Shoot regeneration from peduncles and shoot-like regeneration from leaves of babaco, Carica pentagona
C. pentagona shoots were regenerated from peduncle sections cultured on medium containing benzyladenine (BA, 0, 0.5 or 1.0 mg/litre) plus IAA (0.5 or 1.0 mg/litre) under a 16-h or 18-h photoperiod and a light intensity of 16 micro mol-1 m-2. Whole leaves from in vitro-stock material and leaf sections containing either a petiole or main vein from greenhouse plants regenerated roots and/or shoot-like structures on medium containing BA (1.0 or 1.5 mg/litre) plus IAA (1.0 or 3.0 mg/litre). Histological analysis of sections of peduncles and leaves demonstrated that shoots appeared to regenerate directly from cells associated with the vascular systems.
Main Authors: | , , |
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Format: | Artículo biblioteca |
Language: | English |
Published: |
IICA, San José (Costa Rica)
1993-04
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Subjects: | CARICA PENTAGONA, FRUTALES, CULTIVO DE TEJIDOS, CULTIVO IN VITRO, ORGANOGENESIS, BROTES, SUSTANCIAS DE CRECIMIENTO VEGETAL, 6-BENZILADENINA, AIA, |
Online Access: | https://repositorio.catie.ac.cr/handle/11554/9635 |
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Summary: | C. pentagona shoots were regenerated from peduncle sections cultured on medium containing benzyladenine (BA, 0, 0.5 or 1.0 mg/litre) plus IAA (0.5 or 1.0 mg/litre) under a 16-h or 18-h photoperiod and a light intensity of 16 micro mol-1 m-2. Whole leaves from in vitro-stock material and leaf sections containing either a petiole or main vein from greenhouse plants regenerated roots and/or shoot-like structures on medium containing BA (1.0 or 1.5 mg/litre) plus IAA (1.0 or 3.0 mg/litre). Histological analysis of sections of peduncles and leaves demonstrated that shoots appeared to regenerate directly from cells associated with the vascular systems. |
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