Evaluation of the recognition of Theileria parva vaccine candidate antigens by cytotoxic T lymphocytes from Zebu cattle
East Coast fever (ECF) is a highly fatal lymphoproliferative disease of cattle caused by Theileria parva, a tick-borne intracellular apicomplexan parasite. Parasite antigens that are targets of protective cytotoxic T lymphocyte (CTL) responses are required to formulate a sub-unit vaccine against ECF. A number of CTL target antigens have recently been identified and initial evaluation has shown their vaccine potential. This study aimed to evaluate whether these antigens were recognised by CTL obtained from six genetically diverse Zebu cattle immunized with a cocktail of T. parva stocks. T. parva Muguga specific polyclonal CD8(+) CTL lines were generated and confirmed to specifically lyse autologous infected cells. CTL recognition of autologous skin fibroblasts (iSF) transduced with recombinant modified vaccinia virus Ankara strain (MVA) expressing previously identified T. parva Muguga vaccine candidate antigens was evaluated using an IFN-gamma ELISpot assay. CTL lines from one of the four calves, BY120, responded specifically to cells infected with MVA expressing the antigen Tp2 and synthetic peptides were employed to map a new CTL epitope on this antigen. Immunoscreening of the T. parva genome with these CTL lines should identify novel antigens that will constitute valuable additions to the vaccine candidates currently being evaluated.
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Format: | Journal Article biblioteca |
Language: | English |
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Elsevier
2008-02
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Subjects: | vaccines, theileria parva, |
Online Access: | https://hdl.handle.net/10568/1942 https://doi.org/10.1016/j.vetimm.2007.09.012 |
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dig-cgspace-10568-19422023-12-08T19:36:04Z Evaluation of the recognition of Theileria parva vaccine candidate antigens by cytotoxic T lymphocytes from Zebu cattle Akoolo, L. Pelle, Roger Saya, R. Awino, Elias Nyanjui, J. Taracha, E.L.N. Kanyari, P. Mwangi, D.M. Graham, S.P. vaccines theileria parva East Coast fever (ECF) is a highly fatal lymphoproliferative disease of cattle caused by Theileria parva, a tick-borne intracellular apicomplexan parasite. Parasite antigens that are targets of protective cytotoxic T lymphocyte (CTL) responses are required to formulate a sub-unit vaccine against ECF. A number of CTL target antigens have recently been identified and initial evaluation has shown their vaccine potential. This study aimed to evaluate whether these antigens were recognised by CTL obtained from six genetically diverse Zebu cattle immunized with a cocktail of T. parva stocks. T. parva Muguga specific polyclonal CD8(+) CTL lines were generated and confirmed to specifically lyse autologous infected cells. CTL recognition of autologous skin fibroblasts (iSF) transduced with recombinant modified vaccinia virus Ankara strain (MVA) expressing previously identified T. parva Muguga vaccine candidate antigens was evaluated using an IFN-gamma ELISpot assay. CTL lines from one of the four calves, BY120, responded specifically to cells infected with MVA expressing the antigen Tp2 and synthetic peptides were employed to map a new CTL epitope on this antigen. Immunoscreening of the T. parva genome with these CTL lines should identify novel antigens that will constitute valuable additions to the vaccine candidates currently being evaluated. 2008-02 2010-06-14T13:01:22Z 2010-06-14T13:01:22Z Journal Article Akoolo, L.; Pellé. R.; Saya, R.; Awino, E.; Nyanjui, J.; Taracha, E.L.; Kanyari, P.; Mwangi, D.M.; Graham, S.P. 2008. Evaluation of the recognition of Theileria parva vaccine candidate antigens by cytotoxic T lymphocytes from Zebu cattle. Veterinary Immunology and Immunopathology 121(3-4):216-221. 0165-2427 https://hdl.handle.net/10568/1942 https://doi.org/10.1016/j.vetimm.2007.09.012 en Copyrighted; all rights reserved Limited Access p. 216-221 Elsevier Veterinary Immunology and Immunopathology |
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vaccines theileria parva vaccines theileria parva Akoolo, L. Pelle, Roger Saya, R. Awino, Elias Nyanjui, J. Taracha, E.L.N. Kanyari, P. Mwangi, D.M. Graham, S.P. Evaluation of the recognition of Theileria parva vaccine candidate antigens by cytotoxic T lymphocytes from Zebu cattle |
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East Coast fever (ECF) is a highly fatal lymphoproliferative disease of cattle caused by Theileria parva, a tick-borne intracellular apicomplexan parasite. Parasite antigens that are targets of protective cytotoxic T lymphocyte (CTL) responses are required to formulate a sub-unit vaccine against ECF. A number of CTL target antigens have recently been identified and initial evaluation has shown their vaccine potential. This study aimed to evaluate whether these antigens were recognised by CTL obtained from six genetically diverse Zebu cattle immunized with a cocktail of T. parva stocks. T. parva Muguga specific polyclonal CD8(+) CTL lines were generated and confirmed to specifically lyse autologous infected cells. CTL recognition of autologous skin fibroblasts (iSF) transduced with recombinant modified vaccinia virus Ankara strain (MVA) expressing previously identified T. parva Muguga vaccine candidate antigens was evaluated using an IFN-gamma ELISpot assay. CTL lines from one of the four calves, BY120, responded specifically to cells infected with MVA expressing the antigen Tp2 and synthetic peptides were employed to map a new CTL epitope on this antigen. Immunoscreening of the T. parva genome with these CTL lines should identify novel antigens that will constitute valuable additions to the vaccine candidates currently being evaluated. |
format |
Journal Article |
topic_facet |
vaccines theileria parva |
author |
Akoolo, L. Pelle, Roger Saya, R. Awino, Elias Nyanjui, J. Taracha, E.L.N. Kanyari, P. Mwangi, D.M. Graham, S.P. |
author_facet |
Akoolo, L. Pelle, Roger Saya, R. Awino, Elias Nyanjui, J. Taracha, E.L.N. Kanyari, P. Mwangi, D.M. Graham, S.P. |
author_sort |
Akoolo, L. |
title |
Evaluation of the recognition of Theileria parva vaccine candidate antigens by cytotoxic T lymphocytes from Zebu cattle |
title_short |
Evaluation of the recognition of Theileria parva vaccine candidate antigens by cytotoxic T lymphocytes from Zebu cattle |
title_full |
Evaluation of the recognition of Theileria parva vaccine candidate antigens by cytotoxic T lymphocytes from Zebu cattle |
title_fullStr |
Evaluation of the recognition of Theileria parva vaccine candidate antigens by cytotoxic T lymphocytes from Zebu cattle |
title_full_unstemmed |
Evaluation of the recognition of Theileria parva vaccine candidate antigens by cytotoxic T lymphocytes from Zebu cattle |
title_sort |
evaluation of the recognition of theileria parva vaccine candidate antigens by cytotoxic t lymphocytes from zebu cattle |
publisher |
Elsevier |
publishDate |
2008-02 |
url |
https://hdl.handle.net/10568/1942 https://doi.org/10.1016/j.vetimm.2007.09.012 |
work_keys_str_mv |
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