Three genes and two isozymes: Gene conversion and the compartmentalization and expression of the phosphoglycerate kinases of Trypanosoma (Nannomonas) congolense
The glycosome, a microbody organelle found only in kinetoplastid protozoa, compartmentalizes the first six enzymes of glycolysis. In order to better understand the regulation and targeting of glycolytic enzymes in trypanosomes. We have cloned and analyzed the three genes within the complex is similar to that of Trypanosoma brucei brucei. The nucleotide and amino-acid sequences, including those of the novel high-molecular-weight 56PGK, show substantial cross-species similarity. However, the two downstream genes, clPGK and c2PGK, encode identical isozymes in T. congolense, while they encode distinct glycosomal and cytoplasmic isozymes in T. brucei. Western analysis also indicated that there are only two isozymes in T. congolense and that these are constitutively expressed. Differential digitonin solubilization of the trypanosomes indicated that 56PGK is primarily localized to the glycosome, as expected, and that cl/c2PGK is cytoplasmic, Northern analysis demonstrates that while 56PGK is constitutively expressed, clPGK and c2PGK mRNAs are differentially expressed in the T. congolense developmental stages. This work demonstrates that T. congolense has only one PGK isozyme, 56PGK, that is predominantly localized in glycosomes.
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Format: | Journal Article biblioteca |
Language: | English |
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Elsevier
1995-02
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Subjects: | trypanosomes (phytomonas), trypanosoma congolense, gene expression, glycolysis, transferases, molecular biology, parasitology, |
Online Access: | https://hdl.handle.net/10568/29267 https://doi.org/10.1016/0166-6851(94)00208-5 |
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dig-cgspace-10568-292672023-12-08T19:36:04Z Three genes and two isozymes: Gene conversion and the compartmentalization and expression of the phosphoglycerate kinases of Trypanosoma (Nannomonas) congolense Parker, H.L. Hill, T. Alexander, K. Murphy, N.B. Fish, W.R. Parsons, M. trypanosomes (phytomonas) trypanosoma congolense gene expression glycolysis transferases molecular biology parasitology The glycosome, a microbody organelle found only in kinetoplastid protozoa, compartmentalizes the first six enzymes of glycolysis. In order to better understand the regulation and targeting of glycolytic enzymes in trypanosomes. We have cloned and analyzed the three genes within the complex is similar to that of Trypanosoma brucei brucei. The nucleotide and amino-acid sequences, including those of the novel high-molecular-weight 56PGK, show substantial cross-species similarity. However, the two downstream genes, clPGK and c2PGK, encode identical isozymes in T. congolense, while they encode distinct glycosomal and cytoplasmic isozymes in T. brucei. Western analysis also indicated that there are only two isozymes in T. congolense and that these are constitutively expressed. Differential digitonin solubilization of the trypanosomes indicated that 56PGK is primarily localized to the glycosome, as expected, and that cl/c2PGK is cytoplasmic, Northern analysis demonstrates that while 56PGK is constitutively expressed, clPGK and c2PGK mRNAs are differentially expressed in the T. congolense developmental stages. This work demonstrates that T. congolense has only one PGK isozyme, 56PGK, that is predominantly localized in glycosomes. 1995-02 2013-06-11T09:22:59Z 2013-06-11T09:22:59Z Journal Article Molecular and Biochemical Parasitology;69: 269-279 0166-6851 https://hdl.handle.net/10568/29267 https://doi.org/10.1016/0166-6851(94)00208-5 en Copyrighted; all rights reserved Limited Access p. 269-279 Elsevier Molecular and Biochemical Parasitology |
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trypanosomes (phytomonas) trypanosoma congolense gene expression glycolysis transferases molecular biology parasitology trypanosomes (phytomonas) trypanosoma congolense gene expression glycolysis transferases molecular biology parasitology |
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trypanosomes (phytomonas) trypanosoma congolense gene expression glycolysis transferases molecular biology parasitology trypanosomes (phytomonas) trypanosoma congolense gene expression glycolysis transferases molecular biology parasitology Parker, H.L. Hill, T. Alexander, K. Murphy, N.B. Fish, W.R. Parsons, M. Three genes and two isozymes: Gene conversion and the compartmentalization and expression of the phosphoglycerate kinases of Trypanosoma (Nannomonas) congolense |
description |
The glycosome, a microbody organelle found only in kinetoplastid protozoa, compartmentalizes the first six enzymes of glycolysis. In order to better understand the regulation and targeting of glycolytic enzymes in trypanosomes. We have cloned and analyzed the three genes within the complex is similar to that of Trypanosoma brucei brucei. The nucleotide and amino-acid sequences, including those of the novel high-molecular-weight 56PGK, show substantial cross-species similarity. However, the two downstream genes, clPGK and c2PGK, encode identical isozymes in T. congolense, while they encode distinct glycosomal and cytoplasmic isozymes in T. brucei. Western analysis also indicated that there are only two isozymes in T. congolense and that these are constitutively expressed. Differential digitonin solubilization of the trypanosomes indicated that 56PGK is primarily localized to the glycosome, as expected, and that cl/c2PGK is cytoplasmic, Northern analysis demonstrates that while 56PGK is constitutively expressed, clPGK and c2PGK mRNAs are differentially expressed in the T. congolense developmental stages. This work demonstrates that T. congolense has only one PGK isozyme, 56PGK, that is predominantly localized in glycosomes. |
format |
Journal Article |
topic_facet |
trypanosomes (phytomonas) trypanosoma congolense gene expression glycolysis transferases molecular biology parasitology |
author |
Parker, H.L. Hill, T. Alexander, K. Murphy, N.B. Fish, W.R. Parsons, M. |
author_facet |
Parker, H.L. Hill, T. Alexander, K. Murphy, N.B. Fish, W.R. Parsons, M. |
author_sort |
Parker, H.L. |
title |
Three genes and two isozymes: Gene conversion and the compartmentalization and expression of the phosphoglycerate kinases of Trypanosoma (Nannomonas) congolense |
title_short |
Three genes and two isozymes: Gene conversion and the compartmentalization and expression of the phosphoglycerate kinases of Trypanosoma (Nannomonas) congolense |
title_full |
Three genes and two isozymes: Gene conversion and the compartmentalization and expression of the phosphoglycerate kinases of Trypanosoma (Nannomonas) congolense |
title_fullStr |
Three genes and two isozymes: Gene conversion and the compartmentalization and expression of the phosphoglycerate kinases of Trypanosoma (Nannomonas) congolense |
title_full_unstemmed |
Three genes and two isozymes: Gene conversion and the compartmentalization and expression of the phosphoglycerate kinases of Trypanosoma (Nannomonas) congolense |
title_sort |
three genes and two isozymes: gene conversion and the compartmentalization and expression of the phosphoglycerate kinases of trypanosoma (nannomonas) congolense |
publisher |
Elsevier |
publishDate |
1995-02 |
url |
https://hdl.handle.net/10568/29267 https://doi.org/10.1016/0166-6851(94)00208-5 |
work_keys_str_mv |
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