159-5 |
work_keys_str_mv |
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