Transcriptomic analysis of resistant and susceptible banana corms in response to infection by Fusarium oxysporum f. sp. cubense tropical race 4
Fusarium wilt disease, caused by Fusarium oxysporum f. sp. cubense, especially by tropical race 4 (Foc TR4), is threatening the global banana industry. Musa acuminata Pahang, a wild diploid banana that displays strong resistance to Foc TR4, holds great potential to understand the underlying resistance mechanisms. Microscopic examination reports that, in a wounding inoculation system, the Foc TR4 infection processes in roots of Pahang (resistant) and a triploid cultivar Brazilian (susceptible) were similar by 7 days post inoculation (dpi), but significant differences were observed in corms of both genotypes at 14 dpi. We compare transcriptomic responses in the corms of Pahang and Brazilian, and show that Pahang exhibited constitutive defense responses before Foc TR4 infection and inducible defense responses prior to Brazilian at the initial Foc TR4 infection stage. Most key enzymatic genes in the phenylalanine metabolism pathway were up-regulated in Brazilian, suggesting that lignin and phytotoxin may be triggered during later stages of Foc TR4 infection. This study unravels a few potential resistance candidate genes whose expression patterns were assessed by RT-qPCR assay and improves our understanding the defense mechanisms of Pahang response to Foc TR4.
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Format: | Journal Article biblioteca |
Language: | English |
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Springer
2019-01-01
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Subjects: | fusarium oxysporum, bananas, disease resistance, gene expression, musa, panama disease, transcription, |
Online Access: | https://hdl.handle.net/10568/101508 https://doi.org/10.1038/s41598-019-44637-x https://doi.org/10.7910/DVN/W2M6WG |
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dig-cgspace-10568-1015082023-12-27T19:38:22Z Transcriptomic analysis of resistant and susceptible banana corms in response to infection by Fusarium oxysporum f. sp. cubense tropical race 4 Zhang, L. Cenci, A. Rouard, M. Zhang, D. Wang, Y. Tang, W. Zheng, S.J. fusarium oxysporum bananas disease resistance gene expression musa panama disease transcription Fusarium wilt disease, caused by Fusarium oxysporum f. sp. cubense, especially by tropical race 4 (Foc TR4), is threatening the global banana industry. Musa acuminata Pahang, a wild diploid banana that displays strong resistance to Foc TR4, holds great potential to understand the underlying resistance mechanisms. Microscopic examination reports that, in a wounding inoculation system, the Foc TR4 infection processes in roots of Pahang (resistant) and a triploid cultivar Brazilian (susceptible) were similar by 7 days post inoculation (dpi), but significant differences were observed in corms of both genotypes at 14 dpi. We compare transcriptomic responses in the corms of Pahang and Brazilian, and show that Pahang exhibited constitutive defense responses before Foc TR4 infection and inducible defense responses prior to Brazilian at the initial Foc TR4 infection stage. Most key enzymatic genes in the phenylalanine metabolism pathway were up-regulated in Brazilian, suggesting that lignin and phytotoxin may be triggered during later stages of Foc TR4 infection. This study unravels a few potential resistance candidate genes whose expression patterns were assessed by RT-qPCR assay and improves our understanding the defense mechanisms of Pahang response to Foc TR4. 2019-01-01 2019-06-10T12:22:09Z 2019-06-10T12:22:09Z Journal Article Zhang, L.; Cenci, A.; Rouard, M.; Zhang, D.; Wang, Y.; Tang, W.; Zheng, S.J. (2019) Transcriptomic analysis of resistant and susceptible banana corms in response to infection by Fusarium oxysporum f. sp. cubense tropical race 4. Scientific Reports, 9, Article number: 8199. ISSN: 2045-2322. 2045-2322 https://hdl.handle.net/10568/101508 https://doi.org/10.1038/s41598-019-44637-x https://doi.org/10.7910/DVN/W2M6WG en CC-BY-4.0 Open Access application/pdf Springer Scientific Reports |
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fusarium oxysporum bananas disease resistance gene expression musa panama disease transcription fusarium oxysporum bananas disease resistance gene expression musa panama disease transcription |
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fusarium oxysporum bananas disease resistance gene expression musa panama disease transcription fusarium oxysporum bananas disease resistance gene expression musa panama disease transcription Zhang, L. Cenci, A. Rouard, M. Zhang, D. Wang, Y. Tang, W. Zheng, S.J. Transcriptomic analysis of resistant and susceptible banana corms in response to infection by Fusarium oxysporum f. sp. cubense tropical race 4 |
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Fusarium wilt disease, caused by Fusarium oxysporum f. sp. cubense, especially by tropical race 4 (Foc TR4), is threatening the global banana industry. Musa acuminata Pahang, a wild diploid banana that displays strong resistance to Foc TR4, holds great potential to understand the underlying resistance mechanisms. Microscopic examination reports that, in a wounding inoculation system, the Foc TR4 infection processes in roots of Pahang (resistant) and a triploid cultivar Brazilian (susceptible) were similar by 7 days post inoculation (dpi), but significant differences were observed in corms of both genotypes at 14 dpi. We compare transcriptomic responses in the corms of Pahang and Brazilian, and show that Pahang exhibited constitutive defense responses before Foc TR4 infection and inducible defense responses prior to Brazilian at the initial Foc TR4 infection stage. Most key enzymatic genes in the phenylalanine metabolism pathway were up-regulated in Brazilian, suggesting that lignin and phytotoxin may be triggered during later stages of Foc TR4 infection. This study unravels a few potential resistance candidate genes whose expression patterns were assessed by RT-qPCR assay and improves our understanding the defense mechanisms of Pahang response to Foc TR4. |
format |
Journal Article |
topic_facet |
fusarium oxysporum bananas disease resistance gene expression musa panama disease transcription |
author |
Zhang, L. Cenci, A. Rouard, M. Zhang, D. Wang, Y. Tang, W. Zheng, S.J. |
author_facet |
Zhang, L. Cenci, A. Rouard, M. Zhang, D. Wang, Y. Tang, W. Zheng, S.J. |
author_sort |
Zhang, L. |
title |
Transcriptomic analysis of resistant and susceptible banana corms in response to infection by Fusarium oxysporum f. sp. cubense tropical race 4 |
title_short |
Transcriptomic analysis of resistant and susceptible banana corms in response to infection by Fusarium oxysporum f. sp. cubense tropical race 4 |
title_full |
Transcriptomic analysis of resistant and susceptible banana corms in response to infection by Fusarium oxysporum f. sp. cubense tropical race 4 |
title_fullStr |
Transcriptomic analysis of resistant and susceptible banana corms in response to infection by Fusarium oxysporum f. sp. cubense tropical race 4 |
title_full_unstemmed |
Transcriptomic analysis of resistant and susceptible banana corms in response to infection by Fusarium oxysporum f. sp. cubense tropical race 4 |
title_sort |
transcriptomic analysis of resistant and susceptible banana corms in response to infection by fusarium oxysporum f. sp. cubense tropical race 4 |
publisher |
Springer |
publishDate |
2019-01-01 |
url |
https://hdl.handle.net/10568/101508 https://doi.org/10.1038/s41598-019-44637-x https://doi.org/10.7910/DVN/W2M6WG |
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