Discovery of a major QTL for root-knot nematode (Meloidogyne incognita) resistance in cultivated sweetpotato (Ipomoea batatas)
The root-knot nematode [Meloidogyne incognita (Kofoid & White) Chitwood] (RKN) causes significant storage root quality reduction and yields losses in cultivated sweetpotato [Ipomoea batatas (L.) Lam.]. In this study, resistance to RKN was examined in a mapping population consisting of 244 progenies derived from a cross (TB) between ‘Tanzania,’ a predominant African landrace cultivar with resistance to RKN, and ‘Beauregard,’ an RKN susceptible major cultivar in the USA. We performed quantitative trait loci (QTL) analysis using a random-effect QTL mapping model on the TB genetic map. An RKN bioassay incorporating potted cuttings of each genotype was conducted in the greenhouse and replicated five times over a period of 10 weeks. For each replication, each genotype was inoculated with ca. 20,000 RKN eggs, and root-knot galls were counted ~62 days after inoculation. Resistance to RKN in the progeny was highly skewed toward the resistant parent, exhibiting medium to high levels of resistance. We identified one major QTL on linkage group 7, dominant in nature, which explained 58.3% of the phenotypic variation in RKN counts. This work represents a significant step forward in our understanding of the genetic architecture of RKN resistance and sets the stage for future utilization of genomics-assisted breeding in sweetpotato breeding programs.
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Springer
2021-07
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Subjects: | meloidogyne incognita, roots, quantitative trait loci, ipomoea batatas, genetics, sweet potatoes, |
Online Access: | https://hdl.handle.net/10568/114950 https://doi.org/10.1007/s00122-021-03797-z |
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dig-cgspace-10568-1149502023-12-08T19:36:04Z Discovery of a major QTL for root-knot nematode (Meloidogyne incognita) resistance in cultivated sweetpotato (Ipomoea batatas) Oloka, B.M. Silva Pereira, G. da Amankwaah, V.A. Mollinari, M. Pecota, K.V. Yada, B. Olukolu, B.A. Zeng, Z.B. Craig Yencho, G. meloidogyne incognita roots quantitative trait loci ipomoea batatas genetics sweet potatoes The root-knot nematode [Meloidogyne incognita (Kofoid & White) Chitwood] (RKN) causes significant storage root quality reduction and yields losses in cultivated sweetpotato [Ipomoea batatas (L.) Lam.]. In this study, resistance to RKN was examined in a mapping population consisting of 244 progenies derived from a cross (TB) between ‘Tanzania,’ a predominant African landrace cultivar with resistance to RKN, and ‘Beauregard,’ an RKN susceptible major cultivar in the USA. We performed quantitative trait loci (QTL) analysis using a random-effect QTL mapping model on the TB genetic map. An RKN bioassay incorporating potted cuttings of each genotype was conducted in the greenhouse and replicated five times over a period of 10 weeks. For each replication, each genotype was inoculated with ca. 20,000 RKN eggs, and root-knot galls were counted ~62 days after inoculation. Resistance to RKN in the progeny was highly skewed toward the resistant parent, exhibiting medium to high levels of resistance. We identified one major QTL on linkage group 7, dominant in nature, which explained 58.3% of the phenotypic variation in RKN counts. This work represents a significant step forward in our understanding of the genetic architecture of RKN resistance and sets the stage for future utilization of genomics-assisted breeding in sweetpotato breeding programs. 2021-07 2021-09-11T01:59:37Z 2021-09-11T01:59:37Z Journal Article Oloka, B. M., da Silva Pereira, G., Amankwaah, V. A., Mollinari, M., Pecota, K. V., Yada, B., Olukolu, B. A., Zeng, Z. B., & Craig Yencho, G. (2021b). Discovery of a major QTL for root-knot nematode (Meloidogyne incognita) resistance in cultivated sweetpotato (Ipomoea batatas). Theoretical and Applied Genetics. ISSN 1432-2242. 134(7). 1945–1955. 1432-2242 https://hdl.handle.net/10568/114950 https://doi.org/10.1007/s00122-021-03797-z en CC-BY-4.0 Open Access 1945-1955 Springer Theoretical and Applied Genetics volume |
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meloidogyne incognita roots quantitative trait loci ipomoea batatas genetics sweet potatoes meloidogyne incognita roots quantitative trait loci ipomoea batatas genetics sweet potatoes |
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meloidogyne incognita roots quantitative trait loci ipomoea batatas genetics sweet potatoes meloidogyne incognita roots quantitative trait loci ipomoea batatas genetics sweet potatoes Oloka, B.M. Silva Pereira, G. da Amankwaah, V.A. Mollinari, M. Pecota, K.V. Yada, B. Olukolu, B.A. Zeng, Z.B. Craig Yencho, G. Discovery of a major QTL for root-knot nematode (Meloidogyne incognita) resistance in cultivated sweetpotato (Ipomoea batatas) |
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The root-knot nematode [Meloidogyne incognita (Kofoid & White) Chitwood] (RKN) causes significant storage root quality reduction and yields losses in cultivated sweetpotato [Ipomoea batatas (L.) Lam.]. In this study, resistance to RKN was examined in a mapping population consisting of 244 progenies derived from a cross (TB) between ‘Tanzania,’ a predominant African landrace cultivar with resistance to RKN, and ‘Beauregard,’ an RKN susceptible major cultivar in the USA. We performed quantitative trait loci (QTL) analysis using a random-effect QTL mapping model on the TB genetic map. An RKN bioassay incorporating potted cuttings of each genotype was conducted in the greenhouse and replicated five times over a period of 10 weeks. For each replication, each genotype was inoculated with ca. 20,000 RKN eggs, and root-knot galls were counted ~62 days after inoculation. Resistance to RKN in the progeny was highly skewed toward the resistant parent, exhibiting medium to high levels of resistance. We identified one major QTL on linkage group 7, dominant in nature, which explained 58.3% of the phenotypic variation in RKN counts. This work represents a significant step forward in our understanding of the genetic architecture of RKN resistance and sets the stage for future utilization of genomics-assisted breeding in sweetpotato breeding programs. |
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Journal Article |
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meloidogyne incognita roots quantitative trait loci ipomoea batatas genetics sweet potatoes |
author |
Oloka, B.M. Silva Pereira, G. da Amankwaah, V.A. Mollinari, M. Pecota, K.V. Yada, B. Olukolu, B.A. Zeng, Z.B. Craig Yencho, G. |
author_facet |
Oloka, B.M. Silva Pereira, G. da Amankwaah, V.A. Mollinari, M. Pecota, K.V. Yada, B. Olukolu, B.A. Zeng, Z.B. Craig Yencho, G. |
author_sort |
Oloka, B.M. |
title |
Discovery of a major QTL for root-knot nematode (Meloidogyne incognita) resistance in cultivated sweetpotato (Ipomoea batatas) |
title_short |
Discovery of a major QTL for root-knot nematode (Meloidogyne incognita) resistance in cultivated sweetpotato (Ipomoea batatas) |
title_full |
Discovery of a major QTL for root-knot nematode (Meloidogyne incognita) resistance in cultivated sweetpotato (Ipomoea batatas) |
title_fullStr |
Discovery of a major QTL for root-knot nematode (Meloidogyne incognita) resistance in cultivated sweetpotato (Ipomoea batatas) |
title_full_unstemmed |
Discovery of a major QTL for root-knot nematode (Meloidogyne incognita) resistance in cultivated sweetpotato (Ipomoea batatas) |
title_sort |
discovery of a major qtl for root-knot nematode (meloidogyne incognita) resistance in cultivated sweetpotato (ipomoea batatas) |
publisher |
Springer |
publishDate |
2021-07 |
url |
https://hdl.handle.net/10568/114950 https://doi.org/10.1007/s00122-021-03797-z |
work_keys_str_mv |
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