Three-gene PCR and high-resolution melting analysis for differentiating vertebrate species mitochondrial DNA for biodiversity research and complementing forensic surveillance
Reliable molecular identification of vertebrate species from morphologically unidentifiable tissue is critical for the prosecution of illegally-traded wildlife products, conservation-based biodiversity research, and identification of blood-meal hosts of hematophagous invertebrates. However, forensic identification of vertebrate tissue relies on sequencing of the mitochondrial cytochrome oxidase I (COI) ‘barcode’ gene, which remains costly for purposes of screening large numbers of unknown samples during routine surveillance. Here, we adapted a rapid, low-cost approach to differentiate 10 domestic and 24 wildlife species that are common in the East African illegal wildlife products trade based on their unique high-resolution melting profiles from COI, cytochrome b, and 16S ribosomal RNA gene PCR products. Using the approach, we identified (i) giraffe among covertly sampled meat from Kenyan butcheries, and (ii) forest elephant mitochondrial sequences among savannah elephant reference samples. This approach is being adopted for high-throughput pre-screening of potential bushmeat samples in East African forensic science pipelines.
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| Format: | Journal Article biblioteca |
| Language: | English |
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Springer
2020-03-16
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| Subjects: | biodiversity, research, pcr, wildlife, forensic sciences, |
| Online Access: | https://hdl.handle.net/10568/108124 https://doi.org/10.1038/s41598-020-61600-3 |
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dig-cgspace-10568-1081242023-12-08T19:36:04Z Three-gene PCR and high-resolution melting analysis for differentiating vertebrate species mitochondrial DNA for biodiversity research and complementing forensic surveillance Ouso, D.O. Otiende, M.Y. Jeneby, M.M. Oundo, J.W. Bargul, J.L. Miller, S.E. Wambua, Lillian Villinger, J. biodiversity research pcr wildlife forensic sciences Reliable molecular identification of vertebrate species from morphologically unidentifiable tissue is critical for the prosecution of illegally-traded wildlife products, conservation-based biodiversity research, and identification of blood-meal hosts of hematophagous invertebrates. However, forensic identification of vertebrate tissue relies on sequencing of the mitochondrial cytochrome oxidase I (COI) ‘barcode’ gene, which remains costly for purposes of screening large numbers of unknown samples during routine surveillance. Here, we adapted a rapid, low-cost approach to differentiate 10 domestic and 24 wildlife species that are common in the East African illegal wildlife products trade based on their unique high-resolution melting profiles from COI, cytochrome b, and 16S ribosomal RNA gene PCR products. Using the approach, we identified (i) giraffe among covertly sampled meat from Kenyan butcheries, and (ii) forest elephant mitochondrial sequences among savannah elephant reference samples. This approach is being adopted for high-throughput pre-screening of potential bushmeat samples in East African forensic science pipelines. 2020-03-16 2020-05-01T12:49:14Z 2020-05-01T12:49:14Z Journal Article Ouso, D.O., Otiende, M.Y., Jeneby, M.M., Oundo, J.W., Bargul, J.L., Miller, S.E., Wambua, L. and Villinger, J. 2020. Three-gene PCR and high-resolution melting analysis for differentiating vertebrate species mitochondrial DNA for biodiversity research and complementing forensic surveillance. Scientific Reports 10: 4741. 2045-2322 https://hdl.handle.net/10568/108124 https://doi.org/10.1038/s41598-020-61600-3 en CC-BY-4.0 Open Access Springer Scientific Reports |
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biodiversity research pcr wildlife forensic sciences biodiversity research pcr wildlife forensic sciences |
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biodiversity research pcr wildlife forensic sciences biodiversity research pcr wildlife forensic sciences Ouso, D.O. Otiende, M.Y. Jeneby, M.M. Oundo, J.W. Bargul, J.L. Miller, S.E. Wambua, Lillian Villinger, J. Three-gene PCR and high-resolution melting analysis for differentiating vertebrate species mitochondrial DNA for biodiversity research and complementing forensic surveillance |
| description |
Reliable molecular identification of vertebrate species from morphologically unidentifiable tissue is critical for the prosecution of illegally-traded wildlife products, conservation-based biodiversity research, and identification of blood-meal hosts of hematophagous invertebrates. However, forensic identification of vertebrate tissue relies on sequencing of the mitochondrial cytochrome oxidase I (COI) ‘barcode’ gene, which remains costly for purposes of screening large numbers of unknown samples during routine surveillance. Here, we adapted a rapid, low-cost approach to differentiate 10 domestic and 24 wildlife species that are common in the East African illegal wildlife products trade based on their unique high-resolution melting profiles from COI, cytochrome b, and 16S ribosomal RNA gene PCR products. Using the approach, we identified (i) giraffe among covertly sampled meat from Kenyan butcheries, and (ii) forest elephant mitochondrial sequences among savannah elephant reference samples. This approach is being adopted for high-throughput pre-screening of potential bushmeat samples in East African forensic science pipelines. |
| format |
Journal Article |
| topic_facet |
biodiversity research pcr wildlife forensic sciences |
| author |
Ouso, D.O. Otiende, M.Y. Jeneby, M.M. Oundo, J.W. Bargul, J.L. Miller, S.E. Wambua, Lillian Villinger, J. |
| author_facet |
Ouso, D.O. Otiende, M.Y. Jeneby, M.M. Oundo, J.W. Bargul, J.L. Miller, S.E. Wambua, Lillian Villinger, J. |
| author_sort |
Ouso, D.O. |
| title |
Three-gene PCR and high-resolution melting analysis for differentiating vertebrate species mitochondrial DNA for biodiversity research and complementing forensic surveillance |
| title_short |
Three-gene PCR and high-resolution melting analysis for differentiating vertebrate species mitochondrial DNA for biodiversity research and complementing forensic surveillance |
| title_full |
Three-gene PCR and high-resolution melting analysis for differentiating vertebrate species mitochondrial DNA for biodiversity research and complementing forensic surveillance |
| title_fullStr |
Three-gene PCR and high-resolution melting analysis for differentiating vertebrate species mitochondrial DNA for biodiversity research and complementing forensic surveillance |
| title_full_unstemmed |
Three-gene PCR and high-resolution melting analysis for differentiating vertebrate species mitochondrial DNA for biodiversity research and complementing forensic surveillance |
| title_sort |
three-gene pcr and high-resolution melting analysis for differentiating vertebrate species mitochondrial dna for biodiversity research and complementing forensic surveillance |
| publisher |
Springer |
| publishDate |
2020-03-16 |
| url |
https://hdl.handle.net/10568/108124 https://doi.org/10.1038/s41598-020-61600-3 |
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