Novel Genomic Regions Linked to Ascochyta Blight Resistance in Two Differentially Resistant Cultivars of Chickpea
Ascochyta blight (AB), caused by the fungal pathogen Ascochyta rabiei, is a devastating foliar disease of chickpea (Cicer arietinum L.). The genotyping-by-sequencing (GBS)-based approach was deployed for mapping QTLs associated with AB resistance in chickpea in two recombinant inbred line populations derived from two crosses (AB3279 derived from ILC 1929 × ILC 3279 and AB482 derived from ILC 1929 × ILC 482) and tested in six different environments. Twenty-one different genomic regions linked to AB resistance were identified in regions CalG02 and CalG04 in both populations AB3279 and AB482. These regions contain 1,118 SNPs significantly associated with AB resistance (p ≤ 0.001), which explained 11.2–39.3% of the phenotypic variation (PVE). Nine of the AB resistance-associated genomic regions were newly detected in this study, while twelve regions were known from previous AB studies. The proposed physical map narrows down AB resistance to consistent genomic regions identified across different environments. Gene ontology (GO) assigned these QTLs to 319 genes, many of which were associated with stress and disease resistance, and with most important genes belonging to resistance gene families such as leucine-rich repeat (LRR) and transcription factor families. Our results indicate that the flowering-associated gene GIGANTEA is a possible key factor in AB resistance in chickpea. The results have identified AB resistance-associated regions on the physical genetic map of chickpea and allowed for the identification of associated markers that will help in breeding of AB-resistant varieties.
Main Authors: | , , , , , , , , , , , , |
---|---|
Format: | Journal Article biblioteca |
Language: | English |
Published: |
Frontiers Media
2022-04-25
|
Subjects: | goal 2 zero hunger, Chickpea, ascochyta blight, resistance, quantitative trait loci (qtls), chickpea, nutrition, health and food security, genotyping-by-sequencing (gbs), |
Online Access: | https://hdl.handle.net/10568/125765 https://doi.org/10.5281/zenodo.5236818 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cgspace-10568-125765 |
---|---|
record_format |
koha |
spelling |
dig-cgspace-10568-1257652023-10-17T17:12:45Z Novel Genomic Regions Linked to Ascochyta Blight Resistance in Two Differentially Resistant Cultivars of Chickpea Alo, Fida Ran, Anupalli Baum, Michael Singh, Sarvjeet Kehel, Zakaria Rani, Upasana Udupa, Sripada M. El-Shamaa, Khaled Alsamman, Alsamman Istanbuli, Tawffiq Attar, Basem Hamwieh, Aladdin Amri, Ahmed goal 2 zero hunger Chickpea ascochyta blight resistance quantitative trait loci (qtls) chickpea nutrition, health and food security genotyping-by-sequencing (gbs) Ascochyta blight (AB), caused by the fungal pathogen Ascochyta rabiei, is a devastating foliar disease of chickpea (Cicer arietinum L.). The genotyping-by-sequencing (GBS)-based approach was deployed for mapping QTLs associated with AB resistance in chickpea in two recombinant inbred line populations derived from two crosses (AB3279 derived from ILC 1929 × ILC 3279 and AB482 derived from ILC 1929 × ILC 482) and tested in six different environments. Twenty-one different genomic regions linked to AB resistance were identified in regions CalG02 and CalG04 in both populations AB3279 and AB482. These regions contain 1,118 SNPs significantly associated with AB resistance (p ≤ 0.001), which explained 11.2–39.3% of the phenotypic variation (PVE). Nine of the AB resistance-associated genomic regions were newly detected in this study, while twelve regions were known from previous AB studies. The proposed physical map narrows down AB resistance to consistent genomic regions identified across different environments. Gene ontology (GO) assigned these QTLs to 319 genes, many of which were associated with stress and disease resistance, and with most important genes belonging to resistance gene families such as leucine-rich repeat (LRR) and transcription factor families. Our results indicate that the flowering-associated gene GIGANTEA is a possible key factor in AB resistance in chickpea. The results have identified AB resistance-associated regions on the physical genetic map of chickpea and allowed for the identification of associated markers that will help in breeding of AB-resistant varieties. 2022-04-25 2022-12-02T16:59:18Z 2022-12-02T16:59:18Z Journal Article Fida Alo, Anupalli Ran, Michael Baum, Sarvjeet Singh, Zakaria Kehel, Upasana Rani, Sripada M. Udupa, Khaled El-Shamaa, Alsamman Alsamman, Tawffiq Istanbuli, Basem Attar, Aladdin Hamwieh, Ahmed Amri. (25/4/2022). Novel Genomic Regions Linked to Ascochyta Blight Resistance in Two Differentially Resistant Cultivars of Chickpea. Frontiers in Plant Science, 13. 1664-462X https://hdl.handle.net/10568/125765 https://doi.org/10.5281/zenodo.5236818 en CC-BY-4.0 Open Access application/pdf Frontiers Media Frontiers in Plant Science |
institution |
CGIAR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cgspace |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CGIAR |
language |
English |
topic |
goal 2 zero hunger Chickpea ascochyta blight resistance quantitative trait loci (qtls) chickpea nutrition, health and food security genotyping-by-sequencing (gbs) goal 2 zero hunger Chickpea ascochyta blight resistance quantitative trait loci (qtls) chickpea nutrition, health and food security genotyping-by-sequencing (gbs) |
spellingShingle |
goal 2 zero hunger Chickpea ascochyta blight resistance quantitative trait loci (qtls) chickpea nutrition, health and food security genotyping-by-sequencing (gbs) goal 2 zero hunger Chickpea ascochyta blight resistance quantitative trait loci (qtls) chickpea nutrition, health and food security genotyping-by-sequencing (gbs) Alo, Fida Ran, Anupalli Baum, Michael Singh, Sarvjeet Kehel, Zakaria Rani, Upasana Udupa, Sripada M. El-Shamaa, Khaled Alsamman, Alsamman Istanbuli, Tawffiq Attar, Basem Hamwieh, Aladdin Amri, Ahmed Novel Genomic Regions Linked to Ascochyta Blight Resistance in Two Differentially Resistant Cultivars of Chickpea |
description |
Ascochyta blight (AB), caused by the fungal pathogen Ascochyta rabiei, is a devastating foliar disease of chickpea (Cicer arietinum L.). The genotyping-by-sequencing (GBS)-based approach was deployed for mapping QTLs associated with AB resistance in chickpea in two recombinant inbred line populations derived from two crosses (AB3279 derived from ILC 1929 × ILC 3279 and AB482 derived from ILC 1929 × ILC 482) and tested in six different environments. Twenty-one different genomic regions linked to AB resistance were identified in regions CalG02 and CalG04 in both populations AB3279 and AB482. These regions contain 1,118 SNPs significantly associated with AB resistance (p ≤ 0.001), which explained 11.2–39.3% of the phenotypic variation (PVE). Nine of the AB resistance-associated genomic regions were newly detected in this study, while twelve regions were known from previous AB studies. The proposed physical map narrows down AB resistance to consistent genomic regions identified across different environments. Gene ontology (GO) assigned these QTLs to 319 genes, many of which were associated with stress and disease resistance, and with most important genes belonging to resistance gene families such as leucine-rich repeat (LRR) and transcription factor families. Our results indicate that the flowering-associated gene GIGANTEA is a possible key factor in AB resistance in chickpea. The results have identified AB resistance-associated regions on the physical genetic map of chickpea and allowed for the identification of associated markers that will help in breeding of AB-resistant varieties. |
format |
Journal Article |
topic_facet |
goal 2 zero hunger Chickpea ascochyta blight resistance quantitative trait loci (qtls) chickpea nutrition, health and food security genotyping-by-sequencing (gbs) |
author |
Alo, Fida Ran, Anupalli Baum, Michael Singh, Sarvjeet Kehel, Zakaria Rani, Upasana Udupa, Sripada M. El-Shamaa, Khaled Alsamman, Alsamman Istanbuli, Tawffiq Attar, Basem Hamwieh, Aladdin Amri, Ahmed |
author_facet |
Alo, Fida Ran, Anupalli Baum, Michael Singh, Sarvjeet Kehel, Zakaria Rani, Upasana Udupa, Sripada M. El-Shamaa, Khaled Alsamman, Alsamman Istanbuli, Tawffiq Attar, Basem Hamwieh, Aladdin Amri, Ahmed |
author_sort |
Alo, Fida |
title |
Novel Genomic Regions Linked to Ascochyta Blight Resistance in Two Differentially Resistant Cultivars of Chickpea |
title_short |
Novel Genomic Regions Linked to Ascochyta Blight Resistance in Two Differentially Resistant Cultivars of Chickpea |
title_full |
Novel Genomic Regions Linked to Ascochyta Blight Resistance in Two Differentially Resistant Cultivars of Chickpea |
title_fullStr |
Novel Genomic Regions Linked to Ascochyta Blight Resistance in Two Differentially Resistant Cultivars of Chickpea |
title_full_unstemmed |
Novel Genomic Regions Linked to Ascochyta Blight Resistance in Two Differentially Resistant Cultivars of Chickpea |
title_sort |
novel genomic regions linked to ascochyta blight resistance in two differentially resistant cultivars of chickpea |
publisher |
Frontiers Media |
publishDate |
2022-04-25 |
url |
https://hdl.handle.net/10568/125765 https://doi.org/10.5281/zenodo.5236818 |
work_keys_str_mv |
AT alofida novelgenomicregionslinkedtoascochytablightresistanceintwodifferentiallyresistantcultivarsofchickpea AT rananupalli novelgenomicregionslinkedtoascochytablightresistanceintwodifferentiallyresistantcultivarsofchickpea AT baummichael novelgenomicregionslinkedtoascochytablightresistanceintwodifferentiallyresistantcultivarsofchickpea AT singhsarvjeet novelgenomicregionslinkedtoascochytablightresistanceintwodifferentiallyresistantcultivarsofchickpea AT kehelzakaria novelgenomicregionslinkedtoascochytablightresistanceintwodifferentiallyresistantcultivarsofchickpea AT raniupasana novelgenomicregionslinkedtoascochytablightresistanceintwodifferentiallyresistantcultivarsofchickpea AT udupasripadam novelgenomicregionslinkedtoascochytablightresistanceintwodifferentiallyresistantcultivarsofchickpea AT elshamaakhaled novelgenomicregionslinkedtoascochytablightresistanceintwodifferentiallyresistantcultivarsofchickpea AT alsammanalsamman novelgenomicregionslinkedtoascochytablightresistanceintwodifferentiallyresistantcultivarsofchickpea AT istanbulitawffiq novelgenomicregionslinkedtoascochytablightresistanceintwodifferentiallyresistantcultivarsofchickpea AT attarbasem novelgenomicregionslinkedtoascochytablightresistanceintwodifferentiallyresistantcultivarsofchickpea AT hamwiehaladdin novelgenomicregionslinkedtoascochytablightresistanceintwodifferentiallyresistantcultivarsofchickpea AT amriahmed novelgenomicregionslinkedtoascochytablightresistanceintwodifferentiallyresistantcultivarsofchickpea |
_version_ |
1781882483595280384 |