Prevalence, antibiogram, and multidrug-resistant profile of E. coli O157: H7 in retail raw beef in Addis Ababa, Ethiopia
Escherichia coli O157:H7 is an emerging foodborne pathogen of public health importance. The objectives of this study were to estimate the prevalence and evaluate the antimicrobial susceptibility pattern and multidrug-resistant profile of E. coli O157:H7 isolated from raw beef sold in butcher shops in Addis Ababa, Ethiopia. A total of 384 raw beef samples were collected from randomly selected butcher shops across the 10 sub-cities of Addis Ababa. E. coli O157:H7 was isolated following ISO-16654:2001 standard, and isolates were tested for resistance to 13 antimicrobial agents using the Kirby–Bauer disk diffusion method. Out of the 384 retail raw beef samples examined, 14 (3.64%) (95% CI = 1.77–5.51%) carried E. coli O157:H7 serotype. Of the 14 E. coli O157:H7 isolates, 8 (57.14%) were found to be resistant to three or more antimicrobial categories. The frequency of resistant phenotype was more common for ampicillin (92.8%), nitrofurantoin (92.8%), and tetracycline (50%). Multidrug-resistant E. coli O157:H7 were present in raw beef sold in butcher shops in Addis Ababa. Thus, more stringent monitoring of antimicrobial use in both human and animal populations should be implemented. In addition, further studies should be conducted to understand the E. coli O157:H7 points of contamination and define appropriate risk mitigation strategies.
Main Authors: | , , , , , |
---|---|
Format: | Journal Article biblioteca |
Language: | English |
Published: |
Frontiers Media
2022-02-24
|
Subjects: | antimicrobial resistance, food safety, animal products, meat, |
Online Access: | https://hdl.handle.net/10568/118241 https://doi.org/10.3389/fvets.2022.734896 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cgspace-10568-118241 |
---|---|
record_format |
koha |
spelling |
dig-cgspace-10568-1182412023-12-08T19:36:04Z Prevalence, antibiogram, and multidrug-resistant profile of E. coli O157: H7 in retail raw beef in Addis Ababa, Ethiopia Haile, A.F. Alonso, Silvia Belay, N. Atoma, T.B. Boyaka, P.N. Grace, Delia antimicrobial resistance food safety animal products meat Escherichia coli O157:H7 is an emerging foodborne pathogen of public health importance. The objectives of this study were to estimate the prevalence and evaluate the antimicrobial susceptibility pattern and multidrug-resistant profile of E. coli O157:H7 isolated from raw beef sold in butcher shops in Addis Ababa, Ethiopia. A total of 384 raw beef samples were collected from randomly selected butcher shops across the 10 sub-cities of Addis Ababa. E. coli O157:H7 was isolated following ISO-16654:2001 standard, and isolates were tested for resistance to 13 antimicrobial agents using the Kirby–Bauer disk diffusion method. Out of the 384 retail raw beef samples examined, 14 (3.64%) (95% CI = 1.77–5.51%) carried E. coli O157:H7 serotype. Of the 14 E. coli O157:H7 isolates, 8 (57.14%) were found to be resistant to three or more antimicrobial categories. The frequency of resistant phenotype was more common for ampicillin (92.8%), nitrofurantoin (92.8%), and tetracycline (50%). Multidrug-resistant E. coli O157:H7 were present in raw beef sold in butcher shops in Addis Ababa. Thus, more stringent monitoring of antimicrobial use in both human and animal populations should be implemented. In addition, further studies should be conducted to understand the E. coli O157:H7 points of contamination and define appropriate risk mitigation strategies. 2022-02-24 2022-02-24T10:46:12Z 2022-02-24T10:46:12Z Journal Article Haile, A.F., Alonso, S., Belay, N., Atoma, T.B., Boyaka, P.N. and Grace, D. 2022. Prevalence, antibiogram, and multidrug-resistant profile of E. coli O157: H7 in retail raw beef in Addis Ababa, Ethiopia. Frontiers in Veterinary Science 9: 734896. 2297-1769 https://hdl.handle.net/10568/118241 https://doi.org/10.3389/fvets.2022.734896 en CC-BY-4.0 Open Access 734896 Frontiers Media Frontiers in Veterinary Science |
institution |
CGIAR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cgspace |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CGIAR |
language |
English |
topic |
antimicrobial resistance food safety animal products meat antimicrobial resistance food safety animal products meat |
spellingShingle |
antimicrobial resistance food safety animal products meat antimicrobial resistance food safety animal products meat Haile, A.F. Alonso, Silvia Belay, N. Atoma, T.B. Boyaka, P.N. Grace, Delia Prevalence, antibiogram, and multidrug-resistant profile of E. coli O157: H7 in retail raw beef in Addis Ababa, Ethiopia |
description |
Escherichia coli O157:H7 is an emerging foodborne pathogen of public health importance. The objectives of this study were to estimate the prevalence and evaluate the antimicrobial susceptibility pattern and multidrug-resistant profile of E. coli O157:H7 isolated from raw beef sold in butcher shops in Addis Ababa, Ethiopia. A total of 384 raw beef samples were collected from randomly selected butcher shops across the 10 sub-cities of Addis Ababa. E. coli O157:H7 was isolated following ISO-16654:2001 standard, and isolates were tested for resistance to 13 antimicrobial agents using the Kirby–Bauer disk diffusion method. Out of the 384 retail raw beef samples examined, 14 (3.64%) (95% CI = 1.77–5.51%) carried E. coli O157:H7 serotype. Of the 14 E. coli O157:H7 isolates, 8 (57.14%) were found to be resistant to three or more antimicrobial categories. The frequency of resistant phenotype was more common for ampicillin (92.8%), nitrofurantoin (92.8%), and tetracycline (50%). Multidrug-resistant E. coli O157:H7 were present in raw beef sold in butcher shops in Addis Ababa. Thus, more stringent monitoring of antimicrobial use in both human and animal populations should be implemented. In addition, further studies should be conducted to understand the E. coli O157:H7 points of contamination and define appropriate risk mitigation strategies. |
format |
Journal Article |
topic_facet |
antimicrobial resistance food safety animal products meat |
author |
Haile, A.F. Alonso, Silvia Belay, N. Atoma, T.B. Boyaka, P.N. Grace, Delia |
author_facet |
Haile, A.F. Alonso, Silvia Belay, N. Atoma, T.B. Boyaka, P.N. Grace, Delia |
author_sort |
Haile, A.F. |
title |
Prevalence, antibiogram, and multidrug-resistant profile of E. coli O157: H7 in retail raw beef in Addis Ababa, Ethiopia |
title_short |
Prevalence, antibiogram, and multidrug-resistant profile of E. coli O157: H7 in retail raw beef in Addis Ababa, Ethiopia |
title_full |
Prevalence, antibiogram, and multidrug-resistant profile of E. coli O157: H7 in retail raw beef in Addis Ababa, Ethiopia |
title_fullStr |
Prevalence, antibiogram, and multidrug-resistant profile of E. coli O157: H7 in retail raw beef in Addis Ababa, Ethiopia |
title_full_unstemmed |
Prevalence, antibiogram, and multidrug-resistant profile of E. coli O157: H7 in retail raw beef in Addis Ababa, Ethiopia |
title_sort |
prevalence, antibiogram, and multidrug-resistant profile of e. coli o157: h7 in retail raw beef in addis ababa, ethiopia |
publisher |
Frontiers Media |
publishDate |
2022-02-24 |
url |
https://hdl.handle.net/10568/118241 https://doi.org/10.3389/fvets.2022.734896 |
work_keys_str_mv |
AT haileaf prevalenceantibiogramandmultidrugresistantprofileofecolio157h7inretailrawbeefinaddisababaethiopia AT alonsosilvia prevalenceantibiogramandmultidrugresistantprofileofecolio157h7inretailrawbeefinaddisababaethiopia AT belayn prevalenceantibiogramandmultidrugresistantprofileofecolio157h7inretailrawbeefinaddisababaethiopia AT atomatb prevalenceantibiogramandmultidrugresistantprofileofecolio157h7inretailrawbeefinaddisababaethiopia AT boyakapn prevalenceantibiogramandmultidrugresistantprofileofecolio157h7inretailrawbeefinaddisababaethiopia AT gracedelia prevalenceantibiogramandmultidrugresistantprofileofecolio157h7inretailrawbeefinaddisababaethiopia |
_version_ |
1787231663603843072 |