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A transcriptomic analysis of Stylo [Stylosanthes guianensis (Aubl.) Sw.] provides novel insights into the basis of salinity tolerance
Tropical areas have a large distribution of saline soils and tidal flats with a high salinity level. Salinity stress is a key factor limiting the widespread use of tropical forage such as Stylosanthes guianensis (Aubl.) Sw. This study was designed to screen the salinity tolerance of 84 S. guianensis accessions; In a greenhouse experiment, plants were subjected to Hoagland solution or Hoagland solution with 200 mM NaCl for up to 15 days. Salinity tolerant accession CIAT11365 and salinity sensitive accession FM05-2 were obtained based on withered leaf rate (WLR). Further verification of salinity tolerance in CIAT11365 and FM05-2 with different salinity gradients showed that salinity stress increased WLR and decreased relative chlorophyll content (SPAD), maximum photochemical efficiency of photosystem II (Fv/Fm), and photosynthetic rate (Pn) in FM05-2, but CIAT11365 exhibited lower WLR and higher SPAD, Fv/Fm, and Pn. Leaf RNA-Seq revealed that Ca2+ signal transduction and Na+ transport ability, salinity tolerance-related transcription factors and antioxidant ability, an increase of auxin, and inhibition of cytokinin may play key roles in CIAT11365 response to salinity stress. The results of this study may contribute to our understanding of the molecular mechanism underlying the responses of S. guianensis to salinity stress and also provide important clues for further study and in-depth characterization of salinity resistance breeding candidate genes in S. guianensis.
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| Format: | Journal Article biblioteca |
| Language: | English |
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Frontiers Media
2022-03
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| Subjects: | stylosanthes guianensis, salt tolerance, transcription factors, screening, plant breeding, forage, tolerancia a la sal, factores de transcripción, horticulture, ecology, food science, |
| Online Access: | https://hdl.handle.net/10568/121137 https://doi.org/10.3389/fsufs.2022.725656 |
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dig-cgspace-10568-1211372023-12-08T19:36:04Z A transcriptomic analysis of Stylo [Stylosanthes guianensis (Aubl.) Sw.] provides novel insights into the basis of salinity tolerance Liu, Yiming Kong, Danyu Yang, Hubiao Douxchamps, Sabine Atieno, Mary Xu, Bin Wang, Wenqiang Liu, Guodao stylosanthes guianensis salt tolerance transcription factors screening plant breeding forage tolerancia a la sal factores de transcripción horticulture ecology food science Tropical areas have a large distribution of saline soils and tidal flats with a high salinity level. Salinity stress is a key factor limiting the widespread use of tropical forage such as Stylosanthes guianensis (Aubl.) Sw. This study was designed to screen the salinity tolerance of 84 S. guianensis accessions; In a greenhouse experiment, plants were subjected to Hoagland solution or Hoagland solution with 200 mM NaCl for up to 15 days. Salinity tolerant accession CIAT11365 and salinity sensitive accession FM05-2 were obtained based on withered leaf rate (WLR). Further verification of salinity tolerance in CIAT11365 and FM05-2 with different salinity gradients showed that salinity stress increased WLR and decreased relative chlorophyll content (SPAD), maximum photochemical efficiency of photosystem II (Fv/Fm), and photosynthetic rate (Pn) in FM05-2, but CIAT11365 exhibited lower WLR and higher SPAD, Fv/Fm, and Pn. Leaf RNA-Seq revealed that Ca2+ signal transduction and Na+ transport ability, salinity tolerance-related transcription factors and antioxidant ability, an increase of auxin, and inhibition of cytokinin may play key roles in CIAT11365 response to salinity stress. The results of this study may contribute to our understanding of the molecular mechanism underlying the responses of S. guianensis to salinity stress and also provide important clues for further study and in-depth characterization of salinity resistance breeding candidate genes in S. guianensis. 2022-03 2022-09-06T14:44:15Z 2022-09-06T14:44:15Z Journal Article Liu, Y.; Kong, D.; Yang, H.; Douxchamps, S.; Atieno, M.; Xu, B; Wang, W.; Liu, G. (2022) A transcriptomic analysis of Stylo [Stylosanthes guianensis (Aubl.) Sw.] provides novel insights into the basis of salinity tolerance. Frontiers in Sustainable Food Systems 6:725656. ISSN: 2571-581X 2571-581X https://hdl.handle.net/10568/121137 https://doi.org/10.3389/fsufs.2022.725656 en CC-BY-4.0 Open Access application/pdf Frontiers Media Frontiers in Sustainable Food Systems |
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stylosanthes guianensis salt tolerance transcription factors screening plant breeding forage tolerancia a la sal factores de transcripción horticulture ecology food science stylosanthes guianensis salt tolerance transcription factors screening plant breeding forage tolerancia a la sal factores de transcripción horticulture ecology food science |
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stylosanthes guianensis salt tolerance transcription factors screening plant breeding forage tolerancia a la sal factores de transcripción horticulture ecology food science stylosanthes guianensis salt tolerance transcription factors screening plant breeding forage tolerancia a la sal factores de transcripción horticulture ecology food science Liu, Yiming Kong, Danyu Yang, Hubiao Douxchamps, Sabine Atieno, Mary Xu, Bin Wang, Wenqiang Liu, Guodao A transcriptomic analysis of Stylo [Stylosanthes guianensis (Aubl.) Sw.] provides novel insights into the basis of salinity tolerance |
| description |
Tropical areas have a large distribution of saline soils and tidal flats with a high salinity level. Salinity stress is a key factor limiting the widespread use of tropical forage such as Stylosanthes guianensis (Aubl.) Sw. This study was designed to screen the salinity tolerance of 84 S. guianensis accessions; In a greenhouse experiment, plants were subjected to Hoagland solution or Hoagland solution with 200 mM NaCl for up to 15 days. Salinity tolerant accession CIAT11365 and salinity sensitive accession FM05-2 were obtained based on withered leaf rate (WLR). Further verification of salinity tolerance in CIAT11365 and FM05-2 with different salinity gradients showed that salinity stress increased WLR and decreased relative chlorophyll content (SPAD), maximum photochemical efficiency of photosystem II (Fv/Fm), and photosynthetic rate (Pn) in FM05-2, but CIAT11365 exhibited lower WLR and higher SPAD, Fv/Fm, and Pn. Leaf RNA-Seq revealed that Ca2+ signal transduction and Na+ transport ability, salinity tolerance-related transcription factors and antioxidant ability, an increase of auxin, and inhibition of cytokinin may play key roles in CIAT11365 response to salinity stress. The results of this study may contribute to our understanding of the molecular mechanism underlying the responses of S. guianensis to salinity stress and also provide important clues for further study and in-depth characterization of salinity resistance breeding candidate genes in S. guianensis. |
| format |
Journal Article |
| topic_facet |
stylosanthes guianensis salt tolerance transcription factors screening plant breeding forage tolerancia a la sal factores de transcripción horticulture ecology food science |
| author |
Liu, Yiming Kong, Danyu Yang, Hubiao Douxchamps, Sabine Atieno, Mary Xu, Bin Wang, Wenqiang Liu, Guodao |
| author_facet |
Liu, Yiming Kong, Danyu Yang, Hubiao Douxchamps, Sabine Atieno, Mary Xu, Bin Wang, Wenqiang Liu, Guodao |
| author_sort |
Liu, Yiming |
| title |
A transcriptomic analysis of Stylo [Stylosanthes guianensis (Aubl.) Sw.] provides novel insights into the basis of salinity tolerance |
| title_short |
A transcriptomic analysis of Stylo [Stylosanthes guianensis (Aubl.) Sw.] provides novel insights into the basis of salinity tolerance |
| title_full |
A transcriptomic analysis of Stylo [Stylosanthes guianensis (Aubl.) Sw.] provides novel insights into the basis of salinity tolerance |
| title_fullStr |
A transcriptomic analysis of Stylo [Stylosanthes guianensis (Aubl.) Sw.] provides novel insights into the basis of salinity tolerance |
| title_full_unstemmed |
A transcriptomic analysis of Stylo [Stylosanthes guianensis (Aubl.) Sw.] provides novel insights into the basis of salinity tolerance |
| title_sort |
transcriptomic analysis of stylo [stylosanthes guianensis (aubl.) sw.] provides novel insights into the basis of salinity tolerance |
| publisher |
Frontiers Media |
| publishDate |
2022-03 |
| url |
https://hdl.handle.net/10568/121137 https://doi.org/10.3389/fsufs.2022.725656 |
| work_keys_str_mv |
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