Effects of essential oils of Satureja bachtiarica and Nigella sativa on the efficacy of lactococcosis vaccine in rainbow trout (Oncorhynchus mykiss)
Lactococcosis has been defined as acute septicaemia, which causes economic losses in farmed fish, especially in rainbow trout. This study was done to evaluate the effects of the essential oils of Satureja bachtiarica and Nigella sativa on the efficacy of lactococcosis vaccine in rainbow trout. A total number of 270 fishes with a mean weight of 120 g were obtained; they were randomly divided into nine groups, each with three replicates, after two weeks of adaptation. The groups were: no injection group, vaccine only group, DMSO injection group, vaccine with 50, 100, and 200 micrograms Intraperitoneal injection (IP) injection. Two, four, and six weeks after vaccination, serological and haematological parameters were evaluated. In the sixth week, 1.7×10^7 cfu as LD50 96 hrs of Lactococcus garvieae were IP injected and the relative survival percentage was calculated. The results indicated that N. sativa essence is effective on the leukocyte population as the highest number of leukocytes were found in fish receiving high concentration of N. sativa. The relative survival rate of the studied fish decreased with decreasing concentrations of the N. sativa essential oil concentration, with a significant difference with control groups (p<0.05). However, using S. bachtiarica was not significantly effective on the relative survival rate of fish. The results of this study indicated that N. sativa essential oil can be used as adjuvant for L. garvieae vaccine, since it resulted in increasing leukocytes and the relative survival rate although S. bachtiarica was not effective on immune parameters of the studied fish.
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Language: | English |
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2018
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Subjects: | Aquaculture, Biology, Fisheries, Nigella sativa, Satureja bachtiarica, Streptococcosis, Lactococcosis, Adjuvant, Iran, |
Online Access: | http://hdl.handle.net/1834/37902 |
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dig-aquadocs-1834-379022021-07-13T02:22:42Z Effects of essential oils of Satureja bachtiarica and Nigella sativa on the efficacy of lactococcosis vaccine in rainbow trout (Oncorhynchus mykiss) Raissy, M. Hashemi, S. Roushan, M. Jafarian, M. Momtaz, H. Soltani, M. Pirali Kheirabad, E. Aquaculture Biology Fisheries Nigella sativa Satureja bachtiarica Streptococcosis Lactococcosis Adjuvant Iran Lactococcosis has been defined as acute septicaemia, which causes economic losses in farmed fish, especially in rainbow trout. This study was done to evaluate the effects of the essential oils of Satureja bachtiarica and Nigella sativa on the efficacy of lactococcosis vaccine in rainbow trout. A total number of 270 fishes with a mean weight of 120 g were obtained; they were randomly divided into nine groups, each with three replicates, after two weeks of adaptation. The groups were: no injection group, vaccine only group, DMSO injection group, vaccine with 50, 100, and 200 micrograms Intraperitoneal injection (IP) injection. Two, four, and six weeks after vaccination, serological and haematological parameters were evaluated. In the sixth week, 1.7×10^7 cfu as LD50 96 hrs of Lactococcus garvieae were IP injected and the relative survival percentage was calculated. The results indicated that N. sativa essence is effective on the leukocyte population as the highest number of leukocytes were found in fish receiving high concentration of N. sativa. The relative survival rate of the studied fish decreased with decreasing concentrations of the N. sativa essential oil concentration, with a significant difference with control groups (p<0.05). However, using S. bachtiarica was not significantly effective on the relative survival rate of fish. The results of this study indicated that N. sativa essential oil can be used as adjuvant for L. garvieae vaccine, since it resulted in increasing leukocytes and the relative survival rate although S. bachtiarica was not effective on immune parameters of the studied fish. 2021-06-24T18:08:38Z 2021-06-24T18:08:38Z 2018 article TRUE 1562-2916 http://hdl.handle.net/1834/37902 en http://www.ifro.ir application/pdf application/pdf 95-106 http://aquaticcommons.org/id/eprint/23176 18721 2018-06-19 15:40:31 23176 Iranian Fisheries Science Research Institute |
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Aquaculture Biology Fisheries Nigella sativa Satureja bachtiarica Streptococcosis Lactococcosis Adjuvant Iran Aquaculture Biology Fisheries Nigella sativa Satureja bachtiarica Streptococcosis Lactococcosis Adjuvant Iran |
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Aquaculture Biology Fisheries Nigella sativa Satureja bachtiarica Streptococcosis Lactococcosis Adjuvant Iran Aquaculture Biology Fisheries Nigella sativa Satureja bachtiarica Streptococcosis Lactococcosis Adjuvant Iran Raissy, M. Hashemi, S. Roushan, M. Jafarian, M. Momtaz, H. Soltani, M. Pirali Kheirabad, E. Effects of essential oils of Satureja bachtiarica and Nigella sativa on the efficacy of lactococcosis vaccine in rainbow trout (Oncorhynchus mykiss) |
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Lactococcosis has been defined as acute septicaemia, which causes economic losses in farmed fish, especially in rainbow trout. This study was done to evaluate the effects of the essential oils of Satureja bachtiarica and Nigella sativa on the efficacy of lactococcosis vaccine in rainbow trout. A total number of 270 fishes with a mean weight of 120 g were obtained; they were randomly divided into nine groups, each with three replicates, after two weeks of adaptation. The groups were: no injection group, vaccine only group, DMSO injection group, vaccine with 50, 100, and 200 micrograms Intraperitoneal injection (IP) injection. Two, four, and six weeks after vaccination, serological and haematological parameters were evaluated. In the sixth week, 1.7×10^7 cfu as LD50 96 hrs of Lactococcus garvieae were IP injected and the relative survival percentage was calculated. The results indicated that N. sativa essence is effective on the leukocyte population as the highest number of leukocytes were found in fish receiving high concentration of N. sativa. The relative survival rate of the studied fish decreased with decreasing concentrations of the N. sativa essential oil concentration, with a significant difference with control groups (p<0.05). However, using S. bachtiarica was not significantly effective on the relative survival rate of fish. The results of this study indicated that N. sativa essential oil can be used as adjuvant for L. garvieae vaccine, since it resulted in increasing leukocytes and the relative survival rate although S. bachtiarica was not effective on immune parameters of the studied fish. |
format |
article |
topic_facet |
Aquaculture Biology Fisheries Nigella sativa Satureja bachtiarica Streptococcosis Lactococcosis Adjuvant Iran |
author |
Raissy, M. Hashemi, S. Roushan, M. Jafarian, M. Momtaz, H. Soltani, M. Pirali Kheirabad, E. |
author_facet |
Raissy, M. Hashemi, S. Roushan, M. Jafarian, M. Momtaz, H. Soltani, M. Pirali Kheirabad, E. |
author_sort |
Raissy, M. |
title |
Effects of essential oils of Satureja bachtiarica and Nigella sativa on the efficacy of lactococcosis vaccine in rainbow trout (Oncorhynchus mykiss) |
title_short |
Effects of essential oils of Satureja bachtiarica and Nigella sativa on the efficacy of lactococcosis vaccine in rainbow trout (Oncorhynchus mykiss) |
title_full |
Effects of essential oils of Satureja bachtiarica and Nigella sativa on the efficacy of lactococcosis vaccine in rainbow trout (Oncorhynchus mykiss) |
title_fullStr |
Effects of essential oils of Satureja bachtiarica and Nigella sativa on the efficacy of lactococcosis vaccine in rainbow trout (Oncorhynchus mykiss) |
title_full_unstemmed |
Effects of essential oils of Satureja bachtiarica and Nigella sativa on the efficacy of lactococcosis vaccine in rainbow trout (Oncorhynchus mykiss) |
title_sort |
effects of essential oils of satureja bachtiarica and nigella sativa on the efficacy of lactococcosis vaccine in rainbow trout (oncorhynchus mykiss) |
publishDate |
2018 |
url |
http://hdl.handle.net/1834/37902 |
work_keys_str_mv |
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